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Biomedical subjects

J L Jacob

Publications and source records attributed to J L Jacob.

At least 55 records · Page 3Linked to original sources
PubMed1990

Detection by electron microscopy of a small subband 13q14.11 deletion in an hereditary retinoblastoma.

High-resolution banding, specific for electron microscopy, was applied to chromosomes of synchronized blood lymphocytes obtained from a child with bilateral retinoblastoma. Ultrastructural analysis of the subbands in region q14.1, after synchronization and immunochemical banding, showed that the deletion in the abnormal chromosome 13 corresponds to subband 14.11, thus evidencing that the retinoblastoma gene is located within subband q14.11. This first application to a diagnostic problem of immunochemical banding suggests that, coupled with electron microscopy, this banding provides a higher resolution than that obtained with light microscopy and should be useful to pinpoint important localizations.

Child, Preschool↗
PubMed1990

[Traumatic ventricular septal defect. A case report].

A 34 years old woman, without previous cardiac symptoms, suffered a closed thoracic trauma in a car accident. Three days after presented dyspnea and orthopnea. Physical examination revealed a systolic murmur consistent with ventricular septal defect (VSD). Doppler echocardiography and angiography confirmed the presence of a muscular VSD with severe left-to-right shunt. Surgical intervention was indicated and VSD closure was performed with a patch, through a right atrium approach. Third degree atrioventricular block developed after surgery and a definitive pacemaker was implanted. The patient is asymptomatic and without murmurs in postoperative follow-up.

Accidents, Traffic↗
PubMed1989

Clinical and laboratory signs of reperfusion: are they reliable?

We studied 101 patients (88 men and 13 women, mean age 54.5 +/- 10 years) who arrived at the hospital during the first 6 hours of acute myocardial infarction evolution. Our objective was to assess the reliability of clinical and laboratory signs of recanalization using intravenous streptokinase as a thrombolytic agent. The mean time between the beginning of infusion and coronary arteriography was 53.83 +/- 43 hours. The positive predictive values for pain, arrhythmia, ST segment and enzymes were 97.9%, 94.2%, 91.8% and 90.8%, respectively; the negative predictive values were 46.8%, 40.8%, 37.2%, and 50% in the same order. Sensitivity was 65.7%, 62.8%, 58.4% and 77.6% and specificity 95.6%, 86.9%, 82.6% and 73.9%, respectively. The positive predictive value, calculated on the basis of the presence of each variable alone or in association showed a probability of recanalization of 76.9% for one sign, 84% for two, 96.3% for three and 100% for all four. When we compared the positive predictive values of each variable according to the interval between the beginning of pain and admission to the hospital (during the first 3 hours or between 3 and 6 hours) our results were 100%/94% for pain (P = NS), 97%/88% for arrhythmia (P = NS), 100%/75% for ST segment (P = 0.004), and 97%/80% for enzymes (P = 0.019). The same analysis applied to negative predictive values showing 22%/62% (P = 0.007), 17%/55% (P = 0.008), 21%/47% (P = NS), 27%/61% (P = NS) for pain, arrhythmia, ST segment and enzymes, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[Myocarditis simulating acute myocardial infarction. A case report].

A six month-old infant presenting congestive heart failure and electrocardiographic features of acute myocardial infarction, including increased CK-MB levels. The echocardiographic study showed normal origin of the coronary arteries and features compatible with myocardiopathy. After good early response to conventional therapy, the patient developed severe congestive heart failure and shock, and died. In the post-mortem study, the coronary arteries were previous. Microscopic study was compatible with acute myocarditis.

Creatine Kinase↗
PubMed1989

[Pulmonary artery aneurysm. Report of 2 cases].

The clinical suspicion of pulmonary artery aneurysm was made in two patients from the clinical signs and radiographic features. The echocardiographic studies showed features concerned with this pathology, and hemodynamic studies confirmed the diagnosis by pulmonary angiography. Both patients presented pulmonary valve insufficiency due to the huge pulmonary artery and dilatation of the valvular annulus. Other congenital malformations of the heart and pulmonary hypertension were not present in these patients. Surgical treatment was indicated in both patients. Surgery in patient no. 2 consisted of resection of the main pulmonary artery aneurysm and replacement with a valved conduit bovine pericardium and anastomosed with a patch of bovine pericardium connecting the left and right pulmonary arteries. The postoperative period was uneventful.

