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J Jelínková

Publications and source records attributed to J Jelínková.

At least 19 recordsLinked to original sources
PubMed2004

[Aspects of positive feelings of pregnancy].

OBJECTIVE: To analyze the relation of positive feeling in pregnancy with intentional conception, to analyze the relation between feeling the pregnancy by the women and her partner. TYPE OF STUDY: A questionnaire investigation in a representative cohort. SETTING: Ward for Preventive and Social Pediatrics, Medical Faculty, Masaryk University, Brno. METHODS: Frequency analysis, Chi-square, F-test. RESULTS: Positive feeling in pregnancy should not be simply associated with intentional pregnancy. In spite of the fact that planned pregnancies are mostly accepted positively, it does not necessarily mean that opposite is true in cases of unplanned gravidity. In the course of pregnancy the positive feeling is growing and the number of unhappy women decreases to a minimum. In women who positively accepted their pregnancy, we found out frequent support by their partner. It is of interest that the attitude of the partner in some cases proved to be more favorable than that of the woman herself. CONCLUSION: Results of this investigation revealed usefulness of differentiating between intentional conception and its emotional experience, since unplanned pregnancy does not necessarily follow to negative feeling from the position of the woman. On the contrary, partners of the pregnant women accepted pregnancy favorably in most cases.

Attitude↗
PubMed2001

Mortality during winter smog episodes 1982, 1985, 1987 and 1993 in the Czech Republic.

OBJECTIVES: Severe air pollution episodes were recorded during the 1980s and early 1990s in the Czech Republic as a result of widespread combustion of brown coal. A population-based retrospective study investigated the relationship between air pollution and daily mortality in six highly polluted areas of the Czech Republic during smog episodes in 1982, 1985, 1987, and 1993. METHODS: Total daily mortality, mortality by gender and age, cardiovascular mortality, respiratory mortality, data on weekly incidence of acute respiratory diseases and daily mean concentrations of sulphur dioxide and suspended particulate matter were used in the model. The effects of smog on daily mortality were estimated by multiple linear regression analysis. RESULTS: Significant increases in mortality were observed for the 1982 and 1987 episodes (6% and 9%). In 1982, mortality was significantly associated with mean concentration of sulphur dioxide (SO2) of the current and the preceding days and with the 4-day moving average. In the 1985 episode a significant increase in respiratory mortality in men and in both genders together, lagging by 2 and 3 days, was detected. During the 1987 episode significant associations of total daily mortality, mortality in persons over 65 years of age and mortality from cardiovascular or respiratory diseases with 4-day moving average of both pollutants were found. For the 1993 episode a significant association between mortality in women under 65, lagging by 3 days, and mean concentration of suspended particulate matter (SPM) was observed. CONCLUSIONS: Most of the results are consistent with other studies aimed at episodic air pollution during the 1950s and 1960s in Western Europe and the USA, in which outdoor air pollution was shown to be a significant predictor of mortality. However, non-significant or opposite associations between air pollution and mortality indicate that other factors may also play an important role. A stronger effect on men under 65 years of age, suggested by a previous Czech study was not confirmed.

Czech Republic↗
PubMed1993

Immunochemistry of capsular type polysaccharide and virulence properties of type VI Streptococcus agalactiae (group B streptococci).

The immunochemistry of capsular type polysaccharide and virulence characteristics of group B streptococci (GBS), type VI, were studied. By high-pressure anion-exchange chromatography and pulsed amperometric detection, as well as by 13C nuclear magnetic resonance analysis, both extracellular and cell-bound polysaccharides were found to contain glucose, galactose, and N-acetylneuraminic acid in the molar ratio of 2:2:1, respectively. At variance with all other GBS serotypes described to date (Ia, Ib, II, III, IV, and V), no N-acetylglucosamine was present, whatever the source of the material (secreted or cell bound; reference or clinical isolate). Sialic acid was probably involved in the immunodeterminant structure of this new serotype since cleavage of this sugar from the polysaccharide gave rise to an antigen which reacted very weakly with type VI antiserum and to a precipitation line in immunodiffusion with no identity with the native type VI polysaccharide. By using type VI antiserum and the protein A-gold technique, a large capsule was observed in the type VI GBS reference strain by electron microscopy. All type VI strains examined were lethal for CD-1 mice, the 50% lethal dose after intraperitoneal challenge ranging from 1.0 (+/- 0.9, standard deviation) x 10(5) to 2.5 (+/- 1.5, standard deviation) x 10(5) CFU per mouse. A rabbit antiserum against capsular type polysaccharide exhibited both protective activity for mice injected intraperitoneally with type VI reference strain or with clinical isolates and opsonic activity in a phagocytosis assay.

Acetylglucosamine↗
PubMed1993

[Differentiation of serologic group A streptococci from group C and G using disks with various bacitracin levels].

