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J Kemnitz

Publications and source records attributed to J Kemnitz.

At least 55 records · Page 3Linked to original sources
PubMed1989

[In situ hybridization in detecting virus infections in biopsies following orthotopic liver transplantation].

Using the in-situ DNA hybridization we studied 62 liver biopsies from 34 patients for the presence of CMV-, EBV-, HBV-, and HSV-Genome. In the first biopsies positivity-rates were as follows: CMV 62%, EBV 71%, HBV 15%, HSV 15%. In 6 cases a conversion from a negative intranuclear CMV-status to a positive status was seen correlating with an increase in serological titers and with clinical signs of viral infection.

Adolescent↗
PubMed1989

Detection of cells with multidrug-resistant phenotype in myeloproliferative disorders before therapy.

The expression of the multidrug resistance (mdr) phenotype is connected with the overexpression of the P-glycoprotein. By applying the immunocytochemical assay, we have demonstrated that in myeloproliferative diseases (AML, ALL, MDS, CGL) in single cases in smear preparations from the peripheral blood as well as from the bone marrow P-glycoprotein-positive cells, respectively, cells with mdr-positive phenotype can be detected in the material obtained from patients before therapy and without clinically and anamnestically known exposure to cytotoxic or immunosuppressive drugs. In the control group of probands without hematologic disorders and also without clinically or anamnestically confirmed contact with cytotoxic or immunosuppressive drugs, we have found P-glycoprotein-positive subpopulations of cells with positive mdr phenotype in a few cases as well. The uniqueness of our results lies in the fact that this finding demonstrates the presence of subpopulations of mdr-positive cells in leukemias and myelodysplastic syndromes before therapy, and furthermore makes evident that a positive mdr phenotype is not necessarily associated with a malignant phenotype or a malignant cell transformation.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1989

Surgical aspects of familial pheochromocytoma.

During a 15-year-period (1973-1987), 13 patients with familial pheochromocytoma (PCC) underwent unilateral (n = 7) or bilateral (n = 6) total adrenalectomy without major operative morbidity (1 incidental splenectomy in 8 left-side adrenalectomies) and no mortality. 1 patient died 3 years after unilateral adrenalectomy from unrelated cause. The remaining patients were followed up for a mean time of 5.2 years and could be traced and investigated. 3 patients have been adrenalectomised on the contralateral side because of PCC 5, 6 and 10 years after unilateral adrenalectomy. No patient had metastatic PCC. 1 patient sustained recurrent Addisonian crises after bilateral total adrenalectomy. 1 patient developed recurrent hypertension after bilateral adrenalectomy, but without evidence of recurrent disease. In 3 patients bilateral total adrenalectomy with adrenal cortex autotransplantation was performed, however, complete hormone supplementation therapy had to be continued because of autograft functional insufficiency. In considering the number of patients in this study, who only needed a unilateral adrenalectomy in familial PCC, the more pragmatic surgical approach to familial PCC seems to be supported. Bilateral total adrenalectomy with adrenal cortex autotransplantation was not as effective functionally as supposed by histological examination of transplant biopsy.

Adolescent↗
PubMed1988

HLA compatibility and different features of liver allograft rejection.

The influence of human leukocyte antigen (HLA) on acute liver allograft rejection was investigated in 48 adult patients. The diagnosis of rejection was always based on the full triad of histological findings, clinical signs, and the required antirejection treatment. Sixty-two percent of the patients closely observed for 6 months postoperatively revealed acute rejection within the first 3 weeks, mostly on days 7-11. HLA compatibility was not observed to have any significant influence on the incidence of acute rejection. However, different histological and clinical features were revealed in conjunction with DR compatibility. Patients without DR compatibility showed a type of rejection with fever and increase of bilirubin, frequently associated with cholestasis and cholangitis, which sometimes persisted for weeks. Patients with 1 DR compatibility showed a predominant increase of transaminases, which was never associated with cholangitis. The conjunction of different DR compatibilities and various clinical signs may indicate possible pathways from immunological assault to the clinical appearance of acute rejection. A knowledge of a patient's individual compatibility and an expectation of certain rejection patterns may lead to earlier and more reliable diagnosis and treatment.

Adult↗
PubMed1988

[Surgical therapy of sporadic and familial pheochromocytoma].

From 1975 through 1987, 54 patients underwent operations for pheochromocytoma (PCC): 37 patients for sporadic unilateral adrenal PCC, 9 for bilateral and familial PCC, and 4 patients for paraganglioma. In 4 additional patients, laparotomy failed to discover the reason for hypercatecholaminemia. There were no operative mortality and a low morbidity for unilateral (n = 43) as well as for bilateral adrenalectomy (n = 6). To avoid hormone replacement therapy after bilateral total adrenalectomy at least for some years, unilateral instead of bilateral adrenalectomy was preferred to be the initial surgical procedure of choice for familial and MEN-IIA-associated unilateral adrenal PCC. Autologous transplantation of the adrenal cortex were performed in 2 patients after bilateral adrenalectomy. 6 months postoperatively, the transplants were vital as proved by light-microscopy, but sufficiently functioning only in 1 patient.

Adolescent↗
PubMed1987

[Management of rejection following heart transplantation using oral steroid medication].

After 47 orthotopic heart transplantations with long-term immunosuppression (cyclosporin A, 3-10 mg/kg; azathioprine, 1-2 mg/kg; prednisolone 0.1 mg/kg) there were 306 episodes of acute rejection. In 44 of these (14.8%) the oral prednisolone dosage was increased, from a mean of 10.8 +/- 2.95 mg/d to 36.6 +/- 7.2 mg/d, followed by stepwise reduction to the original maintenance dose. Compared with the customary intravenous bolus administration (1.0 g/d for three days: success rate 89%), the oral schedule was successful in 33 of the 44 episodes of rejection (75%). It is concluded that in selected patients acute rejection episodes after heart transplantation can be successful treated by an increase in the oral dose of steroids without changing the dosage of the other two immunosuppressive drugs.

Adolescent↗
PubMed1987

A classification of cardiac allograft rejection. A modification of the classification by Billingham.

We herein propose a classification of rejection in cardiac allografts based on the original Stanford work. Our modified classification, as a work hypothesis, defines the following grades: mild acute rejection (A-1), corresponding to Billingham's "mild rejection"; mild acute rejection with probable conversion to moderate rejection (A-2); moderate acute rejection (A-3), comparable to Billingham's "moderate rejection"; and severe acute rejection (A-4), morphologically identical with the respective grade in the Billingham classification. The resolution of rejection has been classified into two grades--early (A-5a) and late (A-5b) resolution--according to the development of granulation tissues. We also grade the degree of vasculopathy (B-1, B-2) and chronic rejection (C), which is characterized by aggressive fibrosis and persistent vasculopathy. Mild rejection with possible conversion to moderate rejection is defined by an increasing quantity of retrogressive changes in myocytes. Changes not related to transplantation are characterized in our classification by descriptive diagnosis. The proposed classification was validated by 1 year of clinical experience and by the evaluation of possible prognostic aspects of the classification.

Biopsy↗