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J Kohlícek

Publications and source records attributed to J Kohlícek.

At least 19 recordsLinked to original sources
PubMed1989

Gas chromatography of simple phenols in biological fluids.

Acid hydrolysis of phenol conjugates in urine by concentrated H3PO4 followed by extraction of phenols with n-hexane and their acetylation before gas chromatography on columns packed with OV-1 or OV-17 is described. The sensitivity of the method is sufficient to monitor normal levels of phenol and p-cresol or phenol and o-cresol after exposure to benzene or toluene vapours. The detection limit is 1 mg/l. The method can also be used to diagnose acute oral intoxication by phenol or cresols and to estimate its significance in clinical or forensic toxicology. Normal urine levels of phenols and those in different cases of human intoxication are evaluated.

Chromatography, Gas↗
PubMed1988

Rapid determination of ethylene glycol at toxic levels in serum and urine.

The rapid gas chromatographic detection and determination of ethylene glycol in biological fluids is described. Phenylboronic acid in acetone was used for the esterification of glycol. The phenylboronates of ethylene glycol and 1,2-propylene glycol are not separated on a packed column of medium polarity (OV-17), but they can be separated on a non-polar column (OV-101). In both instances, 1,3-propylene glycol can be used as an internal standard. The method requires only 100 microliters of serum or urine and is suitable for trace analysis in an emergency toxicological laboratory. The utility of the method is demonstrated on two cases of human intoxication with ethylene glycol.

Child↗
PubMed1968

[Urate lithiasis].

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Acetazolamide↗