Biomedical subjects

J Kink

Publications and source records attributed to J Kink.

4 recordsLinked to original sources

PubMed1992

A novel semi-automated paramagnetic microparticle based enzyme immunoassay for hepatitis C virus: its application to serologic testing.

A new rapid serologic enzyme immunoassay for antibodies to hepatitis C virus (HCV) is described. The assay combines synthetic peptide and recombinant antigens representing putative structural and non structural HCV gene products with paramagnetic microparticle assay (MP assay) technology. Assay readout is based upon an enzymatically generated fluorescent product which is quantified with a novel semi-automated washer/reader instrument system. Assay sensitivity and specificity was determined to be greater than the first generation HCV C-100 EIA using a non-A, non-B hepatitis disease panel, an HCV performance panel, an HCV seroconversion panel, dilutions of HCV reactive sera, and random volunteer blood donor specimens.

Evaluation Studies as Topic↗

PubMed1977

Proportional regulation of body form and cortical organelle pattern in the ciliate Dileptus.

Morphometric and ultrastructural studies of shape and pattern regulation were performed on Dileptus anser. In this ciliate 2 body regions can be distinguished: proboscis and trunk. When a large portion of the trunk is excised, shape regulation occurs and the proper proboscis/trunk ratio is restored through elongation of the remnant of the trunk and shortening of the proboscis. When there is nothing but proboscis left, the trunk is formed from the proximal portion of proboscis and again the right proportion is restored. Regulation of the ciliary pattern is based mainly on resorption of some ciliary elements. The resorption of somatic ciliature is especially intense within narrowing regions, where otherwise overcrowding of cilia would occur. The resorption of oral ciliature was found to occur within an area located at the apex of the proboscis. Oral structures when damaged by cutting may be repaired in situ. Changes in the microfibrillar system of the cell were found during regulation of shape of the posterior region of the cell, as well as during regulation of size of oral structures. Regulatory processes in Dileptus are compared to those known for other ciliates, and possible differences between the regulation of shape and the regulation of pattern are discussed.

Animals↗

PubMed1976

A localized region of basal body proliferation in growing cells of Dileptus visscheri (ciliata, gymnostomate).

In the ciliate Dileptus organisms are completely organized at the time of excystation. They grow intensively, increasing in volume several times, before they reach the trophic cell size. The growth includes both the somatic part of the body as well as the oral parts, consisting of the cytostomal field and the ventral band of the proboscis. From the time of excystation, during the growth of the cell, the oral apparatus is able to capture and ingest food. Growth of an oral apparatus while functioning has not previously been reported in cilates. The proliferation of new oral kinetosomes in the growing oral apparatus occurs only in the cytostomal field. There is no proliferation of ciliature in the oral kineties of the growing proboscis. In those regions of the somatic kineties bordering the cytostomal field an extensive proliferation of somatic ciliature takes place. The zone of proliferation common to the oral and somatic ciliature supplies new kinetosomes for the elongating proboscis, the growing oral apparatus and the growth of the rest of the body. The mode of proliferation of the oral kinetosomes differs from that of the somatic kinetosomes. The oral kinetosomes form randomly in large numbers, appearing similar to an anarchic field such as is found in other ciliates. The somatic kinetosomes always arise singly and in a defined position in relation to the mature kinetosome. The proliferation of basal bodies in a defined area in Dileptus suggests the existence of a growth zone localized around the cytostomal field.

Ciliophora↗

PubMedDate not supplied

Improved serologic detection of hepatitis C virus with a paramagnetic microparticle assay using multiple antigenic sequences.

A paramagnetic microparticle (MP) assay for antibody to hepatitis C virus (anti-HCV) was developed, in which the probe for antibody consisted of synthetic peptides corresponding to HCV capsid and nonstructural c-100 regions, as well as a recombinant protein corresponding to the nonstructural c33c region. Assay performance was evaluated by testing serum from 108 geographically diverse patients with non-A, non-B hepatitis (NANBH). The frequency of anti-HCV reactivity detected with the MP assay and with an enzyme-linked immunosorbent assay (ELISA) for c-100 was 91 and 70 percent, respectively. All c-100 HCV ELISA-reactive specimens also reacted on the MP assay. In addition, anti-HCV seroconversion in three plasma donors was detected one to two blood collection dates earlier by the MP assay than by the c-100 HCV ELISA and at similar blood collection dates by the MP assay and a second-generation anti-HCV ELISA. Serologic responses to the three distinct antigenic regions of HCV in NANBH patients varied: reactivity to all three antigens was most common (49%), reactivity to both capsid and c33c (40%) was next most common, and single-antigen reactivity was rare (4%). MP assay reactivity of 825 volunteer donors was 0.1 percent. These results demonstrate both the utility of additional HCV antigens for an effective anti-HCV screening assay and the application of paramagnetic MP technology to serologic testing for HCV infection.

Antibody Specificity↗