PubMed Health⌕ Search

Biomedical subjects

J Hunyadi

Publications and source records attributed to J Hunyadi.

At least 73 records · Page 4Linked to original sources
PubMed1986

Expression of OKM5 antigen on human keratinocytes in vitro upon stimulation with gamma-interferon.

Using murine monoclonal antibodies against human OKM5, OKM1 and HLA-DR antigens antigenic characteristics of freshly separated human epidermal cells (EC) and those of EC cultured in the presence of Interferon-gamma (IFN-gamma) were studied. After 8-12 days of culture, primarily OKM1- OKM5- HLA-DR- keratinocytes displayed OKM5 and HLA-DR antigens when exposed to IFN-gamma. Our data support the concept, that human keratinocytes may possess accessory cell functions.

Antigens, Surface↗
PubMed1984

The effect of mouse erythrocyte rosette forming lymphocytes on lymphokine production in T-cell cultures.

An enhancement effect of mouse erythrocyte rosette forming (MERF) cells on the production of migration inhibitory factor, chemotactic factor for neutrophils and skin reactive factor in T-lymphocyte cultures stimulated with the purified protein derivative of tuberculin was observed. We consider it likely that the MERF cells, possessing the appropriate cell surface constituents to construct an immunogenic moiety, present antigen on their surfaces to elicit lymphokine production.

Animals↗
PubMed1983

A comparative study of the inhibitory effect of trypan blue on mouse erythrocyte and C3 binding receptors of peripheral blood mononuclear cells from healthy donors and patients suffering from chronic lymphocytic leukaemia.

Trypan blue has previously been shown to interact with the C3 receptor, but not with the Fc receptor. In the present work the effects of Trypan blue on mouse erythrocyte (ME) and C3 binding receptors of the peripheral blood mononuclear cells have been studied in healthy individuals and in patients with chronic lymphocytic leukemia (CLL). The resulting dose-response curves have been compared with each another. The results of statistical analysis indicate that there is no significant difference between the effects of trypan blue on the two receptors in healthy individuals. In contrast ME and C3 binding receptors differ significantly in trypan blue sensitivity in CLL patients. These data suggest that the two receptors are similar, or are situated on the cell membrane in such a way that the trypan blue bound to one of them inhibits the functioning of the other one too, but they are not the same. In the course of leukemic transformation, the trypan blue sensitivity of the ME binding receptor does not vary, whereas that of the C3 receptor is enhanced significantly.

Animals↗
PubMed1983

Relationship of mouse erythrocyte binding receptor on peripheral blood mononuclear cells to receptors for IgG Fc and C3.

When peripheral blood mononuclear cells separated at 4 degrees C were incubated at 37 degrees C, they shed their surface receptors. Total absorption of the released mouse erythrocyte binding receptors from the supernatant of the cell suspension with mouse erythrocytes did not lead to a change in the IgG Fc and C3 receptor content of the supernatant. On removal of the C3 or IgG Fc receptors, the quantity of mouse erythrocyte binding receptor remained unchanged. These observations indicate that the three receptors are mutually independent structures, or undergo dissociation in the course of the shedding.

Animals↗
PubMed1982

[Comparative immunologic studies in PUVA and SUP (selected ultraviolet phototherapy) treatments].

10 PUVA and 13 SUP-treated psoriatic patients were examined immunologically. The proportion of sheep and mouse erythrocyte rosette forming lymphocytes, the leucocyte migration inhibitory assay, the lymphocyte transformation test were carried out and the suppressor function of peripheral blood mononuclear cells was measured before and 48 hours after the clearing of lesions. The results observed before and after the treatment were compared to each other and to the values of healthy volunteers. No significant difference was observed between the results obtained before and after the treatment, meaning that the immune alterations, which have been observed by several authors immediately after PUVA therapy or UV light irradiation, were not detectable if the tests wer made 48 hours later than the last treatment. The significantly decreased suppressor function of peripheral blood mononuclear cells was not changed during the therapy either.

Cell Migration Inhibition↗
PubMed1982

Regulation of lymphokine production in peripheral blood mononuclear cell cultures.

An increase in the production of macrophage migration inhibitory factor, chemotactic factor for neutrophils, and skin reactive factor, was observed in lymphocyte cultures if the cells were allowed to age in culture for 24 h. The increased lymphokine production was reduced by adding concanavalin A-stimulated and mitomycin C-treated suppressor cells. It is suggested that the lymphokine production could be regulated by suppressive mononuclear cells.

Cells, Cultured↗
PubMed1981

Polymorphonuclear granulocyte chemotaxis and chemotactic factor generation by concanavalin A-stimulated peripheral blood mononuclear cells in patients with psoriasis.

Polymorphonuclear leukocytes from patients with psoriasis demonstrated a significantly enhanced chemotactic responsiveness to zymosan-activated human serum as compared to granulocytes from healthy volunteers. Furthermore, psoriatic peripheral blood mononuclear cells stimulated with concanavalin a produced an increased amount of lymphocyte derived chemotactic factor (LDCF) as compared to that in the case of healthy persons. The LDCF proved to be chemokinetic for the psoriatic granulocyte. It is postulated that these two phenomena may play a role in the pathogenesis of psoriasis.

Adolescent↗
PubMed1981

Increased cytotoxic sensitivity of YPC-1 tumor cells from mice treated with nitrosoureas.

The relative cytotoxic sensitivity of the YPC-1 tumor target cells from untreated mice and from animals treated with nitrosoureas was determined. The amount of 51Cr released from target cells increased significantly when the cells were obtained from treated mice. On the basis of the results of cold-target cytotoxicity inhibition assay, this enhancement was shown to be haplotype specific. The amount of 51Cr released from target cells of mice treated with N,N'-bis(chloroethyl)-N-nitrosourea decreased significantly when the tumor cells were first incubated with fibrinogen and transglutaminase. Based on these results and other published data, a model system is suggested. The model is based on the observation that tumors, and thus tumor antigens, at the cell surface are partly or completely covered by fibrinogen or fibrin. The enzyme transglutaminase is involved in the binding of the fibrinogen or fibrin to the cell surface. Accordingly, it is hypothesized that the nitrosoureas have a dual mode of immunotherapeutic activity. The carbamoylating properties inhibit the fibrin-binding activity of transglutaminase, thus preventing fibrin from covering up or coating the tumor cells and preventing the ability of sensitized effector cells to recognize the tumor-specific antigens in association with self H-2 antigens. The alkylating property of the nitrosoureas mainly concerns reactivity with the DNA of the tumor cells.

Animals↗
PubMed1980

A comparative study of the shedding process mouse erythrocyte binding receptors on peripheral blood lymphocytes from healthy donors and patients suffering from chronic lymphocytic leukemia.

The shedding process of the mouse erythrocyte binding receptors of human peripheral lymphocytes of a healthy donor group was compared to that of patients with chronic lymphocytic leukemia. The shedding process exhibited significant differences with respect to the groups tested, suggesting that the mouse erythrocyte binding receptors were altered during leukemic transformation or that the leukemic cells were fixed in a stage of maturation.

Animals↗