A comparative study of the isoenzymes of mammalian alpha-amylase.
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Biomedical subjects
Publications and source records attributed to J L Bell.
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THE GLYCERIDE GLYCEROL ANALYSIS DEPENDS, AFTER SAPONIFICATION OF TRIGLYCERIDES, ON A LINKED ENZYMATIC PROCEDURE USING GLYCEROKINASE, PYRUVATE KINASE, AND LACTATE DEHYDROGENASE: the final conversion of NADH to NAD(+) is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104%. In a mixed male and female group the normal range for glyceride glycerol was 2.5 to 15.5 mg/100 ml (0.2-1.4 mmol/l) fasting, and 2.5 to 18.0 mg/100 ml (0.2-1.6 mmol/l) postprandially using fresh serum. There was a significant rise postprandially in older men.
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The reliability of five commercially produced immunological pregnancy diagnosis methods has been investigated. The tests used were Pregnosticon (a tube test) and four slide tests, Hyland, Pregslide, Gravindex, and Planotest. In the series described, Planotest gave 0.5% false positives, Gravindex had 2.1%, Pregnosticon 2.6%, and Pregslide 4.7%, Hyland A (sensitivity 4,500 iu/l.) gave 3.6% and Hyland B (sensitivity 2 to 3,000 iu/l.) had 8.7% false positives. Pregnosticon had 0.5% false negatives, Planotest 2.0%, Gravindex 3.5%, Hyland B 6.5%, Pregslide 9%, and Hyland A 19.5% false negatives. Planotest and Pregnosticon were found to be less influenced by protein and blood in the urine than the other pregnancy tests investigated.
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