It was revealed that a diameter of the inhibition zone of 10 mm or more surrounding a disk with 0.04 U bacitracin differentiates reliably strains of the serological group A form strains of group C and G with the exception of 7.3% strains of group C and G with a high susceptibility for bacitracin. The disks with a higher bacitracin content gives less reliable results.

Bacitracin↗
PubMed1991

Structural determination and immunochemical characterization of the type V group B Streptococcus capsular polysaccharide.

The type V capsular polysaccharide of group B Streptococcus has been isolated and purified, and its repeating unit structure determined. The native type V polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in a molar ratio of 3:2:1:1. Methylation analysis and 1H NMR and 13C NMR analysis of the native type V polysaccharide and of its specifically degraded products permitted the determination of the repeating unit structure of the type V polysaccharide: [formula: see text] The type V polysaccharide has certain structural features in common with other group B streptococcal capsular polysaccharides but is antigenically distinct: no immunologic cross-reactivity was observed between type V and types Ia, Ib, II, III, or IV polysaccharides. Studies of antibody binding to the partially degraded forms of the type V polysaccharide indicated that the native epitope is complex, involving most if not all of the sugar residues of the repeating unit.

Bacterial Capsules↗
PubMed1989

Postnatal development of plasma FFA concentration and its influence of the separation of bilirubin from albumin binding in newborns.

The first part of the study gives data on the FFA concentrations, glycaemia and energy quotient in newborns with uncomplicated postnatal adaptation in the first 2 weeks of life. The study was carried out in a group of 69 full-term and 69 pre-term infants at the age of 24, 48, 72, 96 hours and 7 and 14 days. Although it is not the case of consecutive values of FFA concentrations in plasma, a certain developmental trend can be suggested. This is analogous in both groups with the only difference that in pre-term infants FFA culminate and decrease earlier (pre-term 48-72 hours, full-term infants 72-96 hours). The study shows that, in pre-term and full-term infants with satisfactory postnatal adaptation and above nutrition, stabilization of glucose-lipid metabolism occurs on the 3rd and 4th day after birth, respectively. In pre-term infants supplementary nutrition by the glucose system (glucose and amino acids) had a favourable effect. The second part of the study assesses the potential risk of increasing plasma FFA concentration for the separation of bilirubin from albumin binding. The means plasma FFA concentrations reached 25-48 and 49-72 hours after birth had no significant effect on the BCA/Bi ratio and, therefore, they are not dangerous as regards the possibility of displacing bilirubin from albumin binding.

Bilirubin↗
PubMed1988

Immuno-electronmicroscopic demonstration of capsules on group-B streptococci of new serotypes and type candidates.

The location of type polysaccharides on the cells of reference strains of group-B streptococci of serotypes IV and V and new type candidates NT6 and 7271 was investigated by electronmicroscopy of the bacteria after incubation with homologous type-specific antiserum. A distinct capsular layer was found on the surface of the cells of all these strains. Sialic acid, an integral part of all the conventional type polysaccharides of group-B streptococci, was also detected in all the strains examined.

Immunologic Techniques↗
PubMed1986

The adhesin structures involved in the adherence of group B streptococci to human vaginal cells.

The adherence of group B streptococci (GBS) of serotypes Ia, II and III to human vaginal cells was studied in vitro. The adherence was not dependent on the viability of bacteria; killing of GBS by UV irradiation or glutaraldehyde treatment did not inhibit the adherence. Killing of GBS by heating to 56 degrees C for 1 h led to a pronounced decrease of adherence, demonstrating the thermosensitivity of the GBS structures involved. The protein nature of these structures was proved by a significant reduction of adherence after pretreatment of GBS with trypsin or pepsin. Pretreatment of GBS with sialidase had no influence on the adherence. Such a pretreatment of vaginal cells caused an increase of adherence showing that the receptors on epithelial cells may be partly masked by sialic acid.

Bacterial Adhesion↗
PubMed1986

Adherence of vaginal and pharyngeal strains of group B streptococci to human vaginal and pharyngeal epithelial cells.

In vitro tests for adherence to human vaginal and pharyngeal epithelial cells were used to study the problem of tissue-specific tropism in group B streptococci (GBS). Twenty-two vaginal or pharyngeal clinical isolates of GBS (serotypes Ia, Ib, II, and III) were used. No significant differences in adherence to vaginal and pharyngeal epithelial cells were found between GBS from both sources: statistical analysis furnished no evidence for tissue-specific tropism. Serotype III vaginal GBS adhered better to vaginal and pharyngeal epithelial cells than did serotype III GBS strains isolated from the pharynx. However, pronounced differences in the level of adherence were found among strains of the same serotypes and from the same sources. Thus, the results obtained suggest that differences in adherence may rather be strain-dependent that type-dependent.

Bacterial Adhesion↗
PubMed1986

A search for new group-B streptococcal serotypes.

