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J Hirota

Publications and source records attributed to J Hirota.

83 records · Page 5Linked to original sources
PubMed1989

Destruction of basement membrane and cell infiltrates in oral lichen planus.

Immunohistochemical staining analysis of oral lichen planus (OLP) using monoclonal antibodies (MoAbs) against the components of basement membrane (BM) and infiltrated cells was performed in 27 cases. Integrity of lining epithelial BM was more strictly elucidated by staining for laminin and type IV collagen than by fibronectin. Staining patterns of laminin were similar to type IV collagen. In the cases examined, continuity of BM was proven by laminin staining in 10 cases, partially destructive BM was seen in 9 cases and complete destruction of BM in 8 cases. Dividing OLP into 20 symptomatic (ulcerative/erosive) and 7 asymptomatic (non-ulcerative/-erosive) cases, intact BM could be observed in only 5 cases of the symptomatic group, while 5 cases of the other group possessed undamaged BM. The difference between the two groups was significant. Stromal and epidermal cell infiltrates, which were mainly composed of T cell subsets with accompanying Leu-M5 and 6-positive cells, were correlated with the condition of BM. Stromal Leu-M5+ and Leu-6+ cell infiltrates were clearly more dominant in laminin-negative (BM-destructed) cases than infiltrates in BM-intact cases. This relation was also found in epidermal infiltrates with the same manner of Leu-4 infiltration. From the above, it seems that the integrity of BM is concerned with cell infiltrates and clinical manifestations of OLP.

Basement Membrane↗
PubMed1989

Immunohistochemical and histologic study of cervical lymphoepithelial cysts.

Four cases of lateral cervical cysts were reported. All cyst walls were lined with keratinized stratified squamous epithelium, and contained lymph follicles with well-defined germinal centers. Ectopic salivary gland tissue was found in one case. Histochemical examination by the immunoperoxidase method revealed the existence of carcinoembryonic antigen (CEA). However, alpha-feto protein (AFP) and amylase were not detected in any of the cysts. The cyst fluid contained high levels of CEA and high titers of salivary type amylase, but AFP was negative. These results do not support any one of the theories concerning the etiology of cervical lymphoepithelial cysts. It seems likely that cysts derive from both epithelial remnants of the branchial apparatus and from salivary gland inclusions in lymph nodes.

Adolescent↗
PubMed1987

Clinico-pathologic studies on post-operative maxillary cysts.

Microscopic and clinical observations on 70 post-operative maxillary cysts were performed. Cysts of 47 cases were lined with the ciliatal epithelium, and 12 cases were composed of both squamous and ciliated cells. Squamous only and cuboidal lining were found in 9 and 2 cases, respectively. Duration after the antrum operation and the non-vital tooth related to the cyst did not affect the epithelial type, though many of the cases of squamous epithelium possessed infectious histories. From the results, the squamous change seemed to be accelerated by inflammatory irritations, and mucous cells in the lining epithelium were depleted as a part of the metaplastic sequence.

Epithelium↗
PubMedDate not supplied

Immunohistologic study of mononuclear cell infiltrates in oral squamous cell carcinomas.

Intensity of mononuclear cell infiltrates in 50 oral cancer tissues was evaluated by immunohistochemical stainings. The intensity of the infiltrates was graded into four degrees from marked ( ) to absent (-); and the degree of each infiltrated lymphocyte subpopulation including Leu-3a3b/Leu-2a ratio was matched against clinical and histopathologic features. The vast majority of the infiltrated lymphocytes had antigens which were reactive with anti-T cell antibodies. In T cell subsets, Leu-3a3b+ cells infiltrated clearly (grade ++ or ) in 44 cases, and infiltration greater than grade ++ of Leu-2a+ cells was found in nearly half of the cases (26 of 50). In contrast, B cells (Leu-14+ cells) were obviously detectable in only a few cases, and moderate or marked infiltration of the natural killer (NK) population (Leu-11b+ cells) was not observed. Regarding correlation of T cell infiltrates with clinicopathologic features of the tumors, the degrees of Leu-4+ cell and Leu-2a+ cell infiltration became weak with advancing T stage. Leu-2a+ cells also infiltrated weakly into diffusely invading tumors. Out of 28 cases with no metastasis, 22 cases (78.6%) showed grade ++ approximately of Leu-2a+ cells, and only 4 (18.2%) of 22 cases with lymph node metastasis were clearly positive for Leu-2a+ cells (p less than 0.001). However, Leu-3a3b+ cell infiltration was only slightly related to T stage, metastasis, and to mode of invasion. Therefore, the Leu-3a3b/Leu-2a ratio increased significantly in advanced (T3 + T4) tumors, metastasized cases, or diffuse invasion type.

Antibodies, Monoclonal↗
PubMedDate not supplied

Basement membrane type IV collagen in oral squamous cell carcinoma.

