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Biomedical subjects

J Kumate-Rodríguez

Publications and source records attributed to J Kumate-Rodríguez.

15 recordsLinked to original sources

Costs of treating diarrhoea in a children's hospital in Mexico City.

The treatment received by children aged under 5 years with diarrhoea was studied in the Hospital Infantil de México (Federico Goméz), Mexico City. The costs of treatment were calculated and estimates were made of how these had changed since the establishment of an oral rehydration unit in the hospital in 1985. The results indicate that drug treatment of outpatients was generally appropriate and inexpensive. In contrast, the cost of drugs for inpatients was considerably higher. The seriousness of the cases justified much of this additional expense for inpatients, but there is evidence that the costs could be reduced further without jeopardizing the quality of the care. Diagnostic tests were relatively expensive, frequently failed to identify diarrhoeal etiology, and their results correlated poorly with the treatment prescribed. The oral rehydration unit resulted in significant savings by causing a 25% fall in the number of inpatients with diarrhoea.

Antidiarrheals↗

[Identification of Vibrio cholerae O1 by flow cytometry].

A total of 72 peptonated water samples suspected of carrying Vibrio cholerae were assessed by laser flow cytometry (LFC) and compared with positive culture. We used a direct fluorescence technique using polyclonal (PolAb) and monoclonal antibodies (MoAb) conjugated to fluorescein. The PolAb were able to detect 33 positive samples. A clear difference among the 20 positive samples was found with only three V. cholerae O1 false negatives when MoAb were used whereas all 13 V. cholerae Non O1 samples were detected. The correlation index comparing control autofluorescence with peptonated water samples show a R = 0.69, versus 0.96 with pure V. cholerae O1 strains. Our data suggest that the LFC technique is able to recognize V. cholerae O1 from a mixture of microorganisms with high sensitivity and specificity in a few hours.

Antibodies, Bacterial↗

[The immunological phenotyping of the lymphocytes in HIV+ patients].

We propose that the ratios of lymphocyte subsets: CD4+/CD8+, CD4+/CD3+, and CD8+/CD3+, graphically displayed in this order, could be an easy way of immunophenotyping and a simple form of interpretation both for clinicians and patients. A total of 187 asymptomatic HIV-positive patients, -including 83 symptomatic patients with AIDS- and a normal comparative age group of 36 patients, were studied by flow cytometry. Ratios were graphically displayed on millimetric-grid paper, and a specific point was given to each obtained ratio. The points were connected by a line. The angles formed by the lines were measured and graphically displayed over a circle. The point of reference 0 degree was located on the left side of the circle, giving the corresponding 90 degrees, 180 degrees, and 240 degrees angles. The graph of the control group shows a "beach chair" image. As soon as the CD4+ lymphocytes diminish and the CD8+ lymphocytes increase in HIV+ patients, "the chair's headrest" descends and the "chair's footpiece" swivels back. Given the ranks and fluctuations of the lymphocyte subsets, their graphic display provides a better representation of the immunological status.

Adult↗

Assessment of viability of yeast by flow cytometry using Light Green as vital dye exclusion.

A comparative study of two different staining methods for estimation of yeast's viability is assessed by microbial flow cytometry. Propidium iodide (PI) a well known nucleic-acid binding vital dye exclusion is compared with light green (LG), a cytoplasmic function related vital dye widely used in histological, histochemical and immunocytochemical staining. The yeast model used was heat-killed Candida guilliermondii cells. The stained heat-killed bacterial population showed on the dot-plot flow cytometer analysis, a clear separation towards the green-orange (350-600 nm) for LG, and orange-red (550-725 nm) for PI on the fluorescence spectrum-zone channels. Both dyes showed by non-parametric Kolmcgorov-Smirnov, 99% of probability of difference between heat-killed and live microorganism's histogram-population, with a D = ranking from 0.50-0.64 for LG to 0.91-0.98 for PI. Higher cost and carcinogenic risk when handling PI, make LG a better choice for flow cytometry yeast viability tests.

Candida↗

[Bicentennial of smallpox vaccine: experiences and lessons].

A brief description of the effects that smallpox caused in the worldwide population places the events that gave rise to the evolution of the concept and practice of the smallpox vaccine in context. Through a description of various events in Jenner's life in relation to his study and use of inoculation, the lessons that have been learned from the dissemination of the technique and the development of smallpox vaccination campaigns and programs throughout the world are commented on. The article indicates that the analysis of the process that culminated in the eradication of smallpox has current applications in programs underway to control, eliminate and eventually eradicate certain diseases. Finally, some lessons are highlighted that have been used in successful national, regional and worldwide vaccination campaigns in the struggle against other infectious diseases.

History, 18th Century↗

[Measles vaccination by the aerosol method in Mexico].

The present work describes the anti-measles vaccination program by the inhaled aerosol method undertaken in Mexico between 1988 and 1990. Detailed descriptions are given of the equipment, staff, training programs, promotion and campaigns. The vaccine is specified: Edmonston-Zagreb strain cultured in diploid cells at the Instituto Nacional de Virología of the Secretaría de Salud in Mexico with titres varying from 1045 plaque forming units (PFU/ml) to 1048 PFU/ml administered in a 30 sec inhalation with aerosol. During this exposure period, 2800 to 4000 PFU per child are estimated to enter the child, of which approximately 25% is the retained doses, i.e. 700 to 1000 PFU/child. A total of 3760684 children of school and pre-school age have been vaccinated in 13 of the 32 federal entities of the country. No undesirable effects of any importance were observed, and the limited serological and field studies support the effectiveness and security of this method. On the other hand, the method is much cheaper, faster and better accepted by the population than the subcutaneous injection.

Aerosols↗