Aneurysm↗
PubMed1989

[Ebstein's anomaly of the mitral valve. A very unusual congenital malformation of the heart. A case report].

Ebstein anomaly of the tricuspid valve is a well known malformation of the heart, but this occurrence in the mitral valve is a very rare congenital heart disease. In this case report a two years and nine months old girl with symptoms of heart failure during the first year of life due to severe mitral regurgitation was studied. The echocardiographic and angiographic features were typical of the Ebstein malformation of the mitral valve. The surgical treatment was made with implantation of pericardial bovine bioprothesis in the mitral position, without ressection of the natural valve tissue. The surgical features confirmed the diagnosis and the postoperative evolvement is excellent.

Bioprosthesis↗
PubMed1988

The oculorespiratory reflex revisited.

Continuous measurement of the intratracheal pressure and capnography are very simple and accessible methods for the detection and recording of the oculorespiratory reflex (ORR). Eight healthy children (five to 14 years old) undergoing strabismus surgery under halothane-nitrous oxide anaesthesia with spontaneous ventilation were studied. The ORR was evoked by traction on the extrinsic muscles of the eye (four medial recti and four lateral recti). Slowing of the respiratory rate and/or shallow respiratory movements were observed in each patient. One patient developed apnoea of 20 seconds duration which forced the use of manually controlled ventilation. Intravenous atropine (0.01 mg.kg-1) reduced the incidence of positive OCR (to 37.5 per cent) but did not diminish the incidence of the ORR (100 per cent). Since the ORR may lead to hypercapnia and hypoxaemia, controlled ventilation is recommended for patients undergoing strabismus surgery at least immediately before and during the muscular traction. It is now clear that the ORR may be evoked by traction on the extrinsic muscles of the eye, may produce hypercapnia and hypoxaemia and so, may aggravate the consequences of the OCR.

Adolescent↗
PubMed1988

Preschool vision testing with a new device, the Scolatest.

To determine the reliability of results obtained in preschool children by school nurses with a new device for testing vision, the Scolatest, 153 children aged 4 to 5 years were presented with the Snellen E on the standard American Optical projector and with five letters of the Sheridan test (H, O, V, T and X) on the Scolatest. Another 212 children aged 3 to 4 years were presented with the Allen symbols on the projector and with the Pigassou symbols on the Scolatest. The tests with the projector were given at 6 m by an experienced pediatric orthoptist, those with the Scolatest at 2.5 m by a school nurse. The proportion of good responses was higher with the Pigassou symbols than with the Allen symbols. The results with the Sheridan letters and the Snellen E were similar up to 20/30; beyond 20/30 the Snellen E appeared unreliable. The four vision-testing systems were also presented by a school nurse at 2.5 m on the Scolatest to 102 children aged 4 to 5 years (Snellen E and the five Sheridan letters) and 80 children aged 3 to 4 years (Allen and Pigassou symbols). The proportions of good responses were more comparable. The results suggest that a working distance of 2.5 m should be used in testing vision in preschool children. Visual screening at this distance should test acuity beyond the 20/30 equivalence if results comparable to those at 20 feet with the 20/30 standard requirement are to be obtained.

Child, Preschool↗
PubMed1987

[Demonstration of a pyrophosphate-fructose 6-phosphate 1-phosphotransferase in the latex from Hevea brasiliensis].

A pyrophosphate: fructose-6-phosphate 1-phosphotransferase activity (EC 2.7.1.90) has been characterized in cytosol from Hevea brasiliensis latex. It is Mg+ dependent enzyme, and the cation has an optimal effect between 2.5 to 3 mM for a concentration of 1 mM of pyrophosphate and 10 mM of fructose-6-phosphate. It is activated by catalytic content of fructose-2,6-diphosphate. Its potential activity is higher than 40% of that of ATP dependent phosphofructokinase (EC 2.7.1.11). Its optimum pH is between 7.5-7.6; then, the enzyme affinity is 0.3 mM for pyrophosphate and 3.5 mM for fructose-6-phosphate. It is suggested that the transferase plays a role in the pyrophosphate metabolism and the increasing of the energetic efficiency of glycolysis and so takes a significant part in the biochemical mechanisms involved in the latex yield.

Cytosol↗