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Biomedical subjects

J J Kearney

Publications and source records attributed to J J Kearney.

12 recordsLinked to original sources
PubMed1991

Tracking the violent criminal offender through DNA typing profiles--a national database system concept.

Implementation of standard methods for the conduct of restriction fragment length polymorphism analysis into the protocols of United States crime laboratories offers an unprecedented opportunity for the establishment of a national computer database system to enable interchange of DNA typing information. The FBI Laboratory, in concert with crime laboratory representatives, has taken the initiative in planning and implementing such a database system. The Combined DNA Index System (CODIS) will be composed of three sub-indices: a statistical database, which will contain frequencies of DNA fragment alleles in various population groups; an investigative database which will enable linkage of violent crimes through a common subject; and a convicted felon database that will serve to maintain DNA typing profiles for comparison to profiles developed from violent crimes where the suspect may be unknown.

Computer Communication Networks↗
PubMed1987

Laser injury to multiple retinal foci.

A 21-year-old man received an accidental laser injury to three separate foci of the retina of his right eye from a Q-switched Nd:YAG laser target designator emitting at 1,064 nm, operating at 10 Hz, with a pulse duration of 20 ns. The nominal output of this device was 50 mJ, and the diameter of the output beam was approximately 4 cm. The extent of injury consisted of retinal necrosis, subretinal hemorrhage, vitreous hemorrhage, and striate retinopathy. The initial visual acuity was 20/400, but quickly recovered to 20/30. One year following injury the visual acuity was 20/40. Mature chorioretinal scars were apparent. A preretinal membrane was evident with marked wrinkling effect on the macular area of the retina.

Accidents, Occupational↗
PubMed1986

A device for keratometry in the supine patient.

We fabricated a device that allows suspension of a keratometer from an operating microscope stand in a manner that permits keratometry to be performed with the patient in the supine position. The specifications for this device are given to allow replication by a hospital machinist.

Cornea↗
PubMed1986

Subtyping phosphoglucomutase-1 in semen stains and bloodstains: a report on the method.

A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.2-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The gel contained 1.2% (w/v) N-(2-hydroxyethyl) piperazine-N-3-propanesulfonic acid (EPPS) and pH 5 to 7 ampholytes (4% w/v). When maintained at room temperature, laboratory-prepared bloodstains and semen stains could be typed for phosphoglucomutase-1 up to four months and three weeks, respectively. An evaluation of phosphoglucomutase-1 typing by isoelectric focusing and the Group I system was performed on casework samples submitted to the FBI Laboratory. In addition to the increased discriminating probability of phosphoglucomutase-1 when subtyped, isoelectric focusing yielded an increase in positive calls on questioned bloodstains (65.6 versus 36.2%) and dried seminal stains (16.4 versus 13.1%) compared with the Group I system.

Blood Stains↗
PubMed1986

A double origin electrophoretic method for the simultaneous separation of adenosine deaminase, adenylate kinase, and carbonic anhydrase II.

A rapid, reliable method for the simultaneous separation of adenosine deaminase, adenylate kinase, and carbonic anhydrase II by agarose gel electrophoresis is presented. This method uses a double origin sample application system. Unreduced sample extracts for adenylate kinase analysis are applied 13.0 cm from the anode. Reduced sample extracts for the remaining proteins of interest are applied 7.0 cm from the anode. The use of applicator foils and an increased voltage gradient result in superior resolution, linearity, and band sharpness of the allozyme patterns. Further, there is no masking of the adenylate kinase 2 band as a result of the use of a reducing agent, and carbonic anhydrase II is resolved without interference from hemoglobin as has been observed with other multisystem methods.

Adenosine Deaminase↗
PubMed1969

Direct determination of serum T-4 by isotopic exchange: comparison with T-3 and PBI in 1700 cases.

We have studied in detail the procedure for the direct determination of serum thyroxine based on the liberation of (125)I T-4 from a tagged euthyroid serum reagent, the thyroxine being liberated by alcohol denaturation from 0.5 ml. of patient serum.We have established that there is proceeding simultaneously a second mechanism of T-4 liberation which is in no way associated with patient thyroxine. A simple technique for determining the extent of, and correcting for this second reaction is described.The procedure employs the same equipment used for the (125)I T-3 and involves only one additional step. No calibration curve is required. Results are not influenced by iodine in any form.The T-4 content is best expressed as a ratio to that of a standard mid-euthyroid serum. The ratio values for the hypothyroid, hyperthyroid and euthyroid states are characteristic and free from overlap. Separation between low normals and hypothyroids is very sharp. The relation between the serum T-4 ratio and the assayed thyroxine content has a correlation coefficient of 0.91. The product of the (125)I T-3 ratio and the T-4 ratio makes the procedure applicable in pregnancy and during steroid use, and provides a good indication of the free thyroxine present.The values of T-4, the (125)I T-3 and the PBI have been applied to 1084 patient sera and the T-4 and the (125)I T-3 to an additional 616. In this series of 1700 patients the serum T-4 was found to indicate the most probable clinical classification with a reliability of at least 95%.

Age Factors↗