PubMed Health⌕ Search

Biomedical subjects

J Huang

Publications and source records attributed to J Huang.

At least 667 records · Page 37Linked to original sources
PubMed1995

[Observation of immune effect of 5 kinds of Denmark II lyophilized vaccines].

This paper reported five kinds of Denmark II lyophilized vaccines produced by 5 different biological production institutes. Through the double blind randomized trial in newborns after 1 year observation, the results demonstrated that all vaccines possessed the stability of heat tolerance and the 12 week tuberculin conversion rates of all group exceeded to 95%, and 1-year positive maintenance rates were in the range of 88.3%-96.1%, and the average diameter of induration was relatively large; the local reaction belonged to normal range; the swollen-lymph node rate was not high; the immune effect was satisfactory.

BCG Vaccine↗
PubMed1995

A spectrophotometer analysis of light absorption in the human meniscus.

The spectrophotometer analysis of light absorption curves in human meniscal tissue showed a reproducible series of graphs for varying tissue thickness, age, zone, and sectioning plane. These data show water as the essential reason for light absorption in the infrared portion of the light spectrum. The excimer laser in the ultraviolet region, and wavelengths around 2000 nm in the infrared region, appeared to provide the best wavelength match for laser energy transfer and the cutting or ablating of meniscal tissue. On-going research into clinical applications should focus on these wavelengths.

Absorption↗
PubMed1995

[The effect of extract from dermis on fibroblasts of human skin].

The author has observed that the extract from normal human dermis has obvious inhibitory effects on human skin cultured fibroblasts. The results revealed that dermis contains substances which could inhibit growth of fibroblast and that their presence could explain the lack of activity of the normal dermis close to cicatrization.

Cell Division↗
PubMed1995

[The effects of captopril in arterial proliferative response after intima balloon denudation].

We studied the effects of angiotensin-converting enzyme inhibitor on the response of arterial intima after balloon denudation. Captopril (3 mg.kg-1/d) was administered to 4 miniature pigs after abdominal aortic balloon denudation. As controls, 4 pigs were only denuded abdominal aorta without using captopril. All animals were given high cholesterol diet immediately after abdominal aortic denudation. At the end of the 6th and 10th month, 8 pigs were sacrificed equally in two times. Irregular protrusion of arterial wall was observed in all animals at gross anatomy. In the control group, the atherosclerotic plaque was identified via sudan stains. The neointima proliferative response after arterial denudation was found, but the degree of neointima proliferation at captopril group was significantly decreased as compared to non-captopril group (P < 0.01). Microscopic examination showed smooth muscle cell proliferation of arterial neointima in non-captopril group, while slight connective tissue proliferation in captopril group. Electronmicroscopically endothelial cells showed protrusion and their connective gap was increased in the control group. Many platelets were adhered to the arterial neointima, the smooth muscle cells were mainly of secretary type in the same group. The endothelial cells were normal, smooth muscle cells of the neointima were mainly of contractile type in the captopril group. These results illustrate that angiotensin-converting enzyme inhibitor inhibits the proliferative response after arterial intima denudation, on the other hand, angiotensin-converting enzyme may play an important role in neointima proliferative response after intima injury.

Animals↗
PubMed1995

Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-562.

Using fluorescence in situ hybridization (FISH), we were able to demonstrate 22-24-fold amplification of the bcr/abl fusion gene in the human leukemic cell line K-562. About 60% of the amplified sequences are localized to a large acrocentric marker chromosome, with another 30% clustered on a small acrocentric chromosome. In addition to these two masked Ph chromosomes, the remaining bcr/abl fusion genes are located on a der(2) distal to band q33. G- and C-banding analysis revealed similar unique banding patterns in both masked Ph chromosomes and suggests that amplification occurred by tandem duplication of the bcr/abl fusion site. Because the number of bcr/abl fusion genes may be increasing over time, it is critical that researchers using K-562 cells should be aware of this extensive amplification.

Chromosome Mapping↗
PubMed1995

Transcription and translation of gp600 and receptor-associated protein (RAP) in active Heymann nephritis.

UNLABELLED: Active Heymann nephritis (HN) of rat, an autoimmune glomerular disease, is a model for human membranous glomerulonephropathy. The autoantigen of HN is a glycoprotein of approximately 600 kd that is present in both rat and human kidneys. Another kidney protein of 39-45 kd called receptor-associated protein (RAP) is associated with gp600. In normal kidney very little gp600 and RAP can be detected in glomeruli. This study was undertaken to determine whether the synthesis of gp600 and RAP would increase after development of active HN. Kidneys from normal (n = 5) and active (n = 11) HN rats were studied for expression of gp600 and RAP and their mRNAs by immunofluorescence and in situ hybridization. In all HN kidneys in contrast to normal kidneys both the transcription and translation of gp600 were markedly increased in glomeruli and proximal tubules. Transcription and translation of RAP were also increased but less so than gp600. The site of increased transcription of gp600 and RAP in glomeruli was clearly localized to the visceral glomerular epithelial cells. CONCLUSIONS: This is the first study to show increased transcription of gp600 and RAP in active HN and the first study to identify the visceral glomerular epithelial cell as the cell for the increased transcription.

