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Biomedical subjects

J Kedziora

Publications and source records attributed to J Kedziora.

At least 37 records · Page 2Linked to original sources
PubMed1996

[Metabolic changes in blood oxygen in the human heart under ischemic and reperfusion conditions].

The number of granulocytes, their capability to generate O2-. and the activity of SOD-1, GSH-Px, Cat as well as MDA concentrations in erythrocytes in the blood extracted from the venous sinus and aorta under coronary artery bypass with use of St. Thomas cardioplegic solution were determined. The blood for examination was obtained before the institution of cardiopulmonary bypass, in the period of the deepest ischaemia (just after declamping of the aorta) and between the 1-3 minute and the 10-13 minute of reperfusion. A rise in the number of granulocytes both in the venous sinus and aortal blood at all examined intervals was noted. Capability to produce superoxide anion radicals decreased at the peak of ischemia and during reperfusion. The activity of SOD-1 was lower both after the period of ischemia and reperfusion. A rise in aortal blood activity during reperfusion was characteristic for GSH-Px; the activity was greater in the blood sampled from the coronary sinus during ischemia and initial reperfusion. With the exception of the initial reperfusion the activity of Cat diminished in all observed cases. We did not observe any significant changes in MDA concentration with the exception of the initial reperfusion in the aortal blood and later during reperfusion in the blood from the coronary sinus. The results demonstrate that the applied cardioplegic solution may protect myocardium from harmful effects of active oxygen froms produced as a results of ischemia and reperfusion.

Adult↗
PubMed1995

Estimation of cholesterol sulphate in blood plasma and in erythrocyte membranes from individuals with Down's syndrome or diabetes mellitus type I.

OBJECTIVES: Plasma and erythrocyte membrane cholesterol sulphate (CS) were measured in patients suffering from diabetes and Down's syndrome. DESIGN AND METHODS: The procedure for separation and determination of CS comprised HPTLC (high-performance thin-layer chromatography) and densitometry. RESULTS: The mean plasma and RBC membranes CS concentrations (+/- SD) of the control group (n = 16) was 188 +/- 47 micrograms/dL and 343 +/- 57 micrograms/10(12) RBC, respectively. In 15 patients with diabetes and 12 Down's syndrome patients substantially higher CS levels were found (diabetes: plasma-348 +/- 60 micrograms/dL; RBC membranes-646 +/- 113 micrograms/10(12) RBC; Down's syndrome: plasma-245 +/- 54 micrograms/dL; RBC membranes 427 +/- 74 micrograms/10(12) RBC). Analysis of variance and multiple comparison (Newman-Keuls test) show statistically significant differences between all samples both for erythrocytes, F(2.41) = 52.24, p < 0.05, and plasma, F(2.41) = 34.92, p < 0.05. CONCLUSIONS: It is postulated that differences in CS levels may contribute to changes of erythrocyte properties in these pathological states.

Adult↗
PubMed1995

Activities of acetylcholinesterase and NA+, K+ -ATP-ase in human erythrocytes after ethanol consumption.

The influence of ethanol consumption on acetylcholinesterase (AchE) and Na+, K+ -ATP-ase activities was examined in 14 healthy volunteers after 30 minutes of ethanol treatment at a dose 0.6 g/kg of body weight. It was observed that the activity of both enzymes decreased. The activity of AchE was significantly lower, and that of Na+, K+ -ATP-ase tended to be lower, but the difference was not significant. In vitro experiments showed that ethanol inhibited the AchE and N+, K+ -ATP-ase activities immediately and in proportion to the concentration of ethanol used.

Acetylcholinesterase↗
PubMed1995

Oxygen metabolism in blood of patients with gastric and duodenal ulcer disease.

