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Biomedical subjects

J J Chang

Publications and source records attributed to J J Chang.

At least 91 records · Page 5Linked to original sources
PubMed1983

Renin: structural features of active enzyme and inactive precursor.

To determine the structural basis for the unique catalytic mechanism of renin and the mechanism of activation of inactive renin, renin and inactive renin were isolated in pure form. The active site of renin consists of two aspartyl residues, two tyrosyl residues, and one arginyl residue, analogous to pepsin and other acid proteases. The complete amino acid sequence of mouse submaxillary gland renin was determined. Of the amino acids, 43% were identical to those in porcine pepsin. Combination of various chromatographic techniques permitted the separation of inactive renin from active renin in human plasma and kidney. Inactive renin of hog kidney was completely purified. Inactive renin consists of a single polypeptide chain and is activated by proteolysis but not by dissociative reagents such as 4 M NaCl or detergent. Thus it was concluded that the inactive renin in these tissues is renin zymogen rather than a renin-inhibitor complex.

Amino Acid Sequence↗
PubMed1982

Amino acid sequence of mouse submaxillary gland renin.

The complete amino acid sequences of the heavy chain and light chain of mouse submaxillary gland renin have been determined. The heavy chain consists of 288 amino acid residues having a Mr of 31,036 calculated from the sequence. The light chain contains 48 amino acid residues with a Mr of 5,458. The sequence of the heavy chain was determined by automated Edman degradations of the cyanogen bromide peptides and tryptic peptides generated after citraconylation, as well as other peptides generated therefrom. The sequence of the light chain was derived from sequence analyses of the peptides generated by cyanogen bromide cleavage or by digestion with Staphylococcus aureus protease. The sequences in the active site regions in renin containing two catalytically essential aspartyl residues 32 and 215 were found identical with those in pepsin, chymosin, and penicillopepsin. Comparison of the amino acid sequence of renin with that of porcine pepsin indicated a 42% sequence identity of the heavy chain with the amino-terminal and middle regions and a 46% identity of the light chain with the carboxyl-terminal region of the porcine pepsin sequence. Residues identical in renin and pepsin are distributed throughout the length of the molecules, suggesting a similarity in their overall structures.

Amino Acid Sequence↗
PubMed1982

Molecular characterization of inactive renin: complete purification of prorenin in hog kidney and isolation of inactive renin from neuroblastoma cells: evidence for 2 different types of inactive renin.

To determine the molecular properties of inactive renin and its relationship to active renin, inactive renin in hog kidney was purified by devising affinity chromatography. Electrophoretically homogeneous inactive renin was prepared by 3 million-fold purification. It consists of a single polypeptide chain and undergoes reduction in molecular weight from 50,000 to 38,000 upon activation by proteases but not by dissociative treatment. This type of inactive renin is considered as a zymogen. However, a stable complex of renin and its inhibitor with a molecular weight of 110,000 was found in cultured neuroblastoma cells indicating the presence of a second type of inactive renin.

Animals↗
PubMed1981

Lack of distant relationship between lysozyme Ch and hen egg white lysozyme: computer comparison studies.

A computer search, made for distant relationships between lysozyme Ch, hen egg white lysozyme, and bacteriophage T4 lysozyme, revealed no unusual similarities in their amino acid sequences. Also, antibodies generated against lysozyme Ch failed to cross react with hen egg white lysozyme and vice versa. These lysozymes most likely represent examples of convergent evolution.

Amino Acid Sequence↗
PubMed1980

Rapid taste-aversion learning by an isolated molluscan central nervous system.

The isolated lips and nervous system of the terrestrial slug Limax maximus will produce some of the feeding behavior of the intact animal; i.e., they generate the rhythmic neural activity characteristic of ingestion in response to food extracts applied to the lips. This preparation will respond to a variety of food extracts that elicit feeding in the whole animal. This provides the opportunity for aversive conditioning experiments involving taste discrimination. Pairing lip chemostimulation by attractive food extracts with lip chemostimulation by using bitte plant secondary substances can cause the isolatd brain to selectively suppress its neural response to one food extract while remaining responsive to another. Such isolated brains can learn after one or two trials and retain the learning for more than 8 hr.

Action Potentials↗
PubMed1979

Symmetry perception and spatial-frequency channels.

It is known that the sum of a random-dot array with vertical bilateral symmetry and one with horizontal bilateral symmetry appears as a random array. Here we show that if the vertically and horizontally symmetrical arrays are spatially filtered, so that their respective spectra are 2 octaves apart, then their superposition does not appear random, but both symmetries can be simultaneously perceived. The low-band array has a stronger perceptual weight than the high-band array. These demonstrations give further evidence that frequency channels are before symmetry perception.

Depth Perception↗
PubMed1978

Feeding motor program in Limax. I. Neuromuscular correlates and control by chemosensory input.

The feeding motor program(FMP) of the terrestrial slug Limax maximus was examined in vivo and in vitro. The feeding pattern of intact animals shows an initial increase in bite frequency followed by a plateau phase. Recordings obtained from semi-intact preparations of the lips, brain, and buccal mass established the correlation of activity in buccal ganglion nerve roots with the protraction-retraction bite cycle. A preparation of the lips, cerebral ganglia, and buccal ganglia was developed, such that, repetitive chemostimulation of the lips yields reproducible bouts of FMP. Sources of proprioceptive feedback from buccal muscles were demonstrated. The feasibility of computer scoring of the FMP is documented. The results demonstrate that aspects of in vivo feeding behavior are retained and identifiable in highly dissected, in vivo preparations.

Animals↗
PubMed1978

Inversion of articulatory-to-acoustic transformation in the vocal tract by a computer-sorting technique.

We present numerical methods for studying the relationship between the shape of the vocal tract and its acoustic output. For a stationary vocal tract, the articulatory-acoustic relationship can be represented as a multidimensional function of a multidimensional argument: y=f(x), where x, y are vectors describing the vocal-tract shape and the resulting acoustic output, respectively. Assuming that y may be computed for any x, we develop a procedure for inverting f(x). Inversion by computer sorting consists of computing y for many values of x and sorting the resulting (y,x) pairs into a convenient order according to y; x for a given y is then obtained by looking up y in the sorted data. Application of this method for determining parameters of an articulatory model corresponding to a given set of formant frequencies is presented. A method is also described for finding articulatory regions (fibers) which map into a single point in the acoustic space. The local nature of f(x) is determined by linearization in a small neighborhood. Larger regions are explored by extending the linear neighborhoods in small steps. This method was applied for the study of compensatory articulation. Sounds produced by various articulations along a fiber were synthesized and were compared by informal listening tests. These tests show that, in many cases of interest, a given sound could be produced by many different vocal-tract shapes.

Acoustics↗