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Biomedical subjects

J Hofman

Publications and source records attributed to J Hofman.

88 records · Page 5Linked to original sources
PubMedDate not supplied

[Effect of calcium channel blockers on histamine liberation from isolated basophils and bronchial reactivity in patients with bronchial asthma].

An effect of verapamil and nifedipine on the cellular histamine release and non-specific reactivity of the bronchi measured with methacholine test was studied. In vitro tests were carried out in basophils isolated from 19 asthmatic patients. Reactivity of the bronchi was investigated in 5 patients. Both verapamil and nifedipine significantly inhibited histamine release from the cells in vitro in all examined concentrations (10(-7)-10(-4) M) while a single dose of these drugs administered to the asthmatic patients did not exert a significant effect on the airway constriction in vivo.

Administration, Inhalation↗
PubMedDate not supplied

[Usefulness of testing histamine release from isolated human basophils in anti-allergic and anti-asthmatic drug assessment].

Histamine release from isolated human basophils test was used to evaluate an activity of: histamine receptors H1 and H2 blockers, agonists of beta-receptors, calcium channel blocking agents, hydrocortisone, and disodium cromoglycate (Intal). The study involved 84 patients hospitalized for the bronchial asthma. Basophils were isolated with Day's technique modified by Shov and Norn. Histamine was measured with Shov's spectrofluorimetric technique. It was found that histamine release from isolated human basophils may be used in both evaluation of the mechanism of action and efficiency of drugs used in allergic diseases therapy.

Asthma↗
PubMedDate not supplied

[Influence of pirenzepine on bronchial reactivity evaluated by the methacholine provocation test in a double-blind study of patients with bronchial asthma].

A double blind study was carried out on the effect of pirenzepine on bronchial reactivity assessed by metacholine provocation. The study was carried out on 19 patients with bronchial asthma. Bronchial provocation tests was performed according to the method of Ryan et al using the DeVilbis 646 nebuliser coupled to the Rosenthal--French dosimeter. Spirometric parameters were assessed using Vitalograph. The results show a significant protective effect of pirenzepine on bronchial spasm induced by metacholine.

Adult↗
PubMedDate not supplied

[Comparative study of the T- and B-cell immunity systems in human and animal embryogenesis].

Data are presented on the spleen and thymus structure, time of appearance of the lymphocytes and their heterogeneity in the liver, thymus, spleen, lymph nodes and blood of human foetuses with hemochorial placenta (3 to 34 weeks) and of the minipigs foetuses with epitheliochorial placenta (32 to 95 days). In both foetuses the first T- and B-lymphocytes are found in liver, T-lymphocytes are then found in thymus and later in spleen and lymph nodes whereas B-lymphocytes are found, after liver, in spleen. Kinetics of T- and B-lymphocytes during embryogenesis is described. Reaction of the minipig lymphocytes to mitogens was demonstrated.

Animals↗
PubMedDate not supplied

[Evaluation of the combined action of muscarinic receptor antagonists and beta receptor agonists on reactivity of basophils isolated from blood of patients with atopic bronchial asthma].

The study aimed at evaluating effects of selective M1 receptors antagonist-pirenzepine-and selected beta-receptor-agonists-orciprenaline or salbutamol, given alone or in combination, on histamine release from basophils isolated from patients with atopic asthma. Histamine concentration in the cells was assayed with spectrofluorimetric technique described by Shor and modified by Scov and Norn, using anti-IgE and metacholine as liberators. It was showed, that pirenzepine inhibits histamine release caused by both anti-IgE and metacholine. In the latter case this inhibitory effect was more significant. Beta-sympathicomimetics acted conversely, significantly inhibiting immunological histamine release. This effect was weaker, if metacholine was used. Combination of these agents increased protective effect of pirenzepine on histamine release induced by metacholine.

Adult↗