Among multiagent mechanisms causing peptic ulcer disease, oxygen free radicals hypothesis is reasonable. Our investigation concerned the determination of oxygen free radicals generation lipid peroxidation and enzymatic antioxidative defense in blood of patients with peptic ulcer disease. The examinations were performed in 79 males, non-smokers, aged 20-51 with peptic ulcer disease, clinically diagnosed as gastric or duodenal ulcer. Biochemical investigations were carried out on blood platelets obtained from venous blood according to the centrifugation fractionation technique. Superoxide dismutase (CuZn-SOD) (EC 1.15.1.1.) activity and malonyl dialdehyde (MDA) concentration were determined according to Misra and Fridovich (1972) and Placer et al (1966) methods, respectively. Oxygen free radicals as the superoxide anion O2- generation in blood according to Bellavite et al. (1983) method was determined. The results indicate that patients with peptic ulcer disease have a higher level of malonyl dialdehyde in blood platelets, whereas the superoxide dismutase activity is significantly decreased in comparison with the control group. Oxygen free radicals generation both in patients with peptic ulcer disease as in healthy men is maintained at normal level. Our data suggest that in spite of similar range of oxygen free radicals generation both in patients with peptic ulcer disease and healthy men, the injury to patients by superoxide anion is more destructive and harmful to cells, because the enzymatic antioxidative defense is decreased. The increase of lipid peroxidation in patients with peptic ulcer disease also supports this conclusion.

Adult↗
PubMed1995

Effects of submaximal physical exercise and immobilization in bed on the adenine nucleotides concentration in human blood platelets.

The effects of submaximal physical exercise (75% VO2max) and one month immobilization in bed on the adenine nucleotides concentration in human blood platelets were studied and compared. We have shown that after submaximal exercise and after immobilization in bed the concentrations of adenine nucleotides in blood platelets mainly ATP and ADP were decreased and probably caused by the stimulation of platelet secretory process. Thrombin which stimulated the release of 58.2% +/- 1.5% of total adenine nucleotides from control platelets, liberated decreased amounts of nucleotides from platelets after physical exercise and after immobilization in bed (39.9 +/- 2.1% and 23.6 +/- 2.03% of total, respectively).

Adenine Nucleotides↗
PubMed1995

Effect of ethanol on human erythrocyte superoxide dismutase activity and malonyl dialdehyde concentration.

The effect of a single administration of ethanol on the erythrocyte superoxide dismutase activity and malonyl dialdehyde concentration was examined in 14 healthy volunteers. We observed that 30 min after ethanol consumption in a dose 0.6 g/kg of body weight, superoxide dismutase activity was significantly higher as compared to that before ethanol treatment. No changes in susceptibility of erythrocytes to lipid peroxidation was observed after ethanol consumption. There was no significant changes in the malonyl dialdehyde concentration before and after ethanol treatment.

Adult↗
PubMed1995

[Generation of active oxygen species by neutrophils in patients with unstable angina pectoris].

The generation of O2-. and H2O2 by neutrophils (PMNs) obtained from peripheral and coronary sinus blood was investigated in twenty four patients with unstable angina, who were qualified for PTCA procedure. The control group consisted of twenty one clinically healthy persons. The blood for the investigations was obtained from coronary sinus and basilic vein just before the procedure, while in the control group from--basilic vein only. In patients with unstable angina statistically significant higher (p < 0.05) O2-. generation by peripheral blood PMNs (at rest and stimulated), compared with the control group, was found respectively (the sick: rest--11,09 +/- 1.92; stimulated--25.48 +/- 5.76 nmol/cell/min.; healthy: rest--7.98 +/- 1.06; stimulated--13.58 +/- 1.19 nmol/cell/min). No significant differences in O2-. generation between PMNs obtained from coronary sinus or peripheral blood were found. No differences in H2O2 generation by rest PMNs obtained from coronary sinus or peripheral blood were found in patients compared with the control group. The generation of H2O2 by PMA stimulated PMNs was higher in the sick without statistical significance.

Adult↗
PubMed1995

Red blood cell metabolism in men during long term bed rest.

The investigations were carried out on 20 men (aged 32-48), hospitalized after lower extremity fractures, in whom no systemic diseases or disorders were found. The activity of glucose-6-phosphate dehydrogenase (G6P-DH), aldolase (A1), lactate dehydrogenase (LDH) and the concentrations of pyruvate, lactate, nicotinamide-adenine dinucleotide (NAD), nicotinamideadenine dinucleotide phosphate (NADP), adenosine-5'-triphosphate (ATP), adenosine-5'-diphosphate (ADP), adenosine -5'-monophosphate (AMP) and 2,3-diphosphoglycerate (2,3-DPG) in erythrocytes taken from patients were estimated. After a two-week hypokinesia G6P-DH, A1, LDH activities and ATP, ADP, AMP concentrations decreased, whereas other estimated parameters did not differ from the control levels. During a 30-day hypokinesia of patients with fractures of the lower extremity a transient decrease of erythrocyte metabolic activity was observed after two weeks. off

Adult↗
PubMed1995

[Oxygen-derived free radicals of granulocytes in the blood of patients with chronic inflammation of the middle ear and palatine tonsils].

