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Biomedical subjects

J Konrad

Publications and source records attributed to J Konrad.

7 recordsLinked to original sources

Serodiagnosis of Babesia motasi (Wales), Theileria recondita (Wales) and Cytoecetes phagocytophila infection in sheep.

The indirect fluorescent antibody test (IFAT) was used to diagnose some tick-borne infections of sheep, Babesia motasi (Wales), Theileria recondita (Wales) and Cytoecetes phagocytophila. Antigen was prepared from blood derived from splenectomised sheep except for C phagocytophila which was derived from a normal animal. A field survey was made to assess the prevalence of B motasi and T recondita in North Wales and a comparison made between the titres using the B motasi (Wales) antigen with those previously reported. IFA titres reported in the homologous system were consistently lower than those described previously. The results of the field survey suggested that B motasi (Wales) infection is more widespread than was originally thought and more widespread than the known distribution of its vector Haemaphysalis punctata. No serological cross reactions occurred between B motasi (Wales), T recondita (Wales), C phagocytophila, B divergens, Sarcocystis ovicanis and Toxoplasma gondii.

Animals

Maintenance of in vitro cultures of Babesia divergens and Babesia major at low temperatures.

Cultures of erythrocytes parasitized by Babesia divergens and Babesia major were stored in medium cooled to 4 degrees C for up to 8 weeks. There was a marked decrease in parasitaemia and an increase in the number of free extra-erythrocytic, unagglutinated merozoites, during the cooled period. Cultures stored in this way and returned to 38 degrees C resumed growth, with or without sub-culture. At the low temperature, only one sub-culture is required per week.

Animals

[Incidence and clinical importance of Haemophilus vaginalis (Corynebacterium vaginalis) (author's transl)].

In 250 women aged between 16 and 55 years who came to a gynaecologist we looked for haemophilus vaginalis. In 9.6% it was possible to culture haemophilus vaginalis. The comparison of the differential vaginalis (V-)medium of Greenwood with the starch medium of Smith showed the much better resolution of the Greenwood medium. In one-half of the patients with haemophilus vaginalis there was no clinical evidence of pathogenicity of the bacterium, whereas in the other half, there was only a slight symptomatology. Haemophilus vaginalis and lactobacillus acidophilus seem to exclude each other. The strains of haemophilus vaginalis were resistant to sulfonamides, polymyxin B and aminoglycoside antibiotics.

Adolescent

Some risk factors in pathologic aging.

A clinico-epidemiologic prevalence survey was conducted in the sub-mountainous area of Northern Bohemia, which is distinguished by the highest proportion of aged people in Bohemian countries. It involved 6057 persons of the 70-79 age group, 1718 persons of the 80-89 age group and 139 persons of the 90+ group, plus control subjects in various other groups. In this study an attempt was made to find an effective measure of the presumed risk factors of pathologic aging in persons of the 70-105 age group. The data were analyzed by the method of coincidence of two parameters of binomial decomposition on the computer Odra. The quantity dependence of the signs was determined, and an index of the compound-risk factor of pathologic aging was calculated. When the numerical value of the index is lower than 56.9 +/- 4.36 for a group of males or 50.3 +/- 3.55 for a group of females, the probability is that a given person in those groups will survive to an advanced age. The total risk is higher for the male sex. Of considerable importance are the genetic burden, external environmental causes, harmful habits, certain "civilization" diseases, and other factors such as disturbed social contacts and defective nutrition.

Aged

[Reconstruction of the lacrimal canaliculi (author's transl)].

The different methods of reconstructing obliterated lacrimal canaliculi are described. At the Eye Clinic in Erlangen Henderson's method is prefered. Into a new canal between the lacrimal punctum and the lacrimal sac a tube of polyethylene is introduced and remains there for some weeks. About 60% of the reconstruction lacrimal canaliculi were still open after months and years.

Drainage