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Biomedical subjects

J Henkel

Publications and source records attributed to J Henkel.

At least 19 recordsLinked to original sources
PubMed2000

Long term outcome after cervical foraminotomy.

We recently demonstrated the effectiveness of dorsal foraminotomy in lateral herniated cervical disc after 1 year follow-up in a prospective study.(1) The goal of this paper is to confirm these results concerning long term outcome. We carried out a prospective, consecutive study on 54 patients, operated on for lateral herniated cervical disc. We analysed demographic data, the case history, the neurological examination on admission and imaging data. Ninety per cent were followed up for 3.5 years postoperatively. According to their ratings on a pain scale the group were divided into favourable and unfavourable outcomes. These groups were analysed in relation to the patient's initial condition. At follow up, 90% of patients showed complete recovery or improvement. A long standing preoperative neurological deficit seems to be an important prognostic factor for unfavourable long term outcome after cervical foraminotomy.

Adult↗
PubMed1997

A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of African swine fever virus antigens and antibodies.

An improved solid-phase enzyme linked immunosorbent assay (ELISA) using monoclonal antibodies was developed to detect an African swine fever virus protein (VP73) in pig samples. The use of monoclonal antibodies against VP73 allowed a sensitive and specific sandwich ELISA. This assay detected a limiting antigen concentration of 0.05 microgram/ml of VP73, lower than the detection limit of 0.6 microgram/ml obtained by using polyclonal antibodies by the same ELISA. The whole virus particle was detected with this method to a limit of 2.3 x 10(2) PFU/ml. At the same time, an indirect ELISA was developed to detect ASFV antibodies. The results also indicate that this method may be a useful technique for epidemiological surveys.

African Swine Fever↗
PubMed1994

Safeguarding human tissue transplants.

Transplants of human tissues, such as cornea and bone, can be modern miracles. But they can also transmit disease such as HIV and hepatitis. FDA has tightened controls of transplanted human tissue to minimize risks, and is considering additional action.

Humans↗
PubMed1994

[Is prevention of thromboembolism in ambulatory and conservative therapy of rupture of the fibular ligament of the upper ankle joint necessary?].

In a prospective clinical study, 111 outpatients with tears of the lateral ankle ligaments were investigated for the occurrence of deep venous thrombosis (DVT). All patients had been treated by a plaster cast for 1 week and had not received any form of DVT prophylaxis. After removal of the plaster cast (mean 7.1 days), colour flow imaging of the veins of the injured leg was performed. A phlebography was undertaken whenever there was any suspicion of DVT. Risk factors for thrombosis [3, 10] were documented in all patients. Besides injury and immobilisation, a median of 1.2 (0-4) risk factors for DVT was involved. Only 31 patients had no additional risk factors at all. Median age of the patients was 26.1 years (16-53 median range). Only 1 patient over 40 years of age was diagnosed as having DVT, and none under 40. Therefore, medicamentous prophylaxis of DVT is not considered necessary in outpatients younger than 40 years with ankle ligament tears treated by bi-valved casts in cases when there are no additional risk factors (Table 4) for DVT.

Adolescent↗
PubMed1992

Atypical herpes simplex encephalitis: clinical, virologic, and neuropathologic evaluation.

An atypical form of herpes simplex encephalitis produced by HSV-1 documented in the present article demonstrates that (1) prominent EEG abnormality may correlate with subtle increase in signal intensity on MRI; (2) the disease may start with prominent involvement of the cingulate gyri; and (3) viral infection of the brainstem may cause early onset of severe neurologic dysfunction and coma.

Brain Stem↗
PubMed1990

Referral tracking system shows accurate bottom-line assessment.

Rose Medical Center in Denver, Colo., needed a way to track referrals from its many offsite care centers to determine their impact on revenue. Programmers at Rose developed a microbased system, eventually bought by SMS, that measures profitability against investment to become a true decision support tool for hospital executives.

Colorado↗
PubMed1980

The oxygen-dependent deactivation and reactivation of spinach ribulose-1,5-bisphosphate carboxylase.

Ribulose-1,5-bisphosphate carboxylase-oxygenase (3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39) is deactivated by the removal of oxygen, and reversibly reactivated by its readdition to the enzyme solution. A short pulse of oxygen to the anaerobic enzyme solution is sufficient to trigger the reactivation process; the Ka value for this reaction was estimated as 0.12 mM oxygen. The enzyme could not be reactivated under anaerobic conditions by an organic oxidant (benzoylperoxide) or by sulfhydryl group reducing reagents (dithiothreitol or beta-mercaptoethanol), suggesting that the process of reactivation was oxygen specific. Furthermore, the inhibition of the reactivation by superoxide anion scavengers such as Tiron (1,2-dihydroxybenzene-3,5-disulfonic acid), copper penicillamine, hydroxylamine, nitroblue tetrazolium, and ascorbate, indicated that the monovalent reduced oxygen was involved as the reacting species in this process. The deactivation of the enzyme associated with the removal of oxygen was also sensitive to the presence of scavengers of O2(-), suggesting that superoxide anion was also involved in the deactivation process. Both the carboxylase and the oxygenase activities were similarly affected under all the experimental conditions studied. On the basis of these results it is argued that the enzyme molecules are able to reduce oxygen and that superoxide anion causes the deactivation or reactivation of the enzyme.

1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium ↗
PubMedDate not supplied

The activation of ribulose-1,5-bisphosphate carboxylase-oxygenase from spinach by oxygen.

The effect of oxygen on ribulose-1,5-bisphosphate carboxylase-oxygenase from spinach was investigated. Both activities were deactivated by removal of oxygen and reversibly reactivated by oxygenation of the enzyme solution. The change in enzyme activities was accompanied by conformational changes as studied by the use of intrinsic and extrinsic fluorescent probes. The analysis of cysteine sulfhydryl groups accessible to 5,5'-dithiobis-(2-nitrobenzoic acid) revealed that the number of these groups changed with the oxygen concentration. The kinetic of the exposure of eight cysteine residues was similar to the loss of enzyme activities. The modification of these groups with 5,5'-dithiobis-(2-nitrobenzoic acid) caused almost complete loss of both the activities. The enzyme isolated from a photolithotrophic organism, Chromatium vinosum, was not affected by oxygen removal. During air--argon transitions, neither the enzyme conformation nor the number of accessible sulfhydryl groups changed.

Carboxy-Lyases↗