The evaluation of oxygen species generation in blood granulocytes of patients with chronic inflammation of middle ear and palatine tonils. There were 38 patients tested (10 with chronic inflammation of palatine tonsil, 18 with chronic inflammation of middle ear and 10 healthy ones). There were statistically significant differences between the superoxide anion generation in patients with chronic inflammation of palatine tonsil and middle ear in comparison with to the healthy ones.

Adult↗
PubMed1995

[The generation of oxygen free radicals in blood in patients with hypertrophy of paranasal sinuses and larynx].

The evaluated of oxygen specious generation in blood granulocytes of patients with hypertrophy of paranasal sinuses and larynx. There were 33 patients tested (13 with hypertrophy of paranasal sinuses, 10 with hypertrophy of larynx and 10 healthy). The superoxide anion generation of peripheral blood granulocytes described by the rate of the reduced cytochrome C at rest and after stimulation with opsonized zymosan according to Bellavite were measured. There were statistically significant differences between the superoxide anion generation in patients with hypertrophy of paranasal sinuses and larynx in comparison with the healthy ones.

Adult↗
PubMed1994

[Some indices of peripheral blood oxidative metabolism during acetate and bicarbonate hemodialysis in patients with chronic uremia].

Whole blood superoxide anion generation, erythrocyte superoxide dismutase (SOD-1) and catalase activities and erythrocyte and plasma malonyldialdehyde concentrations were evaluated during cuprophane hemodialysis using bicarbonate and acetate dialysate. Superoxide anion generation by resting phagocytes was found to be significantly higher during bicarbonate hemodialysis, while its generation by opsonized zymosan--stimulated phagocytes did not differ between hemodialyses with both kinds of dialysates. Changes in the erythrocytes SOD-1 activities, found in the initial period of hemodialysis, were independent of the kind of dialysate, while erythrocyte catalase activity was higher during acetate hemodialysis in comparison with bicarbonate one. The kind of dialysate did not affect extent of erythrocyte membrane lipid peroxidation, while plasma lipid peroxidation was lower during acetate hemodialysis in comparison with bicarbonate one. In vitro studies with blood cells incubated with acetate or bicarbonate ions in concentrations, which are observed in vivo during hemodialysis, suggest that probably these ions do not directly affect superoxide anions generation, erythrocyte SOD-1 and catalase activities and erythrocyte membrane lipid peroxidation.

Acetates↗
PubMed1994

[Influence of percutaneous transluminal coronary angioplasty (PTCA) on superoxide anion generation by whole blood granulocytes in patients with unstable angina pectoris (part I)].

In twenty patients with unstable angina, the effects of percutaneous transluminal coronary angioplasty (PTCA) on superoxide anion generation by neutrophils (PMNs) obtained from ulnar vein (UV) and coronary sinus (CS) were investigated. Blood samples for the investigations were taken from CS just before the procedure, within the first minute after the last balloon inflation and 20 minutes after PTCA; from BV--just before the procedure and 20 minutes following it. The results were compared with those obtained from 12 patients with unstable angina, (in whom coronary angiography was performed, but for various reasons angioplasty was desisted from) and with those obtained from 20 clinically healthy persons. In patients with unstable angina, the significantly increased O2.- generation by PMNs from CS and BV was found at rest as well as following opsonized-zymosan stimulation, compared with healthy persons. Within the first minute after PTCA, the significantly decreased O2.- generation by PMNs from CS was observed, compared with the results before the procedure (rest.--11.29 +/- 0.73 and 10.15 +/- 0.65 nmol/cell/min., p < 0.05; stimulation--27.62 +/- 6.25 and 18.54 +/- 3.37 nmol/cell/min., p < 0.05). Twenty minutes after PTCA O2.- generation by PMNs from CS still decreased (rest--9.23 +/- 1.08 nmol/cell/min., stimulation--15.71 +/- 5.98 nmol/cell/min.). In blood samples taken from BV before and after PTCA, no statistically significant differences in O2.- generation were observed (rest--11.32 +/- 0.79 nmol/cell/min. and 10.77 +/- 1.43 nmol/cell/min.; stimulation--25.96 +/- 6.12 nmol/cell/min. and 21.15 +/- 5.47 nmol/cell/min.).

