PubMed Health⌕ Search

Biomedical subjects

J J Miescier

Publications and source records attributed to J J Miescier.

4 recordsLinked to original sources
PubMed1980

Commentary on AOAC method for paralytic shellfish poisoning.

Paralytic shellfish poisoning (PSP) is caused by ingesting bivalve molluscan shellfish which have fed on the toxigenic marine dinoflagellates Gonyaulax catanella or G. tamarensis. The toxins from these organisms are neurotoxic alkaloids which interfere with nerve conduction and block muscle-action potential. The U.S. Food and Drug Administration cooperates with state shellfish control officials in the National Shellfish Sanitation Program to prevent marketing of toxin-contaminated shellfish. The toxins are quantitated by the standard mouse bioassay method, as found in Official Methods of Analysis of the AOAC. This paper discusses the procedure followed in the standard bioassay method in an attempt to clarify for the PSP analyst the rather complex official methodology, and, thus, promote more uniform results among laboratories.

Animals↗
PubMed1978

Fecal coliform methods for examination of sea water: interlaboratory evaluation of split sample analysis.

An interlaboratory study was conducted to compare the effectiveness of the following 3 multiple-tube fermentation methods for determining the most probable number (MPN) of Escherichia coli in a split artificial sea water sample: (1) the 72-hr standard methods procedure of the American Public Health Association, (2) a 24-hr elevated-temperature test using A-1 medium, and (3) a 24-hr elevated temperature test modified to include an initial 3-hr resuscitation period using A-1 medium. The capability of the laboratories to perform the 3 test procedures was also compared. Split sample replicates with low, medium, and high levels of E. coli were examined in 18 laboratories in the United States and Canada. Data indicate that the laboratories performed each test with equal capability, and all 3 procedures were equally effective in enumerating the strain of E. coli used in this investigation. By virtue of its homogeneity and stability, the split sample served as an appropriate specimen for this study and could probably be used as a proficiency test specimen for evaluating laboratory analyst performance in the bacteriological examination of sea water.

Bacteriological Techniques↗