Biopsied specimens of 82 oral squamous cell carcinomas were immunohistochemically studied for detection of type IV collagen existence in the basement membrane (BM). Immunoreactivity on BM was semiquantitatively evaluated as limited (+/-), moderate (+), or extensive (+ +), and matched against clinical and histopathologic features. No clear difference in BM deposition could be found in the tumor sites. The amount of type IV collagen decreased in advanced T stage (T3 and T4) tumors. BM deposition was significantly correlated with lymph node metastasis. In 54 N-, 32 had extensive deposition, 18 had moderate, and the remaining had limited. Conversely, only 5 of 28 N+ cases showed good type IV collagen immunoreactivity and 14 cases exhibited limited BM deposition. BM existence was also associated with the mode of invasion. BM deposition decreased in order with the grade (i.e., the more limited the BM deposition, the more diffuse the invasion mode). BM deposition was, however, not related with stromal responses, such as cellular infiltrates and fibrosis. We conclude that immunohistochemical examinations of BM deposition on biopsied specimens seem beneficial for cancer treatment.

Basement Membrane↗
PubMedDate not supplied

Distribution of surviving tumor cells after chemoradiotherapy in tongue and floor of mouth carcinomas.

BACKGROUND: Conservative surgery according to the clinical response to induction therapy is applied in breast carcinomas but not yet in head and neck carcinomas. The possibility of conservative surgery after induction therapy for preservation of function was examined. METHODS: Forty-three tongue and 15 floor of mouth carcinomas that had received induction therapy and tumor resection were submitted for a comparative investigation on the clinical effect of induction therapy and cancer cell degeneration, which was examined in semiserial specimens from the bread-loaf sections of the excised materials, and a classification of the surviving tumor cell distribution in the tissue was proposed. The distribution was divided into four patterns according to the dispersion and volume of the remaining tumor cells (pattern I, no surviving tumor cells; pattern IIA, viable tumor cells in the central superficial region of the original tumor tissue; pattern IIB, viable cells in the superficial portion, but distributed widely; pattern III, massive tumor cell nests in the deep portion, but centrally localized; pattern IVA, sporadical viable cell islands, but not in the periphery; and pattern IVB, diffuse viable cells scattered throughout the original tumor tissue). RESULTS: The original tumor size did not affect the remaining viable tumor cell distribution pattern, but sporadic distribution patterns (patterns IVA and IVB) were observed in most of the T4 cases. In many cases, especially in tumors of the nondiffuse invasion type (invasion of grade 1 and 2), the tumor remission rate was inversely correlated with the dispersion of the viable tumor cells. However, some cases of grade 3 and 4 invasion exhibited sporadical surviving tumor cells even though a high tumor remission rate was obtained. These tumors, especially those located on the floor of the mouth, recurred with high frequency. CONCLUSIONS: Conservative surgery can be applied to nondiffuse invasion cases, according to the tumor remission rate. In T3 and T4 cases exhibiting grade 3 or 4 invasion, conservative surgery is ill-advised, even if more than 90% tumor remission is obtained.

Carcinoma↗
PubMedDate not supplied

Prophylaxis of oral mucositis associated with chemoradiotherapy for oral carcinoma by Azelastine hydrochloride (Azelastine) with other antioxidants.

BACKGROUND: One of the dose-limiting adverse effects of chemoradiotherapy is mucositis, especially oral mucositis. Prophylaxis of severe mucosal reaction would allow application of aggressive chemoradiotherapy to malignancies. METHODS: Sixty-three patients who received inductive concomitant chemoradiotherapy with cobalt 60 (60Co, approximately 30 Gy), peplomycin (PLM, approximately 38 mg), and 5-fluorouracil (5-FU, approximately 3,500 mg) were included in this study. From the start of therapy to the disappearance of oral erosion, 37 patients received daily doses of Azelastine (2 mg) + vitamin C (500 mg) + vitamin E (200) + glutathione (200 mg) (azelastine group), whereas the other 26 patients received the same regimen without azelastine (control group). The severity of oral mucositis in both groups was evaluated periodically. RESULTS: At 10 Gy with 15 mg PLM and 1,250 mg 5-FU, grade 1 mucositis (redness of the oral mucosa) was induced in 14 patients in the control group and five patients in the Azelastine group. At 20 Gy with 30 mg PLM and 2,500 mg 5-FU, grade 2 (erosion with mild irritation) and grade 3 (extensive erosion with marked irritation) stomatitis were observed in 9 and 3 of the control patients and 5 and 1 in the Azelastine group, respectively. At the completion of treatment, mucositis in 21 patients in the Azelastine group remained at grade 1 or grade 2, whereas grades 3 and 4 (ulceration with severe contact pain) mucositis were observed in 6 and 10 patients, respectively. However, in the control group, grades 1 and 2 were observed in only 2 and 3 cases, whereas grades 3 and 4 stomatitis were induced in 6 and 15, respectively. Azelastine suppressed neutrophil respiratory burst both in vivo and in vitro, and also suppressed cytokine release from lymphocytes. However, neutrophil superoxide dismutase (SOD) activity was negligibly suppressed. CONCLUSION: A regimen including Azelastine, which suppresses reactive oxygen production and stabilizes cell membranes, may be useful for the prophylaxis of mucositis due to chemoradiotherapy.

Aged↗