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J Kneiflová

Publications and source records attributed to J Kneiflová.

13 recordsLinked to original sources
PubMed1992

[Use of markers of hospital strains in studying the occurrence of nosocomial infections].

The submitted paper deals with one of the possible aspects of the investigation of nosocomial infections, i.e. investigations of the properties of hospital strains, their importance and possible use. The incidence of bacterial hospital strains was investigated in a surgical department of a district hospital of the North Bohemian region in October 1990; at the time of the survey 29 nosocomial infections (36.1%) were revealed by the prevalence method. Bacterial hospital strains were isolated from patients with a nosocomial infection, from the attending staff and the hospital environment. The following markers were investigated: biotyping, phagotyping, serotyping, toxin production, sensitivity to antimicrobial substances and sensitivity to disinfectants. Based on results of marking of hospital strains 7 incidences with a possible epidemiological association were detected where the hospital strain of equal or very similar properties dominated. The results of the investigation confirmed the important participation of the attending staff in the spread of nosocomial infections, in particular via contaminated hands, and drew attention to shortcomings as regards adherence to the hygienic and epidemiological regime in the investigated department.

Bacteria↗
PubMed1992

[Microbicidal effect of Lautercide, a new disinfectant].

The authors tested the effect of a new disinfectant Lautericide produced by Qualichem Co. Neratovice which contains as the active agent acetate amine of coconut acid. For experiments standard methods were used and standard strains for testing of the following microbial strains: St. aureus, E. coli, Ps. aeruginosa, Proteus vulgaris, Serratia marcescens, Candida albicans, spores of Bacillus subtilis, M. tuberculosis, M. fortuitum, M. avium. As a model virus E. coli bacteriophage OX 174 was used. In concentrations from 0.04% to 0.5% and exposures from 2 to 10 minutes Lautercide exerted a bactericidal and fungicidal action. On spores of B. subtilis the preparation was ineffective even in a 10% concentration and 32-minute exposure. Devitalization of mycobacteria occurred after 15 min. action of Lautercide on M. tuberculosis and M. fortuitum, in M. avium in a 10% concentration in 60 min. Lautercide is ineffective against small resistant viruses without an integument. It is recommended to use Lautercide in a 1% aqueous solution for a one-stage disinfection combined with cleaning in the health services, veterinary medicine and in the field of communal hygiene. Before cleaning objects contaminated with infectious material, 5% Lautercide with 30 min. exposure is recommended. In disinfection of objects contaminated with mycobacteria it is recommended to use a 5% solution with 10-minute exposure for M. tuberculosis and M. fortuitum and a 10% solution for M. avium with 60-minute exposure.

Acetamides↗
PubMed1991

New disinfectants.

The authors estimated the microbicidal effect of disinfectants produced by the firm Henkel Austria, namely Spitaderm, Incidin M spray, Incidur, Incidur spray, Sekusept pulver, Sekusept forte and Maranon H. The aim of the study was the verification of the effectivity of preparations and the proposal of their application in Czechoslovak practice. The estimation was performed in two stages: in vitro and in practice. The standard suspensive method, the suspensive micromethod and the method of carriers were chosen for the experiments, as well as the strains of grampositive and gramnegative bacteria, the spores of B. subtilis, yeasts and the model virus. In the second part of experiments, the disinfectants were applied according to the producer's instructions in inpatient departments of internal medicine and of surgery at the University Hospital in Olomouc in the course of 4 weeks. During the experiment, the rubdowns were taken twice a week from the working places, floor, instruments and hands and the bacterial contamination of the establishment was estimated. In hemodialysing centre, the apparatuses of the artificial kidney were estimated as well, as the preparation Maranon H is recommended for their disinfection. The results of the experiments show that the recommended concentrations of disinfectants and their effective exposition are chosen with sufficient safety coefficient. In chosen wards, the minimum bacterial contamination was observed. All disinfectants were accepted favourably, their scent estimated as pleasant, the staffs evaluated their high cleaning properties and the dosage equipment, ensuring the precise concentration of preparations. With regard to the above mentioned results, the authors recommend to start the licensed production, as all the tested preparations correspond to the exigent demands of modern and effective disinfection.

Bacteria↗
PubMed1990

[The effect of glutaraldehyde on mycobacteria and on selected types of bacteria].

The author tested the action of 2% alkalinized glutaraldehyde on Mycobacterium tuberculosis, M. kansasii, M. avium, M. xenopi and M. fortuitum, as well as Staphyloccocus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Bacillus cereus, B. megatherium and B. subtilis. After 5-minute exposure devitalization of M. tuberculosis, M. kansasii and vegetative forms of the tested bacteria occurred. A sporicide effect was recorded already after 30 minutes or after more than 120 minutes. Most resistant were potentially pathogenic M. fortuitum, M. xenopi and M. avium where the disinfectant effect (a drop of CFU by 99.99%) was recorded after 7-63 minutes and their complete devitalization occurred after 3 (M. fortuitum) to 20 hours' (M. avium) action. The author recommends therefore exposure for 24 hours for sterilization of objects contamined with the above species of mycobacteria. This pertains in particular to instruments which cannot be sterilized by heat (endoscopes, bronchoscopes, sigmoidoscopes etc.), used in the diagnosis of mycobacterioses in patients with compromised immunity, e.g. in AIDS.

Escherichia coli↗
PubMed1983

Assessment of viral disinfection by means of bacteriophage OX 174.

A method was developed for evaluating the viricidal effectiveness of chemical disinfectants. The method uses the DNA bacteriophage phi X 174 (ATCC 13706-B1), whose host is Escherichia coli C (ATCC 13706-1), as the model virus. The bacteriophage has particles of icosahedral symmetry, 26 nm in diameter, and resembles animal entero- and parvoviruses by its resistance to physicochemical factors. Disinfection effectiveness was evaluated in suspension medium and on carriers of different materials that are disinfected in practice. The Horvath-Alföldi method on plexiglass panels containing 21 dishes of 40-mm diameter and 2-mm depth was used. A volume of 0.2 ml of phage suspension or one flat carrier 25 mm X 25 mm was put per panel dish and overlaid with 3 ml of dissolved soft agar medium containing at 37 degrees C, quantitatively as VE = log (No/Nd) in suspension tests and semiquantitatively on a 0-5 scale in tests on carrier materials. The viricidal effect of disinfectant-containing washing agents was tested on textile carriers during washing in an experimental washing machine. The method can be adjusted to evaluate the viricidal effectiveness of disinfection under different environmental conditions, e.g. in sanitary, agricultural, veterinary or food-industry facilities.

Bacteriophage phi X 174↗