Biomedical subjects

J Hiss

Publications and source records attributed to J Hiss.

At least 19 recordsLinked to original sources

PubMed1998

Biological behavior and cell properties of new AKR lymphoma malignancy variants.

The AKR lymphoma-leukemia is a T lymphocyte neoplasm, most suitable as a model for human T cell malignancies. We have been interested in the process of tumor progression in the AKR lymphoma system. In the present study, two newly isolated variants, the TAU-42 and TAU-44, were characterized with respect to their biological behavior, by comparing them to a previously studied low-malignancy variant, the TAU-39. While the TAU-44 variant formed large s.c. local tumors, the TAU-42 variant formed only small growths or none at all. The TAU-42 lymphoma was found to have the highest malignant potential: it displayed very marked dissemination to spleen, lymph nodes, liver and lungs. The TAU-44 variant had an intermediate degree of metastatic potential but presented a predilection for spread to lymph nodes and spleen and was sometimes found to metastasize to peculiar organs, such as heart and pancreas. Cells derived from the different lymphoma variants varied in their immunophenotype: the highly malignant variant cells expressed more CD4 antigen than the low-malignancy one. The opposite was observed with regard to CD8. The variant cells also differed in their migrating capacity, the more malignant one exhibiting a higher motile activity. Studies on the tumor progression model of AKR lymphoma might contribute to the elucidation of the features determining the aggressiveness of T lymphocytic malignancies.

Animals↗

PubMed1998

Suicide bombers in Israel.

Between 1993 and 1995, 14 suicidal terrorist bombings took place in Israel; 86 victims perished in these attacks, which were carried out by militant Palestinian organizations that oppose peace treaties between the state of Israel and the Palestinian people. The modus operandi of the perpetrators was detonating, in a public area, an explosive device carried on or in close proximity to the terrorist's body. We reviewed the postmortem examinations and identification procedures performed by the medical and law enforcement personnel involved in mass disaster management. The types of injuries sustained by the victims and perpetrators include body disruption, explosive injuries, flying missile injuries, and blast injuries. Blunt trauma directly produced by the explosion and flying missile injuries account for 80.1% of the wounds. The number of fatalities was more closely related to the type of the attack rather than to the amount and type of explosives used. Swift identification of all victims and perpetrators was obtained through collaboration between the different professional teams involved: forensic scientists, law enforcement agencies, and secret service investigators. Based on the analysis of the data obtained from the necroscopic examinations, we observed that most of the wounds sustained fall within the realm of blunt force injuries; emergency medical facilities that might be faced with similar situations should prepare accordingly. Collaboration between the various forensic and law enforcement teams results in swift resolution of disaster management. Prompt identification of the perpetrators allows the authorities to apprehend any accomplices and to prevent similar attacks.

Adult↗

PubMed1998

Quantitative assessment of trabecular bone pattern identification.

The results of the research described in this paper demonstrate that the trabecular architecture is unique to each individual and stable enough to be used as a forensic marker for positive identification of human remains. The trabecular bone architecture depicted on radiographs is often used as an individualizing forensic marker for positive identification of human remains. The aim of the present study was to ascertain the reliability of the trabecular pattern in forensic identification. The trabecular pattern is potentially the best radiographic forensic marker since its presence on a radiograph doesn't depend on a previous pathology or traumatic event. A sample of 305 radiographs of the left wrist of 103 postmenopausal women was studied using an image analyzer. The uniqueness and stability over time of the trabecular architecture was examined by creating line maps or "densitographs" of the ultra-distal point of the radius of each roentgenogram. Pearson's correlation coefficients were calculated for all possible combinations of pairs of radiographs. The correlation coefficient of pairs of radiographs of the same individual, taken at different times (2 to 6 years apart), was always higher than 0.72, while the correlation coefficients of radiographs of different individuals was always below 0.62.

Adult↗

PubMed1997

Radiographic identification of fragmentary human remains from a mass disaster.

Positive identification of human remains is one of the most important tasks in mass disaster management. Here we report on the use of radiography for positive identification of fragmentary human remains recovered from the scene of a terrorist bombing in the Jewish-Argentine Mutual Association Center in Buenos Aires, Argentina, in July 1994. Radiographic examination of all human remains from mass disaster scenes is recommended for identification purposes. Establishing a computerized data bank of antemortem information on missing persons and postmortem findings in disaster victims greatly facilitates and expedites the identification process.

Aged↗

PubMed1997

Forensic medicine in Israel.

