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Biomedical subjects

J L Gadberry

Publications and source records attributed to J L Gadberry.

7 recordsLinked to original sources

Branhamella catarrhalis as a cause of pneumonia in a patient with miliary tuberculosis.

Branhamella catarrhalis is increasingly reported as a cause of pneumonia in the immunocompromised host. The authors here report what they believe to be a unique case of B catarrhalis bronchopneumonia in a patient who had previously acquired miliary tuberculosis. The patient initially responded to medication but died suddenly following a brief episode of febrile illness. At autopsy, several lines of evidence implicated B catarrhalis in the findings. The authors review the literature regarding cases of lower respiratory tract infection reportedly caused by B catarrhalis. Their own conclusion is that B catarrhalis infection is not necessarily caused by abnormal immunoglobulin, as some workers have suggested, but rather by damaged lung tissue in general and damaged bronchoalveolar cells in particular.

Aged

Comparison of a new commercially prepared porphyrin test and the conventional satellite test for the identification of Haemophilus species that require the X factor.

A test with a commercially developed porphyrin test agar was examined for the identification of Haemophilus spp. The porphyrin test agar method was compared with the conventional paper strip satellite method in tests with 187 isolates and was found to be easier to perform and interpret, giving a sensitivity of 98.7% and specificity of 94.7%.

Agar

Increased indole detection for Pasteurella multocida.

A supplemented 2% peptone broth is described for the detection of indole production by Pasteurella multocida. The 96 isolates of P. multocida that were utilized in this evaluation were indole positive within 18 to 24 h.

Bacteriological Techniques

Evaluation of methods to detect oxidase activity in the genus Pasteurella.

Several oxidase reagents and commercial products were evaluated as to their efficacy in detecting oxidase activity in species of the genus Pasteurella. Recommendations are made concerning the reagent of choice for determining oxidase activity in the genus Pasteurella. Recommendations are made also concerning the use of commercial products and their efficacy in detecting oxidase activity in this genus.

Animals

Characterization of a Pasteurella multocida bacteriophage.

The Pasteurella multocida bacteriophage B939a produces turbid plaques, which suggests infection with a temperate phage. Attempts to induce production of lytic phage by UV irradiation were unsuccessful, however, Bateriophage B932a is highly stable at 4 C and relatively stable at room temperature. It tolerates moist heat at 60 C, but is inactivated at 70 C in 10 minutes. Morphologically, it is a tailed phage with a hexagonal head.

Bacteriophages