Biomedical subjects
Publications and source records attributed to J Horvat.
Rabbit anti-rat brain synaptic vesicle serum reacted with thymocytes and B lymphocytes in cytotoxicity and immunofluorescence assays. Quantitative absorption analysis revealed that this antiserum contained antibodies specific for antigenic determinants on the surface membrane of a subpopulation of rat bone marrow B lymphocytes.
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By using rabbit anti-rat thymocyte and anti-rat superior cervical ganglion sera in cytotoxicity, immunofluorescence and absorption assays, it has been shown that surface membranes of rat thymocytes and cervical ganglion cells (i.e. peripheral nervous tissue cells) contain common antigenic determinants.
Patients with cerebral atrophy of unknown origin, patients with nuclear forms of schizophrenia or neurosis, and normal subjects were skin-tested with human brain and liver proteins. The frequency of positive delayed skin-sensitivity reactions to brain proteins was significantly higher in the cerebral-atrophy group than in other groups, thus suggesting a correlation between cerebral atrophy and cell-mediated immunity.
Rabbit anti-human fibrin globulin (A.F.G.) was labelled with iodine (131I) and used as a thrombus-detecting agent. 131I-A.F.G. labelled thrombi were displayed by means of a gamma scintillation camera. Normal subjects and patients with thrombophlebitis of legs, acute fibrin depositions other than thrombi, and chronic varicosities were examined. The 131I-A.F.G. technique detected both formed thrombi and those that were forming and could discriminate between acute thrombosis and chronic varicosities. Thrombophlebitis and extravascular fibrin depositions were best demonstrated between 24 and 27 hours of 131I-A.F.G. injection. Radiolabelled A.F.G. in normal veins and chronic varicosities was best displayed within 6 hours of injection.
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Rabbit anti-rat brain synaptic membrane and anti-rat thymocyte antisera were used in cytotoxicity, immunofluorescence, and absorption assays to define the antigenic relationship between the rat brain and thymocytes, and the localizing properties of antibodies. Both antisera cross-reacted with brain tissue and thymocytes. However, anti-synaptic membrane and anti-thymocyte antisera also contained antibodies specific for neurons and thymocytes, respectively. Immunofluorescence showed that antibodies from anti-thymocyte antiserum reacted with antigenic determinants situated on the surface membrane of brain cells.
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Rabbit antisera to chicken whole brain and brain microsomes, synaptic membranes, and synaptic vesicles were used in cytotoxicity and immunofluorescence assays against chicken bursacytes and thymocytes. Brain organelles, bursacytes and thymocytes have shown to possess common antigenic determinants. The common antigen is present in small amounts on thymocytes and can be demonstrated only by immunofluorescence.
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Explore the source record for details and available documents.