We discuss the Israeli medicolegal system, which is rooted in remnants of British jurisprudence. Forensic services for the entire country and the occupied territories (Judea and Samaria), and now the Palestinian Autonomy (Gaza Strip and Jericho), are supplied by one central institute located in Tel Aviv. That organization, the L. Greenberg Institute of Forensic Medicine, is a department of the Ministry of Health and has academic affiliation with the University of Tel Aviv. About 2,000 necroscopic examinations are performed annually, and the types of cases encountered in the forensic practices in Israel are noted. The academic and research activities of the Institute's staff are described, with emphasis on collaboration with overseas forensic scientists. The modern history of the country is reflected in the chronicle of the evolution of its forensic medicine.

Autopsy↗

PubMed1997

Myocarditis misdiagnosed as sudden infant death syndrome (SIDS).

In a retrospective study spanning five years, the histologic sections of 35 autopsies of infants diagnosed as SIDS victims were reviewed. Based on a recently reported study in which findings on marked basement membrane thickening (BMT) in the true vocal cords was suggested as a pathognomonic marker of SIDS, we expected to find BMT in all these cases. However, in seven of the reviewed autopsies (20%) no BMT was detected. Examination of new histologic sections of all the victims revealed myocarditis in these seven cases. In a control group (n = 18) of children with known cause of death, neither BMT nor myocarditis were found. The incidence of myocardial diseases in infants and young adults (20% and 22% respectively) reported in the literature indicates that myocarditis is not a rare cause of sudden death in infants. Therefore, in SIDS-suspected cases a meticulous post-mortem microscopic examination of the heart should be carried out, especially whenever BMT of the vocal cords is absent.

Basement Membrane↗

PubMed1997

Rubber and plastic ammunition lethal injuries: the Israeli experience.

Rubber and plastic ammunition was used by the Israeli Defence Forces between the years 1987 and 1993 (Intifada) almost exclusively as a deterrent to aggression by the Palestinian civilian population in the occupied territories of Gaza, Judea and Samaria. While designed to avoid the serious wounds and deaths caused by the use of conventional military ammunition, the firing of rubber and plastic bullets has resulted in extensive injuries and more than 20 deaths. The data from the autopsies of 17 of these victims, mostly teenagers, were analysed. Ten of the victims died from injuries inflicted by Improved Rubber Bullets, while the other seven succumbed to injuries caused by Plastic Bullets. In most instances, the cause of death was related to injuries to the brain. Ballistic features of non-conventional bullets are discussed, and suggestions to curtail the serious injuries and fatalities arising from their use are given, with emphasis on firing from a safe range. British and South African experience with this type of ammunition is compared with our observations.

Adolescent↗

PubMed1997

Suicidal terrorist bombings in Israel--identification of human remains.

Positive identification of human remains is one of the most important tasks in mass disaster investigations. Religious and jurisdictional demands in Israel, require the identification process to be completed in the shortest possible time. In the 18 suicidal terrorist bombings that took place in Israel between the years 1993-1996, 127 victims and 19 perpetrators were killed, and their severely fragmented bodies were identified within 24 h. The efficient completion of the identification endeavor was enabled by the implementation of a variety of techniques and the close collaboration in the investigation between the different emergency and forensic agencies. This paper presents the mass disaster identification policy and techniques currently used in Israel. The importance of an interdisciplinary approach for the identification of extremely fragmented human remains from mass disasters and the creation of a central data bank of fingerprints and genetic markers is emphasized.

Adolescent↗

PubMed1996

The Abbreviated Injury Scale. A valuable tool for forensic documentation of trauma.

Anatomic trauma scoring systems are fundamental to trauma research. The Abbreviated Injury Scale (AIS) and its derivative, the Injury Severity Score (ISS), are the most frequently used scales. We assessed the applicability of the AIS and the ISS systems for postmortem forensic documentation of trauma. In a prospective study, all trauma autopsies performed between January 1 and June 30, 1993, were coded according to the AIS and ISS method. All cases were reviewed by a consultant in forensic medicine and a traumatologist. Cases were grouped in three categories according to ISS values: 0-14, 16-66, and 75. These categories represent minor, major, and incompatible-with-life injuries, respectively. All autopsy findings in which ISS was < or = 14 were peer-reviewed to establish mechanism and cause of death. In the 6-month period, 279 trauma-related autopsies were studied. Age at death averaged 37.1 +/- 18.7 (mean +/- SD). Eighty-six percent of the victims were male. Penetrating trauma was the mechanism of injury in 67%. ISS was 0-14 in 19 cases, 16-66 in 150 cases, and 75 in 110 cases. In conclusion, AIS and ISS scoring systems are applicable to trauma forensic documentation. Using these methods for coding postmortem findings may help in establishing a database for trauma research, and this information could constitute a major part of continuous quality improvement of trauma management. Low ISS values may serve as a warning, sometimes indicating preventable death.

