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J Kurlenda

Publications and source records attributed to J Kurlenda.

6 recordsLinked to original sources
PubMed1998

[Hemolytic activity of Klebsiella pneumoniae and Klebsiella oxytoca].

We demonstrated that Klebsiella pneumoniae and Klebsiella oxytoca possess a selective haemolytic activity on rabbit erythrocytes. Thirty one Klebsiella strains (18 strains of K. pneumoniae and 13 strains of K. oxytoca) were isolated from hospitalized patients. The liquid (Trypcase-soy broth--TSB) and solid (Trypcase-soy agar--TSA) medium, containing the red cells were used for the tests. All the screened strains showed a haemolytic effect on rabbit erythrocytes, provided that the supernatants of the cultures were preincubated with beta-mercaptoethanol or calcium chloride. There was no human and sheep erythrocyte lysis.

Animals↗
PubMed1993

[Usefulness of plasmids as epidemiologic markers for typing of Klebsiella].

The study was aimed at evaluation of utility of plasmids as epidemiological markers for interspecific differentiation of 200 strains of Klebsiella isolated from patients with generalized infections and hospitalized at intensive care ward of the Gdańsk Medical Academy. Strains isolated from the ward environment were also investigated. In the first phase of this study classical methods of typing were used, such as determination of the biochemical, bacteriocin (susceptibility to 8 bacteriocins) and phage types, and MIC for 9 antibiotics. Basing on these methods it was found that 41 types of Klebsiella pneumoniae, 13 types of Klebsiella oxytoca and 1 type of Klebsiella ozenae occur. Six groups of bacteria were differentiated in the group which had the same type. These strains were investigated for plasmid and restrictive patterns. Identity of types occurring in individual groups was detected. For one of them, a restrictive pattern was determined by cleavage of plasmid DNA by a restrictive Eco RV enzyme. In remaining groups such pattern was not found, both after treatment with Eco RV and Hha I enzyme and this may be associated with modification of the plasmid DNA. Strains belonging to one of these groups did not contain plasmids in their cells.

Biomarkers↗
PubMed1990

[Mycoplasma pneumonia].

Two cases of mycoplasma pneumonias are presented. Initially this etiology was not suspected. Prior to hospitalization they were treated with cefamandole and carbenicillin and cephradine with gentamycin without any improvement in the clinical state of the patients. After serological tests finding a high titer of serum antibodies (1: 2560) the therapy was altered in the first patient to minocycline (in a dose of 200 mg per day for 16 days) and in the second to doxycycline (in a dose of 200 mg per day for 10 days). The first patient improved clinically after two days of altered therapy, the second after 10 days. Both patients were discharged fully recovered after 32 and 36 days of hospitalization.

Adult↗
PubMed1989

Phage typing of Klebsiella strains in epidemiological studies.

A set of 14 routinely applied bacteriophages was used for the typing of Klebsiella pneumoniae and Klebsiella oxytoca strains isolated from patients in 1983-1986. The phage-sensitive strains (65%) differed in respect to the place and time of isolation which manifested itself by an occurrence of specific lysotypes in Wrocław, Gdańsk, Wernigerode and Włocławek, as well as various repeatibility on the lysotypes within 10 years.

Bacterial Typing Techniques↗