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Biomedical subjects

J L Braidwood

Publications and source records attributed to J L Braidwood.

11 recordsLinked to original sources

Laboratory testing closer to the patient: the Canadian scene.

Laboratory testing closer to the patient in Canada takes several forms. Driving forces include the expectation that this technology will result in improved patient care. Geographic remoteness of large parts of the country is another factor, as is the critical shortage of certain clinical specialists such as neonatologists. Increasingly, the medical, scientific and technical professions are guiding--if not always controlling--the process. The Canadian Council on Health Facility Accreditation is developing new guidelines which will assist in ensuring that laboratory personnel are intimately involved in the organisation and control of bedside monitoring. The Federal Bureau of Radiation and Medical Devices, as well as hospital-based professionals, have been very active in the assessment of devices used for this emerging technology.

Blood Glucose

Comparison of column chromatographic, colorimetric and electrophoretic methods for determination of glycosylated hemoglobin (HbA1).

A comparison of two column chromatographic (BioRad & Helena), an electrophoretic (Corning) and a colorimetric method for the determination of glycosylated hemoglobin (HbA1) was performed. Blood samples were obtained from a diverse diabetic population and normal persons. Inter-and intra-run precision studies with normal and above normal HbA1 samples indicated that there was no statistically significant difference (p greater than 0.05) in precision among the methods. The electrophoretic and BioRad column chromatographic methods showed best correlation (r = 0.96 p less than 0.001 y = 0.465 + 1.036 x), while the colorimetric method gave HbA1 levels consistently higher than those obtained by electrophoresis (r = 0.852 p less than 0.001 y = 4.99 + 0.867 x). The effects of preincubation to remove labile glycosylated hemoglobin with saline or semicarbazide - aniline preincubation were also studied. There was no statistically significant difference (p greater than 0.05) in the HbA1 levels from erythrocytes after saline preincubation as compared to nontreated samples. Further investigation concerning the value of preincubation to remove the labile HbA1 fraction is warranted.

Chromatography, Ion Exchange