PubMed Health⌕ Search

Biomedical subjects

J Knight

Publications and source records attributed to J Knight.

34 records · Page 2Linked to original sources
PubMed1985

Immunocytochemical identification of cell types in pleomorphic adenoma, with particular reference to myoepithelial cells.

An immunocytochemical study was carried out on normal salivary gland tissue and ten salivary gland pleomorphic adenomas. Antibodies to myosin were used to stain myoepithelial cells. Duct cells were stained using an antibody to total keratin and a subpopulation of basal duct cells with an antibody to 45/46K keratins. Basement membranes were stained with anti-type IV collagen. The results demonstrated that myoepithelial cells are relatively rare in the majority of pleomorphic adenomas and that many of the cells which have been classically described as myoepithelial in routine histological preparations do not clearly show this type of differentiation. However, the tumors presented a spectrum of differentiation patterns from those that were mainly ductal to the rare tumour which was largely myoepithelial. It is further suggested that the 45/46K keratin antibody is capable of identifying a subpopulation of cells which could possibly be important in the histogenesis of this tumour.

Adenoma, Pleomorphic↗
PubMed1978

Peer review.

Explore the source record for details and available documents.

Australia↗
PubMed1978

Medical manpower.

Explore the source record for details and available documents.

Australia↗
PubMed1978

Radioimmunoassay and radioenzymatic assay of a new aminoglycoside antibiotic, netilmicin.

A radioimmunoassay and a radioenzymatic assay for netilmicin, a new aminoglycoside, were developed in our laboratories to assist in the study of the pharmacology of the drug and to establish values for use in its monitoring. The assays are sensitive, precise, and rapid, giving results that correlate (r = 0.90) with each other and with those of a microbiological assay in which Klebsiella pneumoniae is used as the test organism. Preliminary pharmacological studies show the drug to have a biological half-life of 135 min. which is comparable to that for other aminoglycosides.

Biological Assay↗
PubMed1976

Effects of leached mirex on experimental communities of estuarine animals.

Experimental communities of various estuarine animals in outdoor tanks were exposed to a continuous flow of water containing mirex for 10 weeks. The mirex was leached from fire ant bait (0.3% active ingredient) by fresh water which was then mixed with salt water to yield exposure concentrations averaging 0.038 mug/L. The experiment simulated runoff from treated land into estuarine areas. Mortality of grass shrimp (Palaemonetes vulgaris), pin, shrimp (Penaeus duorarum), common mud crabs (Panopeus herbstii), and striped hermit crabs (Clibanarius vittatus) was significantly high in tanks containing the toxicant. Mortality of ribbed mussels (Modiolus demissus) and American oysters (Crassostrea virginica) was significantly lower in treated tanks, probably because numbers of both species of crabs, which ate the bivalves, were reduced. Sheepshead minnows (Cyprinodon variegatus) were least affected by mirex. Almost all deaths occurred after 10 or more days of exposure. All exposed animals accumulated mirex, with maximum concentrations ranging from 5,500X (pink shrimp) to 73,700X (soft tissues of oysters) above the concentration in the water. Sand substratum contained mirex up to 1,500X that in the water. The study demonstrated that mirex can be leached from bait by fresh water and concentrated by and affect survival of members in an experimental estuarine community.

Animals↗
PubMedDate not supplied

A child-to-child programme in rural Jamaica.

A child-to-child programme was carried out in six rural schools in Jamaica. School children in grades 4 and 5 were taught concepts of nutrition, environmental and personal hygiene, and child development. The children were encouraged to take messages home to their parents and improve the care of their younger siblings. Four hundred and twenty-three children and 90 mothers and guardians from four of the project schools were evaluated and compared with 199 children and 47 parents from two control schools. The project children showed higher scores on a test of knowledge in the topics at the end of the year. They also improved in some practices at home. The mothers improved in knowledge in some areas but not in their practices. We consider the results sufficiently encouraging to justify incorporating the programme into the country's education curriculum for primary schools.

Adult↗
PubMedDate not supplied

Lipoperoxides in sebum of substance users and controls.

Sebum was collected from forehead skin in five compulsive heroin and/or cocaine (substance) users and in five controls over three consecutive periods, each lasting three hours. The participants were adult black and white men similar in age and smoking habits, who did not consume alcohol. Lipoperoxides were determined in sebum as malondialdehyde by high performance liquid chromatography. Two participants were excluded in the control group: in both, urinary lipoperoxides were elevated; in one, urine tested positive for delta-9 tetrahydrocannabinol (THC). All other participants had negative urine drug screening tests. Relative to the controls, all substance users had elevated concentrations of lipoperoxides in urine. Compared to the controls, the rate of sebum excretion in the last collecting period was higher in substance users, but sebum had significantly lower lipoperoxide concentration. It is assumed that compulsive drug use may influence lipoperoxidation of incipient sebum, possibly by altered tissue perfusion.

Adult↗
PubMedDate not supplied

A radioimmunoassay for tallysomycin.

Antibodies were raised against a new antineoplastic agent, tallysomycin, for use in a radioimmunoassay. The assay was sensitive to 2.5 ng of tallysomycin, had a coefficient of variation of 10.5% at 50 ng/ml, and was highly specific for tallysomycin A. The antibody exhibited reduced cross-reactivity with closely related tallysomycin analogs and little or no immunoreactivity with other antitumor antibiotics including bleomycin and phleomycin. Increased immunoreactivity occurred following copper chelation of tallysomycin B as compared with the non-chelated forms, thus indicating possible conformational alterations upon metal binding.

Antibiotics, Antineoplastic↗