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J Kummert

Publications and source records attributed to J Kummert.

10 recordsLinked to original sources
PubMed1978

Reverse transcription of turnip yellow mosaic virus RNA primed with calf-thymus DNA hydrolysate: characterization of the purified cDNA product.

Complementary DNA was transcribed from turnip yellow mosaic virus RNA, using the method of Taylor et al. (1). The purified cDNA thus obtained sedimented between 2 and 4 S and was a mostly uniform transcript of template RNA. It hybridized with a sharp transition to homologous TYMV-RNA (Crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the RNAs of two other tymoviruses, namely Andean potato latent virus and eggplant mosaic virus.

Avian Myeloblastosis Virus↗
PubMed1976

Evaluation of the techniques used for investigating the in vitro synthesis of bromegrass mosaic virus RNA.

The paper is dealing with the study of RNA polymerase activity using endogenous RNA templates in extracts of leaves infected with bromegrass mosaic virus (BMV). The in vitro labeling of BMV-RNA in such extracts is associated with a particulate replication complex which is able to synthesize totally or partially single-stranded BMV-RNA under conditions where exogenous BMV-RNA (purified from the virus) is rapidly degraded. This suggests that rapidly-labeled BMV-RNA is protected from endogenous nuclease effect.

DNA-Directed RNA Polymerases↗