PubMed Health⌕ Search

Biomedical subjects

J Kuźniar

Publications and source records attributed to J Kuźniar.

At least 19 recordsLinked to original sources
PubMed2004

Oxidative stress and coenzyme Q10 supplementation in renal transplant recipients.

Recently some reports about the oxidative stress in renal transplant recipients have been published. The role of coenzyme Q10 (CoQ10) as radical scavanger is largely known. The aim of our study was to evaluate the protective role of CoQ10 in renal transplant recipients on lipid peroxidation and lipids parameters, as well as its influence on antioxidant enzymes, neutrophils chemiluminescence and urinary enzymes. The study was performed in 11 long term allograft recipients treated additionally with CoQ10 90 mg/day in three doses, 30 mg each for four weeks. The malonyldialdehyde (MDA) and 4-hydroxynonenal (4-HNE), superoxide dismutase (SOD) and glutathione peroxidase (GPx) and the basic parameters of lipid metabolism such as total cholesterol (TC), high and low density lipoproteins (HDL, LDL), triglycerides (TG), atherogenicity indicators [LDL/HDL; (TC-HDL)/HDL] were evaluated. The chemiluminescence of neutrophils (luminol, fLMP-method) were mesured and the activity of N-acetyl-beta-D-glucosaminidase (NAG), alanylaminopeptidase (AAP), elastase, alpha-1-antitrypsin. All parameters were estimated before and after CoQ10 treatment. Statistically significant changes were noticed with the LDL and atherogenicity indicators (p < 0.01) (decrease) as well as HDL level (p < 0.001) (increase). Also the significant decrease of fMLP stimulated PMNL chemiluminescence (p < 0.05) confirms the antioxidative properties of CoQ10. The significant increase of NAG activity (p < 0.05) can't be the result of nephrotoxic effect, because NAG-B is unchanged. Serum concentration of creatinine and cyclosporine A in renal allograft recipients was unchanged after CoQ10 treatment. The presented date shows that further study with CoQ10 treatment in renal transplant in larger numbers and over longer periods should be considered.

Acetylglucosaminidase↗
PubMed2001

Enzymuria and beta2-mikroglobulinuria in the assessment of the influence of proteinuria on the progression of glomerulopathies.

The important role of the tubulo-interstitial system for the progression of glomerulonephritis (GN), is the cause of a continuous search for the proper markers of kidney tubules damage, which can be applied in clinical diagnosis. In the present work the activity of N-acetyl-beta-D-glucosamidase (NAG), its isoenzyme NAG-B, alanylaminopeptidase (AAP), gamma-glutamyltransferase (GGT), concentration of beta2-microglobulin (beta2M) and daily protein excretion in the urine of 37 patients with morphologically different glomerulopathies were measured. The serum creatinine was also controlled. The obtained results suggest that activity of NAG in the patients with GN has an intermediate connection with proteinuria and could be a cause of the inflammatory process of the kidney, but the activity of AAP is directly dependent on urine protein concentration. Systemic analysis of both partial and multiple correlation coefficients of the examined indicators creates new, additional possibilities in the estimation of activity and progress of GN.

Acetylglucosaminidase↗
PubMed1999

Diagnostic application of AAP isoenzyme separation.

We describe the separation of alanylaminopeptidase (AAP) from urine into two isoenzymes: a particulate and a soluble form. The separation was accomplished using ion-exchange column chromatography on DEAE-52 cellulose. The purity of the isolated forms was confirmed electrophoretically. We attempted to create a method allowing the quantitative assessment of AAP isoenzymes in urine based on electrophoretic separation in 7.5% polyacrylamide gel with subsequent densitometric analysis. The content of AAP isoenzymes in examined urine was estimated using ultracentrifugation. The differences in the content of cytosolic and microsomal forms were observed suggesting the possibilities of using AAP isoenzymes in diagnostics. Furthermore, the effect of temperature on the activity of AAP separated on DEAE-52 cellulose was studied.

CD13 Antigens↗
PubMed1999

Enzymatic markers of cyclosporine nephrotoxicity in patients after renal transplantation.

Toxicity of cyclosporine (CsA), an immunosuppressive drug widely used in transplantation, to the transplanted kidney creates a serious side effect. Therefore, searching for sensitive indicators of nephrotoxic action is well worth the effort. In this work we describe the results of estimation of urine concentration of lysosomal enzymes widely present in the kidney: N-acetyl-beta-D-glucosaminidase (NAG), its isoenzyme NAG-B and beta-glucuronidase (beta-Gr). The studies were conducted in various periods after transplantation of kidneys, on patients under various treatments and receiving different doses of CsA. The results indicate a substantial dependence of the activity of NAG and NAG-B on CsA doses and the period after transplantation. The enzyme proved to be also a sensitive indicator of graft rejection. No such dependence was observed in the case of beta-Gr.

Adolescent↗
PubMed1999

Enzymuria in antibiotic therapy of acute infections.

Activities of N-acetyl-beta-D-glucosaminidase (NAG), its isoenzyme NAG-B, alanylaminopeptidase (AAP), elastase and trypsin inhibitor (alpha 1 PI) were evaluated as markers of nephrotoxicity and inflammation in acute infections treated with various antibiotics (vancomycin, netilmicin, pefloxacine, cefoperazone and imipenem).

Acetylglucosaminidase↗
PubMed1999

[A case of cylindroma of the parapharyngeal space].

The authors show a case of parapharyngeal space cylindroma paying attention to large diagnostic difficulties. This tumor has been characterized by a slow local growth but after five years disease involved many organs with metastases.

