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Biomedical subjects

J Karkos

Publications and source records attributed to J Karkos.

26 records · Page 2Linked to original sources
PubMedDate not supplied

[Morphological studies of the brain in rats receiving meta-bromophenylsuccinimide].

Morphological evaluation of changes in the brain of rats receiving orally metabromophenylsuccinimid (4-m) - original Polish antiepileptic drug - 1/20 of DL50 per day during a period of one month, has been performed. The dose of the preparation per one kg of the body weight was approximately nine times higher than the therapeutic dose applied at present in human. Neuropathologic changes were found only occasionally; they were not specific, degenerative in character. Both parenchymal and mesenchymal components of the nervous tissue were involved. In neurones the lesions characteristic of chronic disease could be seen; they were more conspiecuous in the cortex of the cerebral hemispheres and in the cerebellum. The damage of the neurones was accompanied by a marked glial cells reaction, progressive in character. Morphological changes of the vessels with consecutive oedema of the white matter were limited to endothelium. Sporadic and not extensive character of the changes as well as their mild intensity seem to provide an evidence that the neurotoxic effect of the tested preparation m-4 is relatively small.

Administration, Oral↗
PubMedDate not supplied

Evaluation of the method of testing on spermatozoa of the local irritant effect of streptomycin.

This report presents an attempt to adapt the method of study of the cytotoxic effect of streptomycin (SM) for evaluation of its local irritant properties. The cytotoxic effect of streptomycin was treated on bull spermatoza according to Dumitrescu (1). Ten series of SM produced by Tarchomin Pharmaceutical Producers, and international standard streptomycin (Is -- SM) were used in the experiments. The effect of the mentioned substances was checked in isotonic solutions (in 5% glucose containing 0.82, 2.46, 7.38 mg SM base in 1 ml at pH 4.5--4.8). Is -- SM did not show any spermicidal effect, on the contrary, it significantly prolonged the life-span of spermatozoa. The checked series showed statistically significant spermicidal activity. The cytotoxic effect of SM is proportional to its concentration, and varies in particular series. The studied series can be divided into those of strong, moderate, and weak spermicidal activity. The comparison of these results with those of simultaneous clinical and histopathological studies indicates no correlation between the local irritating effect observed in humans, and the cytotoxic influence determined in the test with spermatozoa. The method of study of SM on spermatozoa is unsuitable for evaluation of the local irritating activity of this substance.

Animals↗