PubMed Health⌕ Search

Biomedical subjects

J L Ericsson

Publications and source records attributed to J L Ericsson.

15 recordsLinked to original sources
PubMed1979

Giant cell tumor of bone. Variations in patterns of appearance of different cell types.

Eleven benign giant cell tumors of bone were studied in the electron microscope, and the fine structural localization of acid phosphatase was elucidated. Three distinct cell types are always present in these tumors: stromal cells type 1; stromal cells type 2; and multinucleated giant cells. Small mononuclear cells may also occur, but are not likely to be actively participating in the neoplastic process. The range of variability in the fine structure of the different cell types constituting this tumor has been established. Variations in appearances include: a) presence of nuclear pseudoinclusions in stromal cells type 1 and multinucleated giant cells; b) aberrations in the structure of the rough surfaced endoplasmic reticulum in the same cell types; c) occurrence of ruffled borders, ectoplasmic layers and cytoplasmic labyrinths containing acid phosphatase in the giant cells. Some giant cells show evidence of marked phagocytic activity and contain large and numerous residual bodies carrying acid phosphatase. The significance of the interrelations between the different cell types are discussed and the possible role of stromal cells type 2 in immunological mechanisms directed against the tumor cells are mentioned.

Acid Phosphatase↗
PubMed1979

Ultrastructural features of cultured human glia and glioma cells.

The fine structure of three lines of human normal glial cells and eight established lines of malignant glioma cells are described. The glial cell lines were ultrastructurally very similar whereas the glioma cell lines differed greatly from one another. In sparse proliferating cultures there were no consistent findings which distinguished the glioma cell lines as a group from the normal glial cells. Only in post-confluent cultures could the consistently irregular cell surfaces and ruffling, both at the cell periphery and centrally on the upper cell surface, with associated pinocytosis, distinguish the glioma from the post-confluent glial cultures, which did not possess these properties. The common attributes of post-confluent glioma cells reflect the cells' continued proliferation. The glioma lines did display individual ultrastructural characteristics which appear to be stable, the glioma lines having retained these during a number of years of continual passage.

Cell Division↗
PubMed1979

Studies on the rat liver following iron overload. Electron probe x-ray microanalysis of acid phosphatase and iron.

Previous electron-microscopic studies on the liver have shown that following excessive administration of iron to experimental animals, small particles believed to represent ferritin and/or hemosiderin (electron-dense iron-containing particles [IPs]) accumulate in membrane-bound bodies--many with a lysosome-like structure--in liver parenchymal and Kupffer cells. Further identification of the IP-containing bodies has been facilitated by the application of histochemical techniques for the demonstration of acid phosphatase. The results have shown that reaction product was deposited over organelles similar in appearance to the IP-containing ones, indicating that they were lysosomes. However, the granular nature of the reaction product makes it difficult or impossible to decide whether IPs are present simultaneously with reaction product in the organelle. In order to clarify this qualitative aspect, x-ray microanalysis has been utilized to identify iron and lead (reaction product) in the various structures thought to represent lysosomes. The results indicate that all IP-containing bodies also show the presence of reaction product, and thus can be regarded as lysosomes. However, in the parenchymal cells there may exist a small population of iron-deficient lysosomes (only lead could be shown). The latter may correspond to "primary lysosomes."

Acid Phosphatase↗
PubMed1979

Ultrastructure of the aorta in experimental uraemia.

The effect of chronic experimental uraemia on the ultrastructure of the aorta was studied in rats. Pathological changes were found essentially in the media, in which the smooth muscle cells commonly had increased amounts of endoplasmic reticulum, Golgi structures and mitochondria, probably reflecting an increased synthetic and metabolic activity. In other areas of the media, degenerative and necrotic changes of the smooth muscle cells dominated. Increased amountes of collagen and the appearance of vesicular structures, probably cell debris, were noted in the extracellular space. Diffusely spread needle- and plate-shaped electron dense structures were seen in some necrotic areas. By X-ray microanalysis these structures could be demonstrated to have a high content of calcium, and probably represented hydroxyapatite crystals. Two types of circumscribed rounded electron dense conglomerates also appeared. At least one of these structures, in which X-ray microanalysis showed a high calcium content, is thought to represent a site of early calcification. Electron dense deposits in the elastic structures were only occasionally seen. The alterations occurred in both parathyroidectomized and non-parathyroidectomized uraemic animals. The study revealed no signs of intracellular calcification and suggests a close relation between necrosis of the smooth muscle cells and calcification of the aortic wall in experimental uraemia.

