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J J Trout

Publications and source records attributed to J J Trout.

28 records · Page 2Linked to original sources
PubMed1981

Increased autophagy in chloroquine-treated tonic and phasic muscles: an alternative view.

Ultrastructural and cytochemical techniques were used to investigate autophagy in the tonic anterior(ALD) and phasic posterior (PLD) latissimus dorsi muscles of the chicken following chloroquine administration. Autophagic vacuoles were seen in the ALD after 1 day of chloroquine administration while no change was seen in the PLD until 3 days. In both muscles, autophagic vacuoles and myeloid bodies were found at the level of the I band. Myeloid bodies usually were found in the longitudinal rows of mitochondria in the ALD muscle. Some, but not all, of the autophagic vacuoles and myeloid bodies were cytochemically acid phosphatase positive, while the portion of the sarcoplasmic reticulum of both muscles which is normally acid phosphatase positive, while the portion of the sarcoplasmic reticulum of both muscles which is normally, acid phosphatase positive was devoid of activity following chloroquine administration. These observations are discussed in regard to accepted mechanisms of autophagy and the possible inhibition of autophagy in skeletal muscle tissue by chloroquine.

Acid Phosphatase↗
PubMed1979

A unique acid phosphatase location: the transverse tubule of avian fast muscle.

Acid phosphatase activity was localized cytochemically in the posterior latissimus dorsi muscle of the chicken. Reaction product was observed in three distinct structures: T-tubules, sarcoplasmic reticulum and dense bodies. Examination of cross- and longitudinal sections confirmed that the reaction product was membrane-limited. Acid phosphatase activity was observed in sarcoplasmic reticulum adjacent to the A-I junction and the A-band, in termyofibrillar dense bodies located along the length of the fibre and in the T-tubules but not in the surface caveolae or in the lateral sacs of the sarcoplasmic reticulum. The uniqueness of the T-tubular localization with respect to cytochemical localizations in other muscles is discussed.

Acid Phosphatase↗
PubMed1979

Cytochemical observations of two distinct acid phosphatase-reactive structures in anterior latissimus dorsi muscle of the chicken.

Acid phosphatase activity has been localized cytochemically in the avian anterior latissimus dorsi muscle in two distinct structures, the sarcoplasmic reticulum and membrane-limited dense bodies. Cross and longitudinal sections confirmed that the reaction product was membrane-bound. Acid phosphatase activity was observed in the longitudinal tubules of the sarcoplasmic reticulum in the region of the I band and in dense bodies located along the length of the fibre just beneath the sarcolemma and between the myofibrils. This dual localization is discussed in relationship to previous cytochemical and zonal centrifugation evidence for more than one acid phosphatase-containing organelle in skeletal muscle.

Acid Phosphatase↗
PubMed1978

Some cellular changes associated with triton WR-1339 accumulation in rat hepatocytes.

Electron microscopic morphological and cytochemical techniques were used to follow the sub-cellular events that accompanied Triton WR-1339 accumulation in hepatocytes. Localization of two lysosomal enzymes, acid phosphatase and aryl sulfatase, clearly established the lysosomal nature of the Triton WR-1339 containing electron-lucid structures that appear in hepatocytes following treatment with this compound.

Acid Phosphatase↗