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Biomedical subjects

J Hoyle

Publications and source records attributed to J Hoyle.

13 recordsLinked to original sources
PubMed1997

Localization of genes encoding two human one-domain members of the AAA family: PSMC5 (the thyroid hormone receptor-interacting protein, TRIP1) and PSMC3 (the Tat-binding protein, TBP1).

The eukaryotic genome contains a putative AT-Pase gene family that encodes proteins with one or two highly conserved domain(s) of approximately 230 amino acids. These proteins have diverse cellular functions and mutation in at least one member of the family has been associated with human disease, while mutations in other family members are known to cause cell cycle defects in yeast. Therefore it is of interest to map more family members and so we have localized PSMC5 (the thyroid hormone receptor-interacting protein, TRIP1) and PSMC3 (the Tat-binding protein, TBP1) to chromosomes 17q24-q25 and 11p12-p13, respectively. We also present the map position of a probable PSMC3 processed pseudogene locus on chromosome 9p.

ATPases Associated with Diverse Cellular Activitie↗
PubMed1996

Genomic organization and mapping of the mouse P26s4 ATPase gene: a member of the remarkably conserved AAA gene family.

The eukaryotic genome contains a large family of ATPases in which each member has at least one highly conserved domain of approximately 200 amino acids with an ATP binding motif (the "AAA" domain). AAA ATPases play diverse roles in the cell and are of considerable interest to researchers investigating a number of different phenomena, including control of the cell cycle. We have characterized the mouse P26s4 AAA ATPase gene that encodes a subunit of the 26S protease, a multimeric complex that is responsible for the ubiquitin- and ATP-dependent degradation of specific proteins. The normal functioning of eukaryotic cells depends on this pathway to remove regulatory proteins such as cyclins or signal transduction molecules from the intracellular environment, with the appropriate timing to allow normal cell division and development. We have isolated mouse P26s4 cDNAs and mapped the P26s4 gene to chromosome 12. We have analyzed the intron-exon structure of the P26s4 genomic locus and have determined that the gene contains at least 10 introns, the first of which separates the start methionine from the rest of the coding sequence.

Adenosine Triphosphatases↗
PubMed1996

Characterisation of a short interspersed repeat (Mermaid) that has family members on human chromosome 21 and elsewhere in the human genome.

To understand the architecture of the human genome, we need a complete definition of all the repeat sequence families, as these make up the majority of human DNA. We have isolated a small DNA fragment from human chromosome 21 and have used sequence analysis of this fragment to uncover a new low copy repeat element of approximately 300 bp that we term the Mermaid repeat. This repeat is related to, but is different from, the MER12 repeat and is interspersed in the genome. Mermaid family members that we have studied are between 81%-87% identical to our preliminary consensus sequence. Therefore, we have added a new member to the large collection of human repetitive elements. In addition, we have mapped a Mermaid repeat to a telomeric position on the long arm of human chromosome 21, at 21q22.3.

Base Sequence↗
PubMed1990

Differential expression of two immediate early genes of herpesvirus saimiri as detected by in situ hybridization.

Transcripts from two immediate early (IE) genes have been identified in cells infected with the gamma herpesvirus, herpesvirus saimiri. One is a 1.3 kb RNA transcribed from the HindIII-G fragment of virus DNA (IE-G), the other is a 1.6 kb RNA from the gene for the IE 52K phosphoprotein. Labelled oligonucleotide probes specific for each of these RNAs have been used in in situ hybridization experiments to compare their expression in individual cells in infected populations. In the presence of cycloheximide, the IE-G RNA accumulates synchronously throughout the population of infected cells and prior to the asynchronous accumulation of RNA from the gene for the IE 52K protein in the same population of cells. This heterogeneity in the timing of expression of RNA from the IE 52K gene is paralleled by the asynchronous accumulation of the protein product. We conclude that transcription of the IE-G RNA is independent of expression of the IE 52K gene and that expression of the 52K gene requires (or is prevented by) factors which do not affect accumulation of the RNA from the IE-G gene.

Animals↗
PubMed1989

Prevalence of cytomegalovirus antibody in subjects between the ages of 6 and 22 years.

We determined the prevalence of antibody to cytomegalovirus (CMV) in three groups between 1985 and 1987. Group I consisted of 511 subjects 6-22 y old, group II consisted of 920 subjects 18-21 y old, and group III of 113 subjects 18-22 y old. The overall prevalence of antibody in these three groups was 34%, 24%, and 28%, respectively. Prevalence of antibody in white subjects (24%, 21%, and 24%, respectively) was significantly lower than that in nonwhite subjects. In group I, there was no increase in prevalence with age in white subjects, but the percentage of individuals with antibody increased with age among nonwhite subjects. It is of obvious concern that a large proportion of white women entering childbearing years lack CMV antibody.

Adolescent↗
PubMed1987

Glycerol 1,2-cyclic phosphate in centric diatoms. Observation by 31P NMR in vivo, isolation, and structural determination.

We have isolated and identified glycerol 1,2-cyclic phosphate as the compound responsible for a unique prominent resonance at 19.1 ppm in the 31P NMR spectrum in vivo of four species of centric diatoms, where it is responsible for 15 to 30% of the total signals from organophosphates. This appears to be the first observation of this compound as a major metabolite. It was not detectable in nine other species of algae, including four species of pennate diatoms.

Eukaryota↗