Abbreviated Injury Scale↗

PubMed1996

Medicolegal investigation of death in custody: a postmortem procedure for detection of blunt force injuries.

Death in custody frequently raises suspicion of ill-treatment on the part of the custodians. The meticulous postmortem search for evidence of injury is the paramount consideration in objective investigation. Detection and evaluation of hidden subcutaneous hemorrhages facilitates reconstruction of antemortem circumstances. We describe a new postmortem technique for accurate examination of the subcutaneous tissues utilizing a simple X-shaped incision of the back and limbs and reflecting the skin. This technique leaves no noticeable disfigurement.

Cause of Death↗

PubMed1996

Beaten to death: why do they die?

The remains of 53 men that had been beaten to death were examined. Six (11.3%) died of either blood aspiration or intracranial hemorrhage; 15 of the cases (28.3%) succumbed to hypovolemic shock, and 32 of the cases (60.4%) died of fat embolism syndrome (FES). Fractures of long bones were found only in four victims. Fatal FES produced by the mechanical disintegration of adipose tissue that migrates into the bloodstream seems to be a common phenomenon in the fatalities examined in this study. No correlation, however, was found between the presence of long bone fractures and the severity of FES, and no bone marrow emboli were detected on histologic examination of target organs. Scattered subcutaneous hematomata were present in all of the victims, although the volume of extravasated blood could not be calculated from the total surface area of the bruises. Contusions limited to the limbs have the potential of masking fatal volumes of extravasated blood that suffuse the musculature mass. Hypovolemic and neurogenic shock, as well as systemic effects of crush injury, should be considered contributing factors to death from FES. These findings contradict the previously published postulate that victims of blunt force trauma succumb primarily to massive hemorrhages.

Adolescent↗

PubMed1995

Concentration and size distribution of insulin-like growth factor-I in human normal and osteoarthritic synovial fluid and cartilage.

The concentration of free insulin-like growth Factor-I (IGF-I) and its complexes was determined in human normal and osteoarthritic synovial fluids, using ultrafiltration through 20- and 100-kDa membranes, followed by a radioimmunoassay of each fraction. In addition, freshly obtained samples of normal and osteoarthritic cartilage were incubated for several days, at both 4 and 37 degrees C. The incubation media (desorbates) were analyzed the same way as the synovial fluid samples to yield the concentration of IGF-I in cartilage in situ. Our findings are (i) Free IGF-I content is extremely low in both human serum and synovial fluid and there is no significant difference between the two; (ii) The concentration of total IGF-I in normal human synovial fluid is an order of magnitude lower than that in serum due mainly to the decrease in the concentration of the large complex; (iii) Preliminary results show that the total IGF-I in osteoarthritic synovial fluids is twice as high as in normal fluids; (iv) In normal human cartilage the levels of IGF-I in all its forms are very low and are consistent with the expected exclusion of large molecules by the extracellular matrix; (v) By contrast, in osteoarthritic cartilage, the concentrations of all forms of IGF-I are high, probably due to increased permeability of the matrix and binding; (vi) The levels of IGF-I found in normal human cartilage are more than an order of magnitude lower than those which stimulate proteoglycan synthesis in human cartilage in culture, while the IGF-I levels in osteoarthritic cartilage lie in the range in which stimulation does occur.

Adult↗

PubMed1995

Insulin-like growth factor-I and its complexes in normal human articular cartilage: studies of partition and diffusion.

Insulin-like growth factor-I (IGF-I) plays a major role in cartilage homeostasis. Our objective was to study the penetration of IGF-I, both alone and bound to serum proteins, into the different zones of normal human cartilage using radioactively labeled IGF-I. The uptake of free IGF-I was higher than that predicted on the basis of excluded volume calculations and showed concentration dependence: we attributed this to reversible binding of the hormone to the tissue. Since the extent of binding was much higher than that calculated for binding to cell receptors, we concluded that IGF-I binds to matrix components. The kinetics of desorption of IGF-I from cartilage confirmed our conclusions regarding binding. The degree of uptake of IGF-I protein complexes prepared by labeling human serum with [125I]IGF-I showed that such complexes are largely excluded from normal cartilage and that the amounts present in the tissue are too low to affect proteoglycan metabolism.

Absorption↗

PubMed1995

Role of immune response as determinant of tumor progression in function of host age in the B16 melanoma.