Animals↗
PubMed1979

Arterial lesions of the radial artery in uraemic patients.

Arterial specimens from the radial artery were removed in connection with Brescia fistula operations on 15 uraemic patients and were studied by light and electron microscopy. The patients lacked clinical signs of arterial insufficiency. Radial artery specimens from 15 humans that underwent forensic post-mortem examination served as controls. The intima of the uraemic patients was significantly thicker than that of the controls. In 8 of the uraemic arteries light microscopy disclosed necrotic areas in the media and in 2 cases such areas in the intima, compared to only one case with patchy medial necrosis in the controls. Calcification was histochemically demonstrated in 6 of the uraemic arteries while none of the controls showed this change. The presence of degenerated and necrotic smooth-muscle cells was verified at the ultrastructural level. Furthermore, modified smooth-muscle cells appeared in the intima and the media and were probably of significance for the synthesis of the increased amounts of collagen and mucopolysaccharides that could be demonstrated. Ultrastructurally evidence of calcification could be demonstrated in the internal elastic membrane and in necrotic areas in the intima and the media. In the latter localization the calcification process seemed to start in relation to extracellular vesicular structures, probably representing cell debris. The possible significance of the observed changes for the development of symptom-giving arterial disease is discussed.

Adult↗
PubMed1978

Malignant giant cell tumor of bone. Fine structure and localization of acid phosphatase.

The fine structure of the different cell types constituting a primary malignant giant cell tumor of bone has been studied and the localization of acid phosphatase in relation to the subcellular organelles been demonstrated. Three distinct cell types with characteristic ultrastructural features were observed: giant cells, fibroblast-like cells, and cells with abundant lipid inclusions and mitochondria. Certain differences were noted between these three cell types and their counterparts in benign giant cell tumors of bone (described in a separate report). The enzyme histochemical and morphological data suggested that the giant cells in the malignant tumor might possess a more active and expansive lysosomal apparatus than corresponding cells in the benign variant.

Acid Phosphatase↗
PubMed1978

Giant cell tumor of bone. Fine structural localization of alkaline phosphatase.

The fine structural localization of nonspecific alkaline phosphatase was elucidated in two giant cell tumors of bone using lead as capturing ion and beta-glycerophosphate as substrate in the incubation solution. Lead phosphate precipitate--indicating presence of alkaline phosphatase--was demonstrated on the plasma membranes, and the membranes bordering vesicles and vacuoles of presumed endocytotic nature, in giant cells and type 1 stromal cells (fibroblast-like cells). The findings support the view that stromal cells type I and giant cells are histogenetically related.

Adult↗
PubMed1978

Studies on the fine structure of osteoblastoma with notes on the localization of nonspecific acid and alkaline phosphatase.

Electron microscopy of two osteoblastomas revealed the existence of three distinct types of cells in this tumor: osteoblast like, macrophage like, and multinucleated giant cells. In addition to the lysosomes, most Golgi cisternae and vesicles in the osteoblast like cells showed evidence of acid phosphatase activity. Deposits of lead phosphate indicating the site of this enzyme in the macrophage like cells were confined to the large and abundant lysosomes. Wide spread deposition of final product was noted in the cytoplasm of the multinucleated giant cells, both in conventional lysosomes, Golgi regions and special organelles probably corresponding to GERL. With regard to nonspecific alkaline phosphatase, final product indicating the location of enzyme activity was confined to the plasma membranes and associated vesicular and vacuolar structures in the osteoblast like cells. The findings suggest that the giant cells in osteoblastomas participate in lytic bone destructive and resorptive processes while osteoblast like cells appear to be osteoid and bone forming carriers of the neoplastic properties of the tumor.

