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J Hibberd

Publications and source records attributed to J Hibberd.

9 recordsLinked to original sources
PubMed2001

Novel approach in plastid transformation.

Engineering the nuclear genome of plants is perceived to be associated with problems regarding biosafety and the stability of expression of the transgene. Alternative transformation strategies using the genomic outfit of the plastid promise to be more successful in this respect. Over the past few years progress has been made in screening procedures, and plastid transformation technology has allowed function to be assigned to open reading frames, massive expression of insecticidal agents and proteins involved in herbicide resistance, and the accumulation of biopolymers. Recently, the design of a novel femtoinjection technique that allows injection into chloroplasts has provided the opportunity to further manipulate and understand chloroplastic gene expression.

Chloroplasts↗
PubMed1998

"We picked the wrong one to sterilise": the role of nursing in the eugenics movement in Alberta, 1920-1940.

The opening decades of the twentieth century were years in which the Canadian government actively recruited immigrants from the United Kingdom, Europe and the United States to assist in the settlement of western Canada. The immigration waves that followed contained large numbers of non-English speaking people of eastern European heritage. As these numbers increased, the white Anglo-Saxon minority feared a "multiplication of the inferior". Alberta's solution was the passage of the Sterilization Act in 1928 and the creation of the Eugenics Board. The Act remained law until 1972 and its repercussions are facing Alberta courts today. The Act was supported by many prominent first-wave feminists such as Nellie McClung and Emily Murphy. Nurses too supported the Act and the philosophical ideology on which the need for sterilization was based- "an available means for racial improvement." The role nurses played in promoting eugenics in Alberta through referrals to the Eugenics Board and the promotion of birth control material will be explored in the presentation. Contributions made by public health nurses will be included since much of their work was devoted to the socialization of new immigrants to Canada. Class, gender and ethnicity are central themes to this discussion because the majority of individuals who were sterilized were unemployed, female immigrants from eastern Europe. These two decades represent a time in nursing history when the nurse's duty to care was complicated by the attitudes and values of the society in which she existed. Primary sources used include the personal collection of one of Alberta's early birth control nurses, Canadian Journal of Public Health, Canadian Nurse, and Alberta newspapers.

Canada↗
PubMed1983

Leukocyte subsets in first-set rat cardiac allograft rejection. A serial immunohistologic study using monoclonal antibodies.

For an immunohistochemical analysis of the cells infiltrating rat cardiac allografts undergoing unmodified first-set rejection, functioning Wistar Furth (RT1u) cardiac allografts transplanted to Lewis (RT1(1)) recipients were arbitrarily removed at days 3-6 posttransplantation, and were evaluated histologically using a series of monoclonal antibodies (MAbs) of known cellular distribution, the binding of which was visualized by an indirect immunoperoxidase technique. Sequestered cells present in cryostat sections were segregated numerically on the basis of staining for MAbs detecting T cells, some NK cells, and neutrophils (W3/13); cytotoxic suppressor T cells and most NK cells (MRC OX8); helper T cells and all macrophages (W3/25); B cells and a macrophage subpopulation (MRC OX6, Ia common part determinant); immature T and B cells (MRC OX7, Thy 1.1); and all leukocytes (MRC OX1, LCA). The proportion of cells stained for each MAb was expressed as a percentage of all estimated leukocytes as determined directly on the hematoxylin counterstained sections. Macrophages expressing the W3/25 and MRC OX6 antigens were enumerated on the basis of intracytoplasmic accumulation of previously administered colloidal carbon. There was a significant fall in the relative proportion of W3/25+ carbon- cells within allografts as a function of time. The proportion of mononuclear cells staining for W3/13 and for MRC OX8 showed no statistically significant variation during the period of study. There was a correlation at all periods between the proportional sum of W3/25+ carbon- cells and MRC OX8+ cells and the proportion of W3/13+ mononuclear cells present. The W3/25+ carbon-:MRC OX8+ ratio showed a significant linear decrease with duration of allograft placement. The proportion of carbon+ cells expressing the MRC OX6 and the W3/25 antigens showed a highly significant rise as a function of time. Nonspecific esterase-stained cells showed a similar sequential pattern. These data provide well defined immunohistologic parameters of leukocyte subsets present in unmodified first-set cardiac allograft rejection in the rat.

Animals↗
PubMed1983

A controlled serial ultrastructural tracer study of first-set cardiac allograft rejection in the rat. Evidence that the microvascular endothelium is the primary target of graft destruction.

In order to define the serial morphologic correlates of unmodified first-set cardiac allograft rejection in an inbred rat strain combination, a series of Wistar-Furth cardiac allografts transplanted to normal nonsensitized Lewis recipients were studied as a function of time with the use of well-documented ultrastructural tracer techniques. Colloidial carbon was employed as a vascular label for detection of microvascular endothelial structural alterations, and horseradish peroxidase was used as a tracer probe for localization of cell-membrane permeability dysfunction of allograft endothelium and cardiocytes as well as of elements of the cellular infiltrate. Wistar-Furth to Wistar-Furth syngeneic heart grafts and Wistar-Furth recipients' own hearts provided appropriate control data. This study was demonstrated that severe diffuse loss of functional and structural integrity of the microvascular endothelium precedes the development of extensive damage to cardiac muscle cells and thus provides strong evidence that the allograft microcirculation is the primary target of immunologic injury. In addition, the sequential pattern of injurious changes present in the rejecting allografts was similar to that observed in certain models of delayed-type hypersensitivity and of skin graft rejection, raising the possibility that lymphokine-mediated mechanisms may be of major pathogenetic significance in this setting.

Animals↗
PubMedDate not supplied

Healthcare restructuring: the impact of job change.

Restructuring, particularly redeployment and job change, had a dramatic impact on the working conditions and practices of nursing personnel. This study was conducted to determine whether nurses (RNs and RPNs) who experienced job change perceived their work-lives differently than those who did not undergo job change and, whether nurses who experienced different types of job change (new role, new unit, or new hospital) varied in their perceptions. A questionnaire exploring themes relevant to redeployment was administered to all nurses (N = 3,408) in two large teaching hospitals that had undergone restructuring. The response rate was 50.7% (n = 1,728). Of the responses, 1,662 were used in the analysis. T-tests and ANOVAs were used to compare groups of nurses. Nurses who changed their jobs perceived their commitment to the organization, their work environment and quality of care differently than those who did not change jobs. Nurses with different types of job change differed in their organizational commitment, perceptions of work-related injuries, attitudes towards job change, need for orientation and new knowledge, and feelings about the health care team. Results will assist managers to address the specific needs of nurses with different experiences of job change in the restructured workplace.

Adult↗
PubMedDate not supplied

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