PubMed Health⌕ Search

Biomedical subjects

J Ito

Publications and source records attributed to J Ito.

At least 343 records · Page 19Linked to original sources
PubMed1983

Characterization and partial purification of neuroblastoma growth inhibitory factor from the culture medium of glioblasts.

Neuroblastoma growth inhibitory factor (NGIF) exists in the conditioned medium of normal rat glioblasts. When neuroblastoma cells (Neuro2a, NS-20Y, and N1E-115) were cultured in the presence of the factor, the cell growth rates and DNA synthesis were markedly inhibited and the morphological differentiation including neural process formation was induced. However, the factor neither altered the growth rate nor the morphology of non-neuronal cells such as glial cell lines (C6 and 354A) or fibroblast (3T3). The molecular weight of the factor was estimated to be 75,000 Mr by gel filtration with Bio-Gel P-200, and the isoelectric point was 5.8. The factor was devoid of esteropeptidase activity, and susceptible to protease and thermal treatment. The growth inhibitory action of the factor was unrelated to the intracellular contents of cyclic AMP and GMP. The ability of NGIF to suppress preferentially the neural growth suggests its regulatory role in normal brain development.

Animals↗
PubMed1983

Vestibular efferent fibers to ampulla of anterior, lateral and posterior semicircular canals in cats.

Origins of vestibular efferent fibers to ampulla of semicircular canals in cats were examined using retrograde transport of horseradish peroxidase. The anterior canal was innervated from bilateral parvocellular reticular nucleus (PCRN), contralateral gigantocellular reticular nucleus and ipsilateral lateral reticular nucleus (LRN); the lateral canal, from ipsilateral PCRN and LRN as well as ipsilateral lateral vestibular nucleus; and the posterior canal, from bilateral PCRN and ipsilateral medial and lateral vestibular nuclei.

Animals↗
PubMed1983

Dural arteriovenous malformations of the base of the anterior cranial fossa.

Two cases of dural arteriovenous malformation at the base of the anterior fossa are reported. The angiographic appearance in both cases is quite uniform and resembles the findings in 12 cases previously reported. Feeders are anterior and/or posterior ethmoidal arteries and the external carotid system also contributes. A drainage vein always shows aneurysmal dilatation of its proximal portion. CT findings are also mentioned and radiological literature reviewed.

Arteriovenous Malformations↗
PubMed1983

Protein-primed initiation of phage phi 29 DNA replication.

We recently reported the development of an in vitro replication system for bacteriophage phi 29 DNA. We have used this system for the isolation of replication activity associated with gene 3 protein (terminal protein) from phi 29-infected Bacillus subtilis cells. We utilized two assay systems: (i) DNA replication dependent on phi 29 DNA with the 5' end covalently linked to terminal protein (DNA-protein) and (ii) the formation of complex between the terminal protein and dAMP. The DNA-replication and the complex-forming activities were purified together through all steps. The complex of terminal protein and dAMP formed in the purified fraction was shown to serve as an effective primer for successive chain elongation in the presence of dNTPs by a pulse-chase experiment. The protein fraction purified from cells infected with a temperature-sensitive phi 29 mutant in gene 3 was thermolabile compared to the wild-type activity in the assay system for complex formation. This shows that the purified fraction having replication activity includes the gene 3 product of phi 29. Both the DNA replication and the complex formation activities are highly specific for phi 29 DNA-protein as template. The product analysis of elongated DNA revealed that the replication starts at both termini of the phi 29 genome. These results are consistent with the basic elements of the protein-priming model for the initiation of linear DNA synthesis.

Bacillus subtilis↗
PubMed1983

Treatment of moyamoya disease by temporal muscle graft 'encephalo-myo-synangiosis'.

10 young patients from 7 to 16 years of age with moyamoya disease were treated by temporal muscle graft (encephalo-myo-synangiosis: EMS) for the purpose of increasing extracranial and intracranial collateral circulation. Ischemic cerebrovascular symptoms and signs were encountered in all of them without intracranial hemorrhage. The patients were observed for 6-26 months after the first operation. Transient ischemic attacks disappeared in 4 out of 7 patients, and improved in frequency and duration in the remaining 3. Paresis of the limbs disappeared in 1 patient and improved in 2. In 2 patients, facial palsy disappeared and dysarthria improved as well. Ataxia disappeared in 2 patients, and involuntary movement of the extremities disappeared in 2. There were no ineffective cases clinically. In postoperative carotid angiography, the middle cerebral arteries were visualized clearly to the peripheral portion mainly via the thickened deep temporal arteries, and the abnormal vascular networks in the region of the basal ganglia reduced in size in most of patients. Improvements in intelligence quotient, electroencephalogram and cerebral blood flow were also obtained in several patients, respectively. EMS seemed to be an effective surgical treatment in young patients with moyamoya disease who suffered from cerebral ischemic symptoms.

Adolescent↗
PubMed1983

Effects of diphenhydramine iontophoretically applied onto neurons in the medial and lateral vestibular nuclei.

Electrophysiological studies were carried out to elucidate the effects of diphenhydramine, an antihistamine (H1-receptor blocking) drug, on neuron activities in the medial vestibular nucleus (MVN) and lateral vestibular nucleus (LVN) of cats using a microiontophoretic method. According to the firing pattern and latency of the first spike with vestibular nerve stimulation, neurons in the MVN and LVN were classified into two groups: monosynaptic and polysynaptic neurons. In the MVN, the spike generation of polysynaptic neurons was dose-dependently inhibited with the iontophoretic application of diphenhydramine up to 200 nA, and that of the monosynaptic neurons was also suppressed by the maximum dose of 200 nA. In contrast to the MVN neurons, the spike generation of LVN monosynaptic neurons remained unaffected with diphenhydramine up to 200 nA, although an inhibition of the LVN polysynaptic neurons was obtained with 200 nA of the drug. These results suggest that small doses of diphenhydramine more selectively interfere with synaptic transmission in the MVN neuron than that in the LVN neuron.

Animals↗
PubMed1983

Myxopapillary ependymoma of the lateral ventricle. A study on the mechanism of its stromal myxoid change.

Reported was the first case of myxopapillary ependymoma arising from the right lateral ventricle of a 29-year-old Japanese male. The histological and ultrastructural findings were identical to those from the filum terminale. It was suggested that insudation of plasma proteins found within hyaline-thickened blood vessels in the tumor stroma represented the presence of previous long-standing anoxia and thus, circulatory disturbance would be closely related to the formation of the stromal myxoid change.

Adult↗