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Biomedical subjects

J Komárek

Publications and source records attributed to J Komárek.

At least 19 recordsLinked to original sources
PubMed2000

Speciation of copper, lead and cadmium in aquatic systems by circulating dialysis combined with flame AAS.

The assessment of free Cu(II), Pb(II) and Cd(II) ions in the presence of complexed species was realised by a circulating dialysis with Cuprophan planar membranes and subsequent quantification by flame atomic absorption spectrometry. The effect of the flow rate, the time of equilibration, pH and the presence of various complexing agents in the donor solutions were studied. The determination of free Cu(II), Pb(II) and Cd(II) ions in the presence of soil humic substances resulted from the above studies.

Journal Article↗
PubMed1996

Determination of cadmium and copper with ET-AAS after electrochemical deposition on a graphite electrode.

The electrodeposition of cadmium and copper on a special graphite disk electrode has been performed at controlled potential. The electrode with the deposit has been inserted into the graphite atomizer HGA-400 by an adapted automatic sampler for the final determination by ET-ASS. The sensitivity of determination has been 0.371 (microg l(-1))(-1) for cadmium and 0.025 (microg l(-1))(-1) for copper for 2 min electrodeposition and increased linearly with the time of deposition. The limit of detection (3s(bl)) has been 7.9 ng l(-1) Cd(2+) and 0.11 microg l(-1) Cu(2+) for 2 min deposition and it has been improved with increased time of electrodeposition. The technique has been applied to the determination of both metals in seawater and to speciation in the presence of EDTA complexing agent.

Journal Article↗
PubMed1996

Dynamics of some biochemical indices in myocardial infarction treated with thrombolysis and creatine phosphate.

There was studied the effect of intravenously administered creatin phosphate (Neoton-drug with cardioprotective effect, Alfa Schiapparelli Wassermann) in the group of 98 patients affected by their first attack of myocardial infarction. In the course of the treatment there were observed the serum level of many biochemical indices, including the cardioselective enzyme activity. We concluded that the patients treated with Neoton, compared with the control group, revealed the significantly higher elevation of the CK, AST and LDH activity levels and the shift of the CK peak activity towards to the earlier hours after the onset of anginal pain.

Female↗
PubMed1993

[The effect of creatine phosphate (Neoton) in acute myocardial infarct (a prospective multicenter pilot study].

The authors investigated in a group of 106 patients with a first myocardial infarction treated by thrombolysis the effect of i.v. administration of creatine phosphate (a new drug with cardioprotective action--Neoton Alfa Schiapparelli Wassermann Co). In the course of treatment electrocardiographic changes were recorded and the presence of arrhythmias examined by the Holter technique. The Neoton group displayed a statistically insignificant but nevertheless obvious trend of electrocardiographically less serious forms of infarction and a lower incidence of infarctions, as compared with the control group.

Female↗
PubMed1992

[Creatine phosphate as a drug in acute myocardial infarct].

The reserves of endogenous creatine phosphate are very rapidly exhausted in ischaemia. This leads at first to reversible and later to irreversible cell damage. Creatine phosphate can be administered in the preparation Neoton. It serves not only as a source of energy after arrest of oxidative phosphorylation; its main effect is stabilization of sarcolemma and protection against its disintegration. Several publications provide evidence of its favourable effect on the protection of the heart muscle during operations on the open heart, on a reduced incidence of ventricular arrhythmias in acute myocardial infarction and improvement in chronic cardiac insufficiencies. In Czechoslovakia multicentre clinical studies are under way testing Neoton in cardiological indications.

Animals↗
PubMed1990

[Changes in the extent of residual stenosis in coronary arteries after thrombolytic therapy in acute myocardial infarct].

The extent of residual coronary artery stenosis has been studied immediately after thrombolysis using streptokinase and after 3.-4. weeks follow-up in 28 patients with acute myocardial infarction. Thrombolysis was successful in 19 patients (67.9%). In these patients the extent of residual coronary artery stenosis (percentage narrowing of diameter) decreased from 73.4% to 66.1% (p less than 0.05). The residual stenosis did not change in 5 patients (26.3%), increased in 1 patient (5.2%), 3 patients suffered reocclusion of the vessel (15.8%). Marked improvement was found in 10 patients (52.6%). It means despite significant regression a coronary artery stenosis of more than 50% of diameter persisted in 47.4% of patients with successful thrombolysis 3.-4. weeks after the infarction. The factors which could be involved in regression of residual stenosis of vessel related to myocardial infarction are discussed.

Coronary Angiography↗
PubMed1990

[Relation of angina pectoris before myocardial infarct to coronarographic findings after thrombolytic therapy].

