The sequence of a third member of the heat shock protein family in Plasmodium falciparum.
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Amino Acid Sequence↗
Biomedical subjects
J Kun
Publications and source records attributed to J Kun.
A versatile phage lambda expression vector system for cloning in Escherichia coli.
By integrating fragments from the expression plasmids pJK2 and pJK4 into a derivative of the bacteriophage lambda, we constructed the phage expression vectors lambda JK2 and lambda JK4, which allow efficient cloning of genomic or cDNA either into the 5' end or the 3' end of the lacZ gene of Escherichia coli. Expression of barrier-free DNA in phase may lead to fusion proteins consisting of active beta-galactosidase (beta Gal) plus an additional polypeptide encoded by the inserted DNA. Analysis of distinct recombinant clones is quick and easy, due to the reversible integration of the plasmid into the genome. As an example, we constructed an expression library of genomic Plasmodium falciparum DNA in lambda JK2. We polymerised (amplified) and expressed a synthetic DNA fragment, which codes for a potential antigenic determinant of the 11-1 gene of Plasmodium falciparum as a fusion to the N terminus of active beta Gal. We demonstrate that such chimeric molecules can be affinity-purified and that polypeptides can be separated from the beta Gal part by cleavage with the protease factor Xa.
Amino Acid Sequence↗
Cloning and expression of genomic DNA sequences coding for putative erythrocyte membrane-associated antigens of Plasmodium falciparum.
Genomic DNA fragments of Plasmodium falciparum generated by mung bean nuclease digestion were cloned in the lambda expression vector lambda JK2. The resulting library was screened with a rabbit antiserum raised against purified membranes of P. falciparum-infected erythrocytes and with a serum pool from immune humans from an endemic area of Liberia. Positive clones were rescreened with a series of human and monkey sera. Twelve selected clones were analysed in detail. Four of them corresponded to already described membrane-associated P. falciparum antigens. The other positive clones contained inserts which, according to the nucleotide sequence, Southern blot analysis and immunological characteristics, correspond to so far unknown antigens.
Amino Acid Sequence↗
[Usefulness of the fluorescein dilaurate test in patients after acute pancreatitis].
30 patients, 1 to 6 years acute pancreatitis, complaining on epigastric pains, weight loss, flatulence and diarrhea were examined. Temmler Werke Pancreolauryl-Test was performed following manufacturer's instructions. In 14 patients (46.7%), the result of the test revealed pancreatic exocrine failure. Those results were verified and confirmed in 80% by secretin-ceorulein test. Non-invasive, technically uncomplicated and reliable pancreolauryl-test seems to be useful for screen estimation of pancreatic exocrine function impairment in patients after the acute pancreatitis.
Acute Disease↗