Moulting hormone in Locuta migratoria: rate of ecdysone 20-hydroxylation and excretion during the last larval instar.
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Animals↗
Biomedical subjects
J Koolman
Publications and source records attributed to J Koolman.
[The role of the prothoracic glands in the production of ecdysone during the last larval instar of Locusta migratoria L].
Tritiated cholesterol is rapidly converted to labelled ecdysone in vitro by prothoracic glands from last instar larvae of Locusta at the time of the maximum endogenous hormone increase of the insects. Glands from larvae with low hormone content or fat body fragments do not make similar conversions.
Animals↗
Ecdysone Oxidase, an enzyme from the blowfly Calliphora erythrocephala (Meigen).
In the blowfly, the formation of 3-dehydroecdysone from the insect molting hormone ecdysone is catalyzed by an enzyme which carries hydrogen from ecdysone and ecdysterone to oxygen. The enzyme is therefore called "ecdysone oxidase". Two methods are described for the detection of ecdysone oxidase activity, one using a radiolabelled substrate which is separated from the product by thin-layer chromatography after the reaction, and the other using dichloroindophenol, which is discoloured by the redox reaction. The ecdysone oxidase is purified by a factor of 2200 from prepupae of Calliphora erythrocephala using salt precipitation and ion exchange chromatography. The ecdysone oxidase has a Km value for ecdysone of 42muM. The pH optimum is 6.5. The temperature optimum lies at 45 degrees C. The ecdysone oxidase has a molecular weight of 240000.
2,6-Dichloroindophenol↗
Molting hormone titer and metabolic fate of injected ecdysone during the fifth larval instar and in adults of Locusta migratoria (Orthoptera).
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Age Factors↗
Prothoracic glands in the regulation of ecdysone titres and metabolic fate of injected labelled ecdysone in Locusta migratoria.
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Animals↗
[Physiological significance of the metabolism of ecdysone in insects (author's transl)].
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Animals↗
Sulphage esters as inactivation products of ecdysone in Locusta migratoria.
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Animals↗
Reactivity of some transition metals on nuclear protein biosynthesis in rat liver.
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Aluminum↗
[Protein biosynthesis in rat liver cell nuclei].
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Adenosine Triphosphate↗
Quantification of ecdysteroids by immunoassay: comparison of enzyme immunoassay and radioimmunoassay.
The performance of enzyme immunoassay (EIA) and radioimmunoassay (RIA) in the quantitative analysis of ecdysteroids was compared. The EIA was found to be at least equivalent to the RIA with respect to analytical range and sensitivity and to be more comfortable with respect to safety and time saving. When biological samples were analyzed by both assays a good correlation (r = 0.83) was found. Since the EIA has certain advantages over the RIA, we now recommend the use of the former assay for the quantification of ecdysteroids.
Animals↗