Carcinoma, Adenoid Cystic↗
PubMed1996

[Influence of vapours of paint and toxic dusts on mucous membranes of the upper airways in paint and varnish factory workers].

Macroscopic, cytologic and bacteriologic conditions of mucous membranes of the upper airways in workers (n = 146) of the Polifarb Factory, Wrocław, exposed to dusts and solvent vapours used in the paint and varnish production was estimated. In 89% of the workers, pathologic changes in throat mucous membranes were observed. Three types of macroscopic changes were distinguished. In workers with the shortest period of employment, laryngeal oedema congestion alteration was diagnosed, and atrophic changes with medium intensity were observed in workers employed for a long period. It was found that cytologic changes in the nose mucous membrane depended on the duration of exposure. Inflammation cytograms appeared during the first period of exposure to the substances discussed. The longer period of exposure, the more clear features of metaplasia squamous epithelium. The composition of the nose and throat bacterial flora changed according to the length of employment. An increased growth of G(-) genera Enterobacter, Klebsiella, Proteus, Escherichia and Candida fungi was found in workers with long period of employment.

Adult↗
PubMed1994

N-acetyl-B-D-glucosaminidase isoenzymes in the diagnosis of poisoning and kidney diseases.

Damaged lysosomes from renal tubular cells are, to the greatest degree, the source of activity of NAG in urine. Besides this, the enzyme can appear as a result of degranulation of granulocytes (PMN), active infection of the urinary system as also from serum as a result of glomerular filtration during damage to the glomerular basement membrane. For the purpose of explaining the source of origin of the enzyme in urine, NAG was separated into isoenzymes from the kidneys, granulocytes and serum for comparison with isoenzymes in physiological and pathological urines after ethylene glycol poisoning, and glomerulonephritis, respectively. The separation was made by column ion-exchange chromatography on DEAE-52 cellulose and by electrophoresis in 7% polyacrylamide gel. In addition, the thermostability of isolated isoenzymes was compared.

Acetylglucosaminidase↗
PubMed1992

The variety of clinical and histopathologic presentations of glomerulonephritis associated with latent syphilis.

Glomerulonephritis is a well established but rather uncommon complication of latent secondary syphilis. We present three cases of glomerulopathies associated with luetic infection, observed and managed in our institutions in the past three years. They illustrate a variety of clinicopathologic presentations of this nephropathy, from acute nephrotic syndrome through membranous glomerulopathy up to rapidly progressive glomerulonephritis. Regardless of the clinical course and histologic type, they were all characterized by strongly positive results of serologic tests for syphilis. Our observations suggest the necessity of eliminating luetic infection in aetiologic considerations of each newly diagnosed case of nephrotic syndrome.

Adult↗
PubMed1991

Chemiluminescence of neutrophils in patients with glomerulonephritis treated with methylprednisolone.

The effect of high dose methylprednisolone (Solu-Medrol, Up John) (1000 mg) on chemiluminescence (CL) of peripheral blood neutrophils in patients with various primary glomerulopathies was determined. Contrary to the well-established influence of corticosteroids on in vitro suppression of leukocytic chemiluminescence, our studies disclose a remarkable enhancement of neutrophil chemiluminescent activity in patients receiving pulse methylprednisolone infusion. Subsequent administrations of the drug did not result in any significant change of the analysed parameters. Increased chemiluminescence returned to the pretreatment values within approximately seven days. A possible explanation for the observed phenomenon is discussed.

Adult↗
PubMed1990

[Is local antiproteolytic failure the cause of a prolonged course in various forms of glomerulonephritis?].

Just as in other inflammatory processes in glomerulonephritis also a great role has been ascribed recently to proteases released from phagocytes and mesangial cells. On the basis of original observation (clinical and experimental) of glomerulopathy inhibition by EACA, the authors present the concept of local antiproteolytic failure as the cause of protracted course of certain forms of glomerulonephritis.

Aminocaproic Acid↗
PubMed1989

Isoenzymes of N-acetyl-beta-D-glucosaminidase in urine of patients with acute renal failure after intoxication.

Activity of urinary N-acetyl-beta-D-glucosaminidase (NAG) was determined and basic clinical studies were carried out in oliguric, polyuric phases and restoration to health in patients with acute renal failure due to intoxication. At the same time, enzyme separation into isoenzymes A and B was achieved by column ion-exchange chromatography and by electrophoresis. This was done in urines from 9 patients and a control group of 10 persons who had no contact with toxic substances. The contribution of particular isoenzymes in total activity was determined by the "batch-wise" method with DEAE-52 cellulose. Significant differences were discovered in thermostability of NAG isoenzymes which may be utilized in nephrological diagnosis.

Acetylglucosaminidase↗
PubMed1989

The effect of epsilon-aminocaproic acid (EACA) on experimental immune nephritis.

The EACA, known for its antihemorrhagic potential, has also been demonstrated to mitigate physiopathologic reactions linked activation of serum proteolytic cascades. Herein, we present results of experiments exploring a possible modification of nephrotoxic serum nephritis (NSN) in rats by the EACA. A combined intraperitoneal and oral administration of EACA to Lewis rats with the NSN (1 g/kg/12 h and 1 g/kg/24 h, respectively), significantly reduced albuminuria (p less than 0.05 EACA-treated rats vs. rats obtaining vehicle alone, days 2-3), enhanced endogenous creatinine clearance (ECC) - (p less than 0.01 - day 3) and attenuated renal histopathologic changes (day 3 post induction). The EACA given to control healthy rats did not cause any notable changes in the above parameters. We conclude that the EACA considerably diminishes the intensity of acute nephritis induced in rats by a nephrotoxic heteroantiserum.

Albuminuria↗