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Biomedical subjects

J L Wilson

Publications and source records attributed to J L Wilson.

At least 19 recordsLinked to original sources
PubMed1992

The Clinical Oncology Career Development Award of the American Cancer Society. A 5-year evaluation.

BACKGROUND: In 1985, the American Cancer Society (ACS) replaced their Junior Faculty Clinical Oncology Program with the 3-year Clinical Oncology Career Development Award (COCDA). It was proposed that the longer duration and larger stipend of the COCDA would have a greater impact in encouraging promising young clinical oncologists to enter and remain in academic oncology. The COCDA was evaluated at this time to determine whether these goals had been achieved. METHODS: A questionnaire was sent to 104 of the total of 121 people who received awards in 1985, 1986, and 1987. RESULTS: A review of the 85 completed questionnaires showed that the COCDA had a significant impact on the recipients. Most have remained in academic settings and have flourished, as evidenced by their publication record and success in obtaining extramural funds, despite their clinical orientation. CONCLUSIONS: The COCDA has achieved its initial goals and will remain an important part of the professional education efforts of the ACS.

Authorship↗
PubMed1992

Production of human papillomavirus and modulation of the infectious program in epithelial raft cultures. OFF.

Human papillomaviruses trophic for anogenital epithelia cause benign warts, and certain genotypes are closely associated with cervical neoplasia. By using our modifications of the epithelial raft culture system, we were able to recapitulate and modulate the infectious program of a papillomavirus in vitro for the first time. Small pieces of a condyloma containing human papillomavirus type 11 were explanted onto a dermal equivalent consisting of a collagen matrix with fibroblasts and were cultured at the medium-air interface. The infected stem cells proliferated rapidly across the matrix, stratified, and differentiated, as judged by histology. The results correlated with the state of epithelial differentiation, which, in turn, was dependent on the type of fibroblast in the matrix. Under conditions where the epithelial outgrowth underwent terminal differentiation, the entire productive program took place, leading to virion assembly. In contrast, using an alternative condition where the outgrowth failed to achieve terminal differentiation, only the E-region RNAs from the E1 promoter accumulated to any appreciable extent. The proliferating cell nuclear antigen was induced in the differentiated suprabasal cells in the productive cyst growth, which also exhibited high copy viral DNA and abundant E6-E7 RNAs. Comparable cells in the nonproductive cyst outgrowth were negative for all three. These results suggest that the E6 and E7 proteins may play a role in establishing a cellular environment conducive to vegetative viral replication. The culture conditions described should be useful for genetic analysis of this family of important human pathogens and for testing potential pharmacological agents.

3T3 Cells↗
PubMed1992

Relationship of hen age and egg sequence position with fertility, hatchability, viability, and preincubation embryonic development in broiler breeders.

Indian River broiler breeder hens (n = 29) were caged individually to investigate whether hen age and egg sequence position were related significantly to the dependent variables fertility, hatchability, viability (hatch of fertile eggs), and preincubation embryonic development. Hens were artificially inseminated once per week. All eggs laid during the period of 31 to 54 wk of age were stored at 16 to 17 C for .5 to 7 days. Time of oviposition records were used to assign eggs to sequence position ("first" or "subsequent"). Eggs laid on odd-numbered weeks were broken open, fertility determined, and embryonic development staged. Eggs laid on even-numbered weeks were sent to a commercial hatchery to assess hatchability. Unhatched eggs were opened to determine fertility and embryonic mortality. In addition, hen weight, number of days since insemination, time of oviposition, and egg weight were recorded to determine their relationship to the dependent variables. Fertility (n = 3,240 eggs) and hatchability (n = 1,653 eggs) were not significantly related to egg sequence position, but were related to hen age (P = .0001 and P = .0002, respectively). Older hens demonstrated lower fertility and hatchability. In contrast, embryo viability (n = 1,487 eggs) and preincubation embryonic development (n = 1,200 eggs) were not significantly related to hen age, but were related to egg sequence position (P = .0026 and P = .0001, respectively). First-of-sequence eggs had lower viability, and embryos of these eggs were more developed than embryos of subsequent eggs. These data indicate that the reduction in chick production observed as the hen ages may be due to the increased incidence of first-of-sequence eggs.

Aging↗
PubMed1992

Research note: variability in preincubation embryonic development in domestic fowl. 2. Effects of duration of egg storage period.

Embryos of eggs from Single Comb White Leghorn hens were analyzed to determine whether duration of egg storage significantly affects embryonic development prior to incubation. Eggs were gathered over a period of 5 days from 25-wk-old hens that were naturally inseminated and housed in floor pens. Within 1 h of oviposition the eggs were collected and assigned randomly to one of five storage treatment groups of 0, 4, 7, 14, or 21 days. Fresh egg weight was recorded and the eggs were placed on plastic egg flats and stored at 14 C. Weight of each egg was measured after storage to determine amount of weight lost during storage. A total of 500 embryos were examined after storage. Viable embryos were staged for development using a modified Eyal-Giladi and Kochav classification. The incidence of embryonic mortality was noted. Duration of storage (P = .5815) collection date (P = .5815), and fresh egg weight (P = .3789) did not affect embryonic development significantly. A significant linear relationship was observed between duration of the storage period and loss of egg weight (P = .0001). Embryonic mortality was significantly related to loss of egg weight (P = .0001). Mortality was highest in eggs that were stored for a longer period of time. The data from the current study indicate that storing fertile eggs below physiological zero inhibits embryonic development. One of the reasons for the increased incidence of embryonic mortality in eggs that were stored for longer periods may be related to the increased egg weight loss in these eggs.

Animals↗
PubMed1992

Epithelial-specific gene expression during differentiation of stratified primary human keratinocyte cultures.

Cultured epithelial cells are used to generate extensive patches of autologous skin equivalent for patients with burns or wounds and to investigate the growth and differentiation of epithelia in vitro. We have undertaken a comprehensive study of the morphological and molecular events that occur during culturing of human foreskin keratinocytes at the liquid