Aging constitutes the major cause for the development of most neoplastic diseases. However, tumors in aged people present with a lower degree of aggressiveness than in young patients. The reasons for this paradoxical behavior are not clear. We attempted to verify whether the immune system has a role in the relation between host age, immune response and tumor progression. We compared the growth rate of B16 melanoma and a highly malignant variant, the B16/Col/R, in young and aged mice that have or have not undergone splenectomy. The following results were obtained: (1) Splenectomy stimulated growth in the parental melanoma in both young and aged mice, indicating a protective role of the spleen against this tumor at all ages; (2) Spleen ablation provoked inhibition of the highly-metastatic variant growth in young mice, suggesting a stimulatory role of the spleen in this case; (3) By contrast, in aged mice inoculated with the B16/Col/R variant, splenectomy enhanced tumor growth, indicating a defensive role of the spleen. Age favors a positive host response against the aggressive clone of the melanoma. Differential host response in young versus aged mice can explain, in this tumor system, the difference in tumor progression rate as a function of age.

Age Factors↗

PubMed1995

Endoscopic autopsy.

In cases in which the family of the deceased objects to the performance of a conventional autopsy for religious or other reasons, or where there are no forensic pathology facilities in the vicinity of the hospital, postmortem endoscopic examination may be an advantageous and cost-effective substitute for conventional necropsy, especially when the alternative is no postmortem examination at all. To test the reliability of postmortem endoscopy, conventional and endoscopic autopsies were performed on 20 cadavers at the L. Greenberg Institute of Forensic Medicine in Israel. Comparison of the findings of the two procedures showed a very high correlation (100%) for intraperitoneal and thoracic hemorrhages and hepatic, splenic, and diaphragmatic injuries; it showed a slightly lower correlation (60-80%) for mesenteric and retroperitoneal hematomas injuries to the great vessels, blood aspiration, and lung injury. Endoscopy failed to reveal the correct site of injury in the retroperitoneum and posterior aspect of the mediastinum. Collection of body fluids and tissue samples was possible by means of laparoscopy. The technique proved to be relatively accurate, more rapid than conventional autopsy, and left the body virtually intact.

Autopsy↗

PubMed1995

The medicolegal implications of bilateral cranial fractures in infants.

There is considerable debate about the possibility of serious injuries associated with skull fractures caused by short-height falls in early childhood. In order to elucidate the mechanism and nature of blunt head trauma from such falls, we have investigated the possible correlation between patterns of skull fractures and the dynamics inducing these injuries. In a 10-year survey of pediatric medicolegal autopsies conducted at the L. Greenberg Institute of Forensic Medicine in Tel Aviv, Israel, we have observed that all victims of alleged falls from low heights exhibited unilateral skull fractures. Only in four instances were bilateral temporoparietal fractures detected. Thorough investigation into the circumstances surrounding these four incidents, along with the evaluation of the forces exerted upon the cranium at the time of impact, suggest that symmetrical cranial fractures in infants are most likely the result of bilateral compression of the head between two surfaces, rather than the result of a localized impact.

Accidental Falls↗

PubMed1994

Suicidal asphyxia by gas mask.

Antichemical warfare kits that were issued to all Israeli residents 6 months before the Gulf War included masks of various types suited to the age of the user. During the war, 10 adults died of suffocation due to mishandling of the filter of the masks despite the offil Defense Authority's repeated instructions to be cautious. These masks remained in the possession of the population after the war. We report on six cases of suicidal asphyxia incurred by donning the gas mask tightly sealed that have occurred since 1991.

Adult↗

PubMed1994

Utility of postmortem computed tomography in trauma victims.

A possible way to circumvent the continuing decline in the number of autopsies is to perform computed tomography after death. The present study compares the pathologic findings of postmortem CT tomography (PMCT) in trauma fatalities with those disclosed upon conventional forensic autopsy. Within 6 hours of death, the bodies of 25 trauma victims underwent total body CT scanning, all with permission of the relatives, followed by conventional autopsy in 13 cases under court order. The pathologist and roentgenologist were unaware of each other's findings. The pathologic findings of PMCT plus conventional autopsy provided more information than either examination alone. Of the total 127 pathologic findings, 44.9% were diagnosed by both conventional autopsy and PMCT, 29.9% were not revealed by PMCT, whereas conventional autopsy missed 25.2%, and PMCT detected more bone injuries than did autopsy, whereas the latter was superior to PMCT in discovering soft-tissue pathologic states. In all, PMCT revealed 70.5% and autopsy 74.8% of the pathologic states. Although PMCT was not more effective than conventional autopsy in exposing pathologic entities, it increased the yield of findings when combined with conventional autopsy. Where conventional autopsy is unattainable, PMCT may be effective in shedding light on the pathologic state and mechanism of death in trauma fatalities.

Adult↗