New serotypes were sought among 165 clinical isolates of group-B streptococci that were untypable by antisera for the conventional types Ia, Ib, II and III. The strains were tested for sialic acid, an integral component of the group-B streptococcal type-polysaccharides; trypsin-treated bacteria were tested by slide-agglutination with the sialic-acid-specific lectin from the snail Cepaea hortensis. Sialic acid was detected in 96 of the strains; in 95 of these, new type antigens were identified serologically (type IV, 52; provisional type V, 34; candidate type NT6, seven; provisional type V and candidate type NT6, one; candidate type 7271, one); the remaining strain was found to possess a small amount of Ia antigen. Sialic acid was not detected in 69 strains, and none of these possessed a polysaccharide type-antigen. Chemical measurement of the sialic-acid content of cultures by Aminoff's method gave results in conformity with the lectin-agglutination test and the presence of polysaccharide type-antigens.

Agglutination Tests↗
PubMed1985

Worldwide distribution of two new serotypes of group B streptococci: type IV and provisional type V.

The candidates for new types proposed by the laboratories of H. W. Wilkinson, J. Jelínková, B. Perch, and S. Rabinowitz were antigenically compared and found to be distinct from the established types. On the other hand, identity between some of the candidates was found: three belonged to the new type IV and two shared the NT1 antigen. Strains with NT1 antigen were given the working label "provisional type V." A distribution study identified 102 type IV strains and 53 provisional type V strains among isolates from 15 countries.

Antigens, Bacterial↗
PubMed1984

[Submicroscopic aspects of the adherence of group B streptococci to vaginal epithelial cells].

The submicroscopic structure of surfaces in the streptococci of group B, type III (strain 13/63) and the ultrastructure of the interaction of this streptococcus strain with human vaginal cells were studied. The surface of the majority of B streptococci was smooth after using the conventional fixing techniques of electron microscopy; however, about 25% of streptococcal cells had an additional layer of filamentous protrusions on their surface. A marked layer of capsular material was visualized by means of the preincubation of this streptococcus strain with a type-specific antiserum. The incubation of B streptococci with vaginal cells without any addition of antibody allowed for the demonstration of the contact of a part of the cells of the bacterial population with the epithelial cells through filamentous protrusions. However, the majority of the smooth-surface cells was separated from vaginal cells by a gap wide up to 150 nm. It was demonstrated by additional incubation with type-specific antiserum that the above mentioned gap corresponded to the capsular substance of bacteria. Hence the capsule of streptococci in group B is the basic component of their surface responsible for adherence to vaginal cells. Adherence of B streptococci to vaginal epithelia was accompanied neither by bacterium ingestion nor by the destruction of epithelial cells.

Adhesiveness↗
PubMed1979

Prospective study on streptococcal pharyngitis among a town population.

A one-year-round study on pharyngitis incidence among the general population of a town (24,300 inhabitants) was carried out. All patients, with pharyngitis who visited health centres were examined clinically and mcirobiologically. The overall pharyngitis incidence rate and the streptococcal pharyngitis incidence rate were, respectively, 8.3 and 3.9 cases per 100 population. Age-related incidence was highest in the group of 5-10 years, seasonal incidence was highest in autumn. Clinical diagnoses made preliminary to bacteriological examination were correct in only a half of the streptococcal pharyngitis cases. The accuracy of clinical diagnosis of streptococcal pharyngitis could, at best, be raised to 80% if fever of less than or equal to 38.0 degrees C and presence of exudate on tonsils should be considered significant criteria. A great majority of the streptococcal pharyngitis cases were caused by group A streptococci; the prevailing M types were 12, 1 and 3. Emphasis is laid on the necessity of performing microbiological examination of all pharyngitis cases in order to ensure etiological diagnosis and causal therapy. 15)

Adolescent↗
PubMed1978

Group B streptococci in the female genital tract and nosocomial colonization of newborns.

In a long-term study, rates for group B streptococcus prevalence in the vagina and/or anus of nonpregnant women and pregnant women in the first trimester and in the second trimester were found as follows: 7.1%, 7.3% and 5.4% respectively. At delivery, positivity rates of 4.8% and 3.8% were found for mothers and newborns, respectively. Each pair of strains isolated from mother and newborn was of the same serotype. In 13.6% of newborns nosocomial colonization of the upper respiratory tract occurred during their stay in the maternity home. The sources were other positive newborns sharing the same boxes, the nosocomial spread proceeded in clusters. Detailed type-antigen analysis of isolates allowed identification of index cases and their direct contacts. Types Ib, Ic, II/R, III + Ic predominated. Sexual transmission was demonstrated by findings of identical serotypes in both partners: 23% of male partners of nonpregnant positive women and 9.1% of partners of pregnant positive women had positive urine samples. A control male group consisting of psychiatric sanatorium patients without sexual contact repeatedly exhibited a positivity rate of 2%.

Anal Canal↗