PubMed Health⌕ Search

Biomedical subjects

J Klimek

Publications and source records attributed to J Klimek.

At least 73 records · Page 4Linked to original sources
PubMed1980

Inhibition by fatty acids of the cholesterol side-chain cleavage in the course of progesterone biosynthesis in the mitochondrial fraction of human term placenta.

1. Saturated and unsaturated fatty acids inhibit the progesterone biosynthesis from cholesterol in the human placenta mitochondrial fraction. 2. Inhibition of the progesterone biosynthesis by fatty acids could be relieved by ATP, CoA and carnitine. 3. The inhibitory effect of fatty acids is due to their action on the cholesterol side-chain mixed-function oxidase system but not on the NADPH-regenerating system.

Cholesterol Side-Chain Cleavage Enzyme↗
PubMed1979

The relationship between energy generation and cholesterol side-chain cleavage reaction in the mitochondria from human term placenta.

1. The interrelationship between progesterone (from cholesterol) biosynthesis and oxidative phosphorylation in human placental mitochondria was examined. 2. ADP and ATP stimulated the malate, succinate and alpha-ketoglutarate-supported progesterone biosynthesis probably via the energy-dependent pyridine nucleotide transhydrogenase activation. The effect of ADP was abolished by rotenone and antimycin in the presence of malate or alpha-ketoglutarate. 3. In the non-energized state of mitochondria malate may supported progesterone biosynthesis by the malic enzyme-dependent pathway. 4. The inhibitory effects of antimycin or cyanide, and the stimulatory effect of rotenone on the succinate-supported progesterone biosynthesis indicate that the succinate to malate conversion is a necessary condition for the stimulation of progesterone biosynthesis from cholesterol. 5. alpha-Ketoglutarate plus malonate did support progesterone biosynthesis also in the presence of ADP or ATP and to a lesser degree in the presence of DNP and rotenone. Arsenate in the presence of alpha-ketoglutarate, malonate, dinitrophenol and rotenone did not affect significantly progesterone biosynthesis. These results indicate that NADPH may be generated also by a non-energy-dependent transhydrogenation in placental mitochondria.

Adenosine Diphosphate↗
PubMed1979

Penetration of cephapirin and cephalothin into the right atrial appendage and pericardial fluid of patients undergoing open-heart surgery.

To prevent infection in 27 patients who underwent coronary artery bypass or cardiac valve replacement surgery, each patient received a single 2-g dose of either cephalothin or cephapirin intravenously before the operation (prior to opening of the chest cavity). Samples of the right atrial appendage, pericardial fluid, and serum were obtained at various intervals after injection of the antibiotic and were assayed for cephalosporin concentrations. Cephapirin consistently reached higher levels than cephalothin in the right atrial appendage and pericardial fluid; both cephalosporins, however, reach concentrations in these sites well above their minimal inhibitory concentrations (MICs) for penicillin-resistant staphylococci. Of particular interest was the brevity of the period (about 100 min) during which levels of both antibiotics were maintained above the MIC in the right atrial appendage. This finding emphasizes the need for administration of these antibiotics shortly before surgery.

Adult↗
PubMed1978

Bacteraemia after manipulation of the urinary tract. The importance of pre-existing urinary tract disease and compromised host defences.

The factors related to the occurrence of bacteraemia following urinary tract manipulation were studied in a large community hospital. During a 3-year period, forty-six of 326 episodes of hospital-acquired bacteraemia were associated with urinary tract manipulation. All thirty of forty-six cases felt to be definitely related to urinary tract manipulation (other obvious sources of bacteraemia being absent) had pre-existing urinary tract disease, especially of an obstructive type; only one in this group died from sepsis. The remaining sixteen patients had other possible sources of bacteraemia besides urinary tract manipulation and had disorders associated with defects in host defences; twelve (75%) in this group died from overwhelming sepsis. Thus, if bacteraemia occurs in a patient having had urinary tract manipulation but without any underlying urinary tract abnormality or impairment in host defences, its source should be searched for in other areas of the body.

Bacteriuria↗
PubMed1978

Failure of disposable domes to prevent septicemia acquired from contaminated pressure transducers.

Between January and June 1976, eight cases of primary bacteremia due to Enterobacter cloacae occurred in patients after open-heart surgery in a hospital in Connecticut. Epidemiologic studies implicated radial arterial catheters as the route of acquistion of E cloacae, and bacteriologic studies confirmed arterial pressure transducers as the sources of bacteria. Prospective studies indicated that the disposable domes from the pressure transducers did not prevent the spread of bacteria from contaminated transducers to the arterial catheters. This is the first report of transducer-acquired bacteremic infections occurring with the use of disposable domes. Although disposable domes may decrease the chances of cross-contamination of circuits for monitoring pressure, they do not, as previously thought, eliminate the risk of bacteremia from this source.

Blood Pressure↗
PubMed1976

Regulation of progesterone biosynthesis in human placental mitochondria by Krebs cycle metabolites.

1. 2-Oxoglutarate, succinate, fumarate, malate and citrate, cis-aconitate and isocitrate stimulate conversion of cholesterol to progesterone in human placental mitochondria. 2. The stimulatory effect of dicarboxylic and tricarboxylic acids depends on the activity of malate dehydrogenase (decarboxylating) (NADP+) (EC 1.1.1.40) and isocitrate dehydrogenase (NADP+) (EC 1.1.1.42), respectively.

Aconitic Acid↗