Animals↗
PubMed1995

[Detection and quantification of lipoprotein (a) in atherosclerotic lesions of human aorta].

Lipoprotein(a) [Lp(a)] represents an independent risk factor for atherosclerotic disease. In the present study, the extent to which Lp(a) accumulated in normal and various degrees of atheromatous lesions and the distribution pattern of Lp(a) in these lesions have been studied in thoracic aorta from the autopsy tissues by means of immunohistochemistry, immunoelectronic microscopy, enzyme-linked immunosorbent assay and computer-assisted image processing. Lp(a) was found to be localized preferentially in lesion areas and had its own distribution pattern in each kind of lesion. Lp(a) associated primarily with extracellular matrix and was detected in only a small number of foamy cells. Apolipoprotein B was also detected, whose staining pattern was always found to be congruent with that of Lp(a). This suggests that the immunoreactivity of apoB is related to the presence of Lp(a) at least in part, since Lp(a) possesses apoB in addition to apo(a). It was concluded that both low density lipoprotein and lipoprotein(a) play an important role in the pathogenesis of atherosclerosis.

Adolescent↗
PubMed1995

Activation of human thymocytes induces the phosphorylation of protein tyrosine phosphatase 1C.

Phosphorylation of docking proteins is essential for signal transduction. In this report we provide evidence that activation of human thymocytes in culture induces the phosphorylation of the protein tyrosine phosphatase 1C (PTP 1C). Thymocytes were activated with Con A, PMA or Con A+PMA. The enzyme is phosphorylated on its serine and threonine residues. Phosphorylation occurs within 5 min and lasts for 24 h. PTP 1C is phosphorylated by PKC in vivo and in vitro; however, phosphopeptide mapping suggests that in addition to PKC other kinases phosphorylate the enzyme. On the maps of tryptic digests of cultured thymocytes more radiolabeled phosphopeptides are visualized than on the maps of digests of PTP 1C phosphorylated with partially purified PKC alpha or beta. Phosphorylation of PTP 1C decreases its activity, whereas dephosphorylation increases its activity, suggesting that phosphorylation of PTP 1C takes part in the regulation of signal transduction.

Cells, Cultured↗
PubMed1995

[The changing of orofacial structure by bite opening with Begg technique].

Cephalometric study of 30 deep-bite cases treated with extraction of four first premolars and Begg stage I appliance shows that the bite opening consists of not only intrusion of the upper and lower incisors but also extrusion of the upper and lower molars, furthermore, the lower incisors depressed more than the upper ones and the lower molars are elevated more than the upper ones. The moving type of teeth, the changing of base bone and soft tissue profile are also discussed in this article.

Adolescent↗
PubMed1995

[Application of the wave-speed theory to analysis of mechanical mechanism for configuration of F-V curve in normal humans].

F-V curves were performed in 572 normal male and female adolescents with DFM-86 flow-volume recorder as well as 262 normal male and female adults with LR-80 flow-volume recorder. The mechanical mechanism for the formation of the configuration in normal F-V curves was analysed with the wave-speed theory. The configurations of F-V curves were classified into 4 types, i.e. the plateau, linear, convex and concave types. The plateau type was characterized by: (1) a fast descent just after the peak; (2) a subsequent slow descending plateau; (3) the second fast descent forming a bump or knee; (4) lastly a slow descending tail, possibly with small bumps. The other 3 types were considered as variations of the plateau type. The mechanical mechanism for the formation of the plateau type was chiefly analysed with the wave-speed theory. Our investigation suggested that previous studies on the F-V curve configuration in models and animals with the wave-speed theory could be extended to the exploration in human beings.

Adolescent↗
PubMed1995

[A study on antimicrobial resistance of coagulase negative staphylococci].

One hundred and eight strains of coagulase negative staphylococci (CNS) isolated from different clinical specimens (sputum, urine, blood, various catheters etc.) were classified into 9 species of CNS with biochemical technique. Staphylococcus epidermidis and staphylococcus saprophyticus accounted for the majority (66%). Drug susceptibility tests revealed that they were highly resistant to penicillin, oxacillin, erythromycin, chloramphenicol, compound sulfamethoxazole, tetracycline, cephazolin and ++ceftazidim but relatively sensitive to vancomycin, amikacin, gentamicin and rifapen. The MIC of oxacillin ranged from 0.125 mg/L to 128 mg/L. The MIC90 was 64 mg/L. It is suggested that vancomycin is the therapy of first choice in methicillin resistant CNS infection.

Anti-Bacterial Agents↗
PubMed1995

[Surgical treatment of obstructive jaundice caused by hepatocellular carcinoma].

Between Jan. 1991 and Dec. 1993, 7 patients with obstructive jaundice caused by hepatocellular carcinoma underwent transcholedochal cancerous embolectomy plus T-tube drainage of the biliary tract, in 5 patients with hepatectomy. Detailed discussion was made an the operative procedure and the operative results. The authors indicated that intensive surgical operation is effective in relieving symptoms and prolonging life-span.

Adult↗
PubMed1995

[Microwave tissue coagulation for bladder carcinoma].

47 patients with bladder carcinoma were treated by microwave tissue coagulation (MTC) through urethra. The total effective rate was 100%. Among 43 patients followed-up, 17 had recurrence of the carcinoma 6-20 months after operation and received another MTC therapy successfully. This kind of treatment is indicated for tiny, multiple, scattered and reccurred tumor. It has advantages of safety, easy operation, fast effect, mild suffering. It is an ideal treatment for the patients who are intolerable or unwilling to accept major operation.

Adult↗
PubMed1995

[Vena iliaca externa thrombosis after open operation of the bladder and prostate].

Since 1986, we have performed total cystectomy or partial cystectomy with ureteroneocystostomy in 64 cases and prostatectomy in 182 cases. 5 cases developed vena iliaca external thrombosis (VIET) or thrombophlebitis postoperatively. Three of them recovered after treatment, and 2 died of pulmonary embolism 7 and 16 days after operations. VIET is a very serious complication. Once develops pulmonary embolism, the patient may die soon. We suggested that patients be encouraged to increase limbs activities, be given medicines of antilipemic and antiatherogenic preoperatively. And during the surgical operation, patients' blood pressure should be kept steady, and hypopietic shock should be avoided as long as possible. The vena iliaca external should never be constricted when a deep hook is used for exposure or gauze is used to stop bleeding. After operation, the patient should be encouraged to exercise on bed or around bed as early as possible, so as to increase venous flow. The veins of lower limbs should be avoided by using infusion. Styptic should not be applied abusively. Infusion of dextranum-40 and orally taking of persantinum may also be used to prevent this kind of complication.

Aged↗
PubMed1995

[Identification of streptavidin].

In this report, streptavidin (SA) was further identified on the basis of our previous researches in SA isolation and purification. 23.2 mg of SA productivity could be obtained after purification by DEAE-52 column from 1 L of culture supernatant. The spectrum scanning of ultraviolet absorption was congruent with that of the Sigma product. 2 bands appeared on the SDS-PAGE electrophoresis of SA. The molecular weight of SA was 72 881 Dalton with PI 6.0; the averaged biotin-binding activity was 18 micrograms/mg. The results confirmed that the methods of isolation and purification of SA developed by authors were reliable for immunological application.

Bacterial Proteins↗
PubMed1995

[Effect of endogenous and inhaled nitric oxide on pulmonary microcirculation].

The sites of action of endogenous and inhaled nitric oxide (NO) were reassured during hypoxic pulmonary vasoconstriction. Lungs of 21 adult cats were perfused in situ with autologous blood in zone-3 conditions. Capillary pressures were measured by the double-occlusion techniques and pressures in arterioles and venules 70-100 microns in diameter were measured by the servo-null micropuncture technique, both during normoxia (FiO2 = 0.3) and during hypoxia (FiO2 = 0.02). The effects of NG-nitro-L-arginine methyl ester (L-NAME, 10 mg/kg), an inhibitor of NO synthase, and of inhaled NO (5-100 ppm) were also measured. The PO2 of the prefusate decreased from 187.6 +/- 5.3 mmHg during normoxia to 25.7 +/- 1.3 mmHg during hypoxia, and further decreased to 20.8 +/- 2.2 mmHg during hypoxia with 50 ppm NO (p < 0.05, compared with hypoxia only). Increases in pulmonary vascular pressure drop in response to hypoxia were 4.8 +/- 1.0 cmH2O and 9.1 +/- 1.4 cmH2O in non-treated and L-NAME-treated lungs, respectively (p < 0.05). L-NAME significantly increased hypoxic construction in the venous segment. The concentration of exhaled NO increased from 13 +/- 4 ppb during normoxia to 18 +/- 4 ppb during hypoxia (p < 0.1). Inhaled NO lowered not only pulmonary artery pressure but also capillary pressure in a dose-dependent manner, which reduced hypoxic pulmonary vasoconstriction. Pulmonary veins were more sensitive to inhaled NO than were arteries. Inhaled NO (50 ppm) dilated vessels smaller than 70 to 100 microns in diameter, by 39% (p < 0.05), and dilated venules greater than 100 microns in diameter by 26% (p < 0.05), but did not significantly dilate arterioles greater than 100 microns in diameter (11%). Inhaled NO did not significantly change the ratio of wet weight to dry weight. We conclude that both endogenous and inhaled NO attenuate hypoxic pulmonary vasoconstriction, with significant pulmonary venous dilation. The main site of action of inhaled NO is vessels smaller than 100 microns in diameter and venules greater than 100 microns in diameter. Inhaled NO (5-100 ppm) does not cause interstitial edema.

Administration, Inhalation↗