[Somatic effects of radiation].
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Biomedical subjects
Publications and source records attributed to J Jaśkowski.
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Rat skin was deprived of epidermis and the wound was exposed to ions generated with Bion 80 apparatus, for 3 h, just after wounding, only once. In comparison with the control, positive air ions retarded and negative air ions accelerated the wound healing. This effect was almost the same when air, nitrogen, oxygen and carbon dioxide were applied. A direct action of air ions is suggested.
The effect of exposure of L 1210 mouse leukemia cells to artificially generated air ions on the activity of membrane Na, K-ATPase in the cells was investigated. The exposure of cells to air ions of both signs gave identical results, i.e. diminution of transport activity of the enzyme, measured by radioactive 86Rb transport into the cell (ouabain-dependent). Passive ouabain-independent transport of Rb+ into the cells remained unchanged in the air ion-treated cells, as did the passive efflux of the radioisotope from preloaded cells. The possible explanation of the phenomena observed is discussed.
The ability of air ions to produce changes in the electrical properties of L 1210 mouse leukemia cells was tested. The fluorescence of ANS incorporated into a membrane lipid bilayer (measured microphotometrically) was used as a probe. It was shown that the action of air ions of both signs could change (negative ions by increasing and the positive ones by decreasing) the fluorescence intensity of ANS in the cell surface structures or to an imbalance of ions inside and outside the cell. Both possibilities are discussed in the light of the results of experiments using ouabain or biguanide as factors diminishing the intensity of ANS fluorescence.
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