Biomedical subjects

J L Lognonné

Publications and source records attributed to J L Lognonné.

2 recordsLinked to original sources

PubMed1994

2D-page analysis: a practical guide to principle critical parameters.

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is a technique that is becoming more and more popular as the secrets of the critical parameters involved in its execution are being continually unravelled. The majority of these parameters are linked to the successful realization of the first dimension. Since the description of the original technique by O'Farrell in 1975, numerous studies have contributed to the improvement of the performance of 2D-PAGE. In this article, solutions to the problems most frequently encountered by 2D-PAGE users are given, such as cathodic drift, focusing at alkaline pH, the solubilization and the loading of the sample and different electrical parameters.

Electrophoresis, Gel, Two-Dimensional↗

PubMed1994

Image analysis of 2D gels: considerations and insights.

2D gel experiments require computer based analysis when the number of gels exceed four and quantitative information is required. Computer based systems should first perform the required tasks and should do so with enough ease and logic that users in a biological laboratory can easily use it on an intermittent basis. Considerations for analysis systems including input device specifications needed, computer hardware configurations and software tools are discussed and compared to the application requirements. The components of this system are then examined as a whole to envision how the total solution should function optimally in an biological laboratory.

Computers↗