Adult↗
PubMed1994

[Effect of gamma radiation on levels of adenine nucleotides in erythrocytes of healthy individuals after submaximum physical exertion].

The authors studied the effect of gamma radiation and submaximum physical exercise on adenosine-5'-triphosphate (ATP), adenosine-5'-diphosphate (ADP) and adenosine-5'-monophosphate (AMP) contents in erythrocytes of healthy males. Twenty one men aged 20-22 years were examined. They underwent physical exercise at doses of 2 w/kg body weight for 15 min. Erythrocytes were exposed to gamma radiation (500 gy doses) from 60Co source. The concentration of adenine nucleotides in erythrocytes was measured by the Boehringer Mannheim tests. The submaximum physical exercise was found to decrease ATP content and to to increase ADP and AMP in erythrocytes. Gamma radiation at 500 Gy dose was found to decrease ATP concentration in erythrocytes both at rest and after submaximum exercise and to increase AD content. It was revealed that AMP content increased at rest and decreased after submaximum exercise in irradiated erythrocytes.

Adenosine Diphosphate↗
PubMed1993

Changes in antioxidant enzymes activities, aggregability and malonyldialdehyde concentration in blood platelets from patients with coronary heart disease.

In 41 patients with coronary heart disease (CHD) the concentrations of total blood platelet malonyldialdehyde (MDA: 2.11 +/- 0.25 nmol/10(9) platelets) and MDA corresponding to thromboxane A2 (TXA2 0.84 +/- 0.13 nmol/10(9) platelets) were increased in comparison with values in blood platelets of healthy subjects (1.19 +/- 0.09 and 0.71 +/- 0.05 nmol/10(9) platelets), respectively. The increased aggregability with ADP and thrombin of patient platelets was also observed. In relation to the blood platelets of healthy subjects, the antioxidant enzymes activities of patient blood platelets were significantly (P < 0.001) decreased. Platelet glutathione peroxidase (GSH-Px) activity of the patients (11.3 +/- 0.85 U/g protein) was significantly lower than controls (18.3 +/- 1.12 U/g protein). In patients with CHD the activities of the other antioxidative platelet enzymes: catalase (Cat, 7.37 +/- 1.38 U/g protein) and superoxide dismutase (SOD, 1529.4 +/- 167 U/g protein) were also significantly decreased in comparison with values for healthy subjects (Cat: 9.06 +/- 1.30 U/g protein and SOD: 1987 +/- 230 U/g protein, respectively). It is suggested that antioxidative defense in blood platelets may affect the haemostatic processes and lipid peroxidation in patients with CHD.

Adenosine Diphosphate↗
PubMed1993

[Effect of gamma radiation on the concentration of pyruvate and lactate in erythrocytes of healthy men after submaximal physical exercise].

The aim of this work was to study the effect of gamma radiation and submaximal physical exercise on the concentration of final products of anaerobic glycolytic pathway in erythrocytes of healthy men. Twenty one men aged 20-22 were examined. They underwent physical exercise at doses of 2 w/kg body weight for 15 min. Erythrocytes were taken in the rest and after physical exercise and were exposed to gamma radiation (500 Gy doses) from 60Co source. The concentration of pyruvate was estimated by Fermognost tests and the concentration of lactate by Boehringer Mannheim tests. The submaximal physical exercise was found to cause a significantly increased concentration of pyruvate and lactate in the non-radiated and irradiated erythrocytes. Gamma radiation at 500 Gy dose was found to increase concentration of pyruvate in erythrocytes (in the rest and after physical exercise) with simultaneous decrease of lactate concentration.

Adult↗