Acid Phosphatase↗
PubMed1978

Childhood cancer in Sweden, 1958-1974. I. Incidence and mortality.

All cases of tumours and tumour-like conditions in children 0-14 years reported to the Swedish cancer registry during the period 1958-74 have been studied. The material consists of 3797 individuals on file in this registry. The most common cancer diagnoses in children 0-14 years are leukemia and tumours of the central nervous system (together constituting approximately 58% of all cases). Almost half of the cancers affect children below five years of age. The lowest incidence occurs in the ages 7-8 years, and the highest occurs during the first year of life. The types of tumours below one year of age show a different distribution than in any other age groups. A significant increase in the incidence of childhood cancer occurred, while the mortality rates showed a slight decrease during the period studied. A remarkable increase in the incidence figures was noted concerning tumours of the nervous system, especially in boys. The decrease in the mortality rates was most obvious regarding Wilms' tumour, and leukemia in children 0-4 years of age.

Adolescent↗
PubMed1978

Contribution to the knowledge of the fine structure of chondrosarcoma of bone. With a note on the localization of alkaline phosphatase and "ATPase".

Seven well differentiated chondrosarcomas of bone have been analyzed by electron microscopy, and the fine structural localization of adenosine triphosphatase and nonspecific alkaline phosphatase has been elucidated. On the basis of the fine structural appearance, two distinct cell types were shown to constitute the tumor tissue: chondrocyte-like cells and large "mitochondria-rich cells". Large, multinucleated cells in the tumor did not seem to correspond to osteoclasts but rather were likely to represent true neoplastic cells. Some chondrocyte-like cells appeared to be binucleated by virtue of deep, groove-like nuclear indentations. Adenosine triphosphatase and alkaline phosphatase were associated with the plasma membrane of both chondrocyte-like and mitochondria-rich cells suggesting that they might be of common origin. Normal chondroblasts and chondrocytes lack histochemically demonstrable adenosine triphosphatase on their plasma membrane. Presence of this enzyme in the tumor cells may indicate that they are histogenetically related to immature non-chondroid matrix forming cells (known to carry the enzymes).

Adenosine Triphosphatases↗
PubMed1978

Cytoplasmic effects of X-irradiation on cultured cells in a nondividing stage. 3. Alterations in plasma membrane motility.

Cultured, density-dependent growth inhibited human glia cells were exposed to X-radiation, generated by an 8-MeV linear accelerator, at a dose of 200 Gy. Phase contrast microscopy, time-lapse cinemicrography and scanning electron microscopy showed the irradiated cells to have increased ruffling activity of plasma membranes and enhanced macropinocytosis with a maximum approximately 24 hours after irradiation. "Atypical" central ruffles arising from the upper cell surface were demonstrated on some irradiated cells. The turnover of plasma membranes was supposed to be increased in the irradiated cells resulting in the formation of the observed branched, thread-like cells. The diminished cell surface area was believed to result from an unbalance between degradation and renewal of the plasma membranes in the irradiated cells.

Cell Line↗
PubMed1977

Giant cell tumor of bone: fine structural localization of acid phosphatase.

The fine structural localization of acid phosphatase in the different cells in a benign giant cell tumor of bone has been studied. Stromal cells type 1 and 2 (fibroblast-like and macrophage-like, respectively) showed the presence of lead phosphate precipitate following incubation in a Gomori-type lead salt medium only in conventional lysosomes. In the multinucleated giant cells, the final product was deposited over lysosome-like organelles, and also over Golgi cisternae, vesicles, and vacuoles. Furthermore, evidence for presence of acid phosphatase was obtained in smooth-surfaced tubular, sausage-, horse-shoe-, and ring-shaped structures and over digestive vacuoles of autophagic or heterophagic origin. Finally, in these cells, many of the tubular and vacuolar elements located subjacent to areas of the plasma membrane with microvillous specializations (abortive brush borders?) were shown to carry acid phosphatase.

Acid Phosphatase↗