PubMed Health⌕ Search

Biomedical subjects

J Ku

Publications and source records attributed to J Ku.

23 records · Page 2Linked to original sources
PubMed1985

Calcium antagonists stimulate sperm motility in ejaculated human semen.

Three calcium antagonists, diltiazem, flunarizine and verapamil stimulated human sperm motility in vitro. Among them, diltiazem induced the largest amplitude of motility increase. The concentration-response curve of diltiazem was similar in shape to those of calcium chelators. EGTA, a calcium chelator, potentiated the stimulatory effect of diltiazem while A23187, a calcium ionophore, antagonized it. These observations supported our previous hypothesis that an increased concentration of calcium ion was detrimental to human sperm motility. Whether calcium antagonists could be used in the treatment of subfertile patients awaits further investigation.

Calcimycin↗
PubMed1985

Screening the in vitro sperm-immobilizing effect of some anticancer drugs.

The in vitro sperm-immobilizing effect of seven anticancer agents, namely cyclophosphamide, 5-fluorouracil, cytarabine, mitomycin-C, 6-mercaptopurine, doxorubicin and vinblastine were screened with a transmembrane migration method. Only doxorubicin and vinblastine inhibited human sperm motility. Because colchicine, a microtubular inhibitor, had no sperm-immobilizing effect, we suggest that the sperm membrane is the site of action of these two anticancer drugs to inhibit human sperm motility.

Antineoplastic Agents↗
PubMed1984

Glutathione and caffeine antagonize the sperm-immobilizing effect of a vaginal contraceptive.

The concentration-dependent inhibition of Neosampoon, a vaginal contraceptive, on ejaculated human sperm motility was studied with a transmembrane migration method. Caffeine (5mM), glutathione (5mM) and glutathione (15mM) all reactivated the motility of Neosampoon-immobilized sperm. Caffeine (5mM) was more potent than glutathione in antagonizing Neosampoon; there was no difference between the potency of 5mM- and 15mM-glutathione. Human sperm motility could be a model for studying the interaction of membrane-active drugs, including the protective effect of glutathione against membrane-disrupting agents.

Caffeine↗
PubMed1984

Sperm immobilizing potency of amitriptyline and imipramine: measured with transmembrane migration.

The inhibitory effect of amitriptyline and imipramine on human sperm motility was measured in vitro with a transmembrane migration method. The EC50 for amitriptyline and imipramine were 170 microM and 160 microM respectively. They were more potent than any other drug that had been tested on this pharmacological model. Transmembrane migration method was less sensitive than turbidimetric method to detect the sperm immobilizing effect of drugs.

Amitriptyline↗