Patients with significant residual stenosis after thrombolytic therapy during acute myocardial infarction have higher risk of reinfarction, periinfarction ischemia and sudden death. Early revascularisation can prevent such complications. To find out patients who are at increased risk and are candidates for early angiography 74 patients undergoing angiography after thrombolytic therapy were reviewed. Significant residual stenosis (greater than or equal to 50% diameter reduction) of infarct related artery was present in 85%, high grade stenosis (greater than or equal to 75%) in 58% of patients respectively. The group of patients with preinfarction angina (Canadian class I to IV. 24 hours before infarction) had higher mean residual stenosis than patients without angina (76.5% vs 64%). There was significant difference in presence of preinfarction angina in patients with high grade stenosis and without high grade stenosis (79% and 21% respectively). Thus preinfarction angina can be used to identify patients with higher risk of reocclusion after thrombolytic therapy during acute myocardial infarction. Early coronary angiography and revascularisation can prevent serious complications in these patients.

Angina Pectoris↗
PubMed1989

[Percutaneous transluminal coronary angioplasty. Initial clinical experience].

Percutaneous transluminal coronary angioplasty was performed in 20 patients with angina pectoris (16 patients with stable AP, class II-III., 4 patients with unstable AP) in the period of 1 year. There was single-vessel disease in all patients. Left ventricular function was normal as well in all patients. PTCA was successful in 16 patients (80%), in the remaining 4 patients coronary artery stenosis was not reached with balloon catheter. There were no death, Q-wave myocardial infarction or emergency bypass surgery. Transient ischemic changes on ECG has been found in 2 patients (10%). All patients with successful PTCA were symptomatically improved in period of 1-12 months, in one half of them this improvement was confirmed with stress ECG. Control coronary angiography was made in 2 patients until now, restenosis was not found.

Adult↗
PubMed1989

[Dermatophytes in clinically healthy dogs and cats].

The hair and skin of 300 clinically healthy animals, 268 dogs and 32 cats, were examined mycologically. The method described by Mariat and Tapia (1966) was used for the examination, along with square pieces of the fitted carpet Kovral. The dermatophytes were isolated in 12 samples, all from the material taken from dogs. Trichophyton mentagrophytes was isolated six times, Microsporum canis four times, Trichophyton gallinae once, Trichophyton rubrum once. The origin of the dermatophyte Trichophyton gallinae, found in a village dog, was not determined. In one case of occurrence of the dermatophyte Trichophyton mentagrophytes in a dog the dermatophyte was probably transmitted from man to the dog. Microsporum canis was not proved to be the most frequent dermatophyte in dogs or cats in this country.

Animals↗
PubMed1985

[Low-fat milk syndrome].

Three groups of cows--one group given linseed supplement, the second split rations of concentrates and the third as control--were studied for five months to investigate the biochemical parameters of rumen fluid and blood serum in animals with the "low-fat milk syndrome". The lowest decrease of butterfat content was observed in the linseed-supplement group of cows. In this group the lowest drop of acetic acid in rumen was found out, along with an increase in adipose metabolites in blood serum--total lipids, triglycerides and cholesterol. There were no variations of the content of glucose and total protein in blood. It has been stated that a linseed supplement can have a positive influence on the "low-fat milk syndrome".

Animal Feed↗
PubMed1983

[Biochemical and hematological values in the blood of the nutria].

Sixty-seven clinically healthy mature coypus were studied for some biochemical values in blood, 25 coypus were examined for the distribution of serum proteins and 20 ones for haematological values. Blood was sampled when the animals were killed and was examined by current laboratory methods. In comparison with the other animals, coypus had higher values of phosphorus and magnesium, and lower values of total protein and gamma globulins. The activity of enzymes (AST, ALT, GMT, ALP) was at the same level as in the other animals. Lower values of haemoglobin were found in coypus; the remaining haematological values are close to those in the other animals.

Animals↗
PubMed1981

[Vaccination of cattle against trichophytosis in the prevention and control of dermatophytic zoonoses].

The results of a four-year study of the occurrence of trichophytosis in some cattle stocks in the Pardubice district are presented. The monoetiological nature of this mycosis is documented; this mycosis can be successfully controlled by vaccination. Experiments demonstrated a good activity of antigens which were included in commercial vaccines. The evaluation of the antibody reaction in test rabbits and in vaccinated and spontaneously infected cattle was subjected to critical analysis. However, the main point of the whole study is a general evaluation of the possibilities and limitations of cattle vaccination against trichophytosis as ensues from literary data and from the authors' experience. The results indicate that in permanent application of all the hitherto used preventive and repressive methods a technically correct active immunization of cattle means the best contribution to the control of trichophytosis. The discussion deals with the theoretical problems of the nature and duration of immunity to these diseases and with the choice of the best vaccines and vaccination procedures.

Animals↗
PubMed1978

[Biochemical and hematological changes in the blood of horses after the "Velká Pardubická" steeple chase].

Blood parameters were studied in two groups of horses in the "Velká Pardubická" steeple-chase in 1974, 1975 and 1976. After the race, the levels of lactate showed a manifold increase; an increase was also ascertained in the levels of glucose, sodium, potassium, haemoglobin, in the haematocrit value and in the number of erythrocytes. The following parameters significantly dropped: the levels of acid-base balance - pH, base excess, bicarbonate levels. It was proved that the values of the same parameters in horses during training were incomparably lower. It is advisable to examine horses thoroughly during training and to use the results of training for the evaluation of their condition before difficult races.

Animals↗