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Biomedical subjects

J Kocher

Publications and source records attributed to J Kocher.

7 recordsLinked to original sources
PubMed1993

The neuropeptide VIP regulates the expression of the tyrosine hydroxylase gene in cultured avian sympathetic neurons.

The neuropeptide vasoactive intestinal polypeptide (VIP) increases the activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in the biosynthesis of catecholamines, in cultured chicken sympathetic neurons. We report here that VIP acts by increasing TH mRNA levels in these cells. Induction of TH mRNA is transient and reaches maximal values 6-8 h after the addition of the peptide to the cultures. TH mRNA levels return to control values after 1-2 days. The quail cDNA probe detects a single mRNA species of approximately 9 kb in RNA extracted both from embryonic chicken sympathetic neurons and adult quail adrenal medulla.

Animals↗
PubMed1992

Mycobacterium haemophilum osteomyelitis in an AIDS patient.

Mycobacterium haemophilum is a fastidious species that has been isolated from skin and subcutaneous nodules. The authors describe a case of Mycobacterium haemophilum osteomyelitis and skin infection in an AIDS patient and successful treatment with minocycline.

Acquired Immunodeficiency Syndrome↗
PubMed1989

Polyendocrine failure with hypogonadism.

Primary gonadal failure, in males with autoimmune endocrinopathies, rarely has been described. We present a unique case of transient hypergonadotropic hypogonadism in a male with type 2 polyglandular failure (Addison's disease and autoimmune hypothyroidism).

Addison Disease↗
PubMed1986

Determination of benzarone in human plasma and urine by high-performance liquid chromatography and gas chromatography-mass spectrometry. Identification of the conjugates.

Benzarone (the debrominated metabolite of the uricosuric drug benzbromarone) has been proposed for treatment of vascular disorders. An assay was developed for the quantitation of total benzarone (conjugated and unconjugated) in plasma and urine, following oral intake of benzarone. Enzymatic hydrolysis of the samples with beta-glucuronidase/arylsulphatase, extraction, gradient elution high-performance liquid chromatography with reversed-phase columns and UV detection were used for the assay. The concentration ranges, precision and sensitivities were: 0.01-2 micrograms/ml, 3-5% and 0.01 microgram/ml, respectively, for both plasma and urine. These results were validated by gas chromatography-mass spectrometry after methylated derivatives were prepared. Enzymatic hydrolysis of plasma with pure beta-glucuronidase or arylsulphatase showed that the relative amounts of unconjugated, glucuronidated, and sulphated benzarone were 6, 12 and 82% respectively, for both plasma and urine.

Adult↗
PubMed1982

[A new indirect binocular spectacle ophthalmoscope].

A new indirect spectacle ophthalmoscope is described. Its main features are a fixed interpupillary distance (59, 62, 65, 68 mm.), a variable viewing beam stereoangle, a precentered halogen lighting beam with Maxwellian view and an iris diaphragm to limit the viewing area, which has never been a feature of a binocular ophthalmoscope before. These features, on a lightweight spectacle frame, make indirect binocular ophthalmoscopy an easy-to-master method even for examining patients with small pupils and hazy media.

Humans↗