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J Hukki

Publications and source records attributed to J Hukki.

26 records · Page 2Linked to original sources
PubMed1989

Comparison of surgical lasers and conventional methods in skin incisions.

Steel scalpel, electrocautery, CO2 laser used in a continuous wave mode (CW) and rapid superpulse mode (RSP), and contact Nd: YAG laser were tested on pig skin incisions. Speed of incision and histological changes near the wounds were examined. Light microscopical observations were made on postoperative day 0 using standard Van Gieson stain. Width of the scar on postoperative day 14 was also measured. Steel scalpel produced the least pathological changes in the skin, followed by RSP. Electrocautery did not differ significantly from the CO2 lasers in this respect on postoperative day 0. The damage was larger after contact Nd:YAG laser. The situation was essentially similar on postoperative day 14. The width of the scar was narrowest after steel scalpel and widest after contact Nd:YAG laser (p less than 0.01; Nd:YAG vs. other methods). Electrocautery and the two CO2 lasers produced equal scarring. However, electrocautery was significantly faster than any of the lasers (p less than 0.001).

Animals↗
PubMed1988

Effects of different contact laser scalpels on skin and subcutaneous fat.

The tissue effects of different Nd:YAG contact laser scalpels on pig skin and subcutaneous fat were studied using various power settings. Three different laser scalpels were tested: 0.2 mm nonfrosted (LR2), 0.6 mm nonfrosted (LR6), and 1.0 mm frosted (LRP10), using 3 different power settings (8 W, 14 W, 18 W). The tissue effects of a steel scalpel were used as reference. Incisions on pig loin (40/pig) were made at randomized sites. Specimens were taken at postoperative days 0 and 14. The depth of tissue damage was measured using a light microscope. The amount of time required for making each incision was recorded. Significant differences between the LR2 and the LR6 were observed in the skin at 2 weeks (P less than .05) at all power settings used, indicating superiority of the smaller scalpel for use in skin incisions. The LRP10 did not cause more tissue damage than did the LR6 in any of the power settings used. In subcutaneous fat the smallest scalpel (LR2) caused significantly less tissue damage evident at 2 weeks postoperatively (P less than .05) than did the other two laser scalpels, whereas the effects of the different power settings were minor. The incision time in skin decreased by more than 50% when the power was increased from 8 W to 18 W for all laser scalpels studied.

Adipose Tissue↗
PubMed1988

Degeneration and regrowth of adrenergic nerves after microvascular anastomosis. A fluorescence histochemical study on end-to-end anastomoses of femoral vessels in the rat.

The normal femoral artery and its branches were found to be innervated with a dense network of adrenergic nerves. The nerve plexus around the vein was sparse. Adventitial stripping of the femoral vessels, with or without division and reanastomosis, caused local disappearance of catecholamine fluorescence in the stripped area. The distal adrenergic innervation remained normal if the femoral nerve was left intact. Division of the femoral nerve, alone or in combination with blood vessel division and reanastomosis, caused total disappearance of catecholamine fluorescence from the adrenergic nerves of the entire distal neurovascular tree examined. At the end of the observation period of 36 weeks from the time of division of the nerve, artery and vein with subsequent microvascular anastomosis, numerous adrenergic nerves were observed to have crossed the suture line. The vascular nerve plexus around the femoral vessels was dense in places, but in other places sparse or absent. It seems that the reinnervation occurs not only over the suture line, but also together with other regenerating nerves from the adjacent tissues and by collateral sprouting from adjacent adrenergically normally innervated areas.

Adrenergic Fibers↗
PubMed1988

Adrenergic innervation of aortic patch-grafts in rats.

The regeneration of vascular adrenergic nerves was studied using the glyoxylic acid-induced fluorescence method for the specific demonstration of adrenergic nerves in syngeneic patch-grafts of the right atrium of the heart, vena cava and glutaraldehyde-treated vena cava transplanted into the abdominal aorta of the rat. Glutaraldehyde-treated segments of the supradiaphragmatic inferior vena cava were transplanted into the abdominal aorta of rats as well. At the end of the observation period of 24 weeks limited, patchy and defective innervation was observed in the syngeneic vena cava and atrial patches. No adrenergic nerves were found in the glutaraldehyde-treated vein patch-grafts or vein grafts. Owing to the very poor innervation of atrial and venous patch-grafts the results are not entirely in agreement with the target organ concept of adrenergic nerve regeneration. In this study the suture line around the patch graft probably hampers regeneration of vascular adrenergic nerves in the patches.

Adrenergic Fibers↗
PubMedDate not supplied

Congruence of adrenergic and cholinergic intrinsic innervation of human and rat atria.

Right atrial biopsies from rat and human hearts were studied using combined methods for the demonstration of glyoxylic acid-induced fluorescence (GIF) of catecholamines and acetylcholinesterase (AChE) reactions in the same specimens. In specimens from the rat heart, the GIF and AChE reactions were performed either simultaneously or consecutively. In biopsies of the human right atrium, obtained at right atria cannulation during open-heart surgery, the reactions were performed consecutively. It was found that in both rat and human atria the adrenergic nerves and AChE reactive nerves, which are probably cholinergic, run partly separately and partly together. In the rat atrial specimens, close relations between adrenergic nerves and clusters of AChE reactive cells were observed. In addition, clusters of fluorescent cells were observed in the vicinity of AChE reactive nerve bundles. This indicates that there may be several possible means of peripheral interaction between the intrinsic adrenergic and cholinergic systems of the rat heart.

Acetylcholinesterase↗
PubMedDate not supplied

Burn excision with contact neodymium: YAG laser.

The newest laser modality, the contact Neodymium: YAG laser, was compared with the standard steel scalpel in excisions of full-thickness burns. Six patients with deep burns of variable sizes were included in this pilot study. The area to be operated on in each patient was divided into two areas resembling each other as much as possible in size, tissue type, and depth of injury. The results suggest that bleeding was diminished by 44% in contact laser surgery compared with the steel scalpel. Still the difference was not significant. There was no impairment of graft take after laser surgery compared with the steel scalpel. However, the laser method caused a significant increase (p less than 0.02) in operating time. The contact Nd: YAG laser can be recommended for excision of relatively small deep burn injuries, especially in areas in which bleeding is a problem with other methods.

Adult↗