Hydrolysis of adenosine triphosphate accompanying inter-action between Escherichia coli B restriction endonuclease and unmodified deoxyribonucleic acid.
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Adenosine Triphosphate↗
Biomedical subjects
J Hedgpeth
Publications and source records attributed to J Hedgpeth.
DNA substrate site for the EcoRII restriction endonuclease and modification methylase.
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Avian Sarcoma Viruses↗
DNA nucleotide sequence restricted by the RI endonuclease.
The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed from a pancreatic DNase digest. The following sequence of nucleotides adjacent to the RI break made in lambda DNA was deduced from these data and from the 3'-dinucleotide sequence and nearest-neighbor analysis obtained from repair synthesis with the DNA polymerase of Rous sarcoma virus [Formula: see text] The RI endonuclease cleavage of the phosphodiester bonds (indicated by arrows) generates 5'-phosphoryls and short cohesive termini of four nucleotides, (p)A(p)A(p)T(p)T. The most striking feature of the sequence is its symmetry.
Adenine Nucleotides↗
Induction of poxvirus ribonucleic acid polymerases.
Two distinct ribonucleic acid polymerase activities were induced in HeLa cells by poxvirus infection. These activities differ both in their properties and the time of their appearance after infection. One catalyzes the dAT (copolymer of deoxyadenylate and deoxythymidylate)-primed conversion of adenosine triphosphate and uridine triphosphate into an acid-insoluble product. This enzyme is detectable only if deoxyribonucleic acid synthesis has been blocked. In contrast, the accumulation of progeny genomes is a necessary condition for induction of the second enzyme. The latter activity, which is unmasked by detergent treatment, is found exclusively in maturing virus particles. The possibility that both enzymes are involved in transcribing the viral genome is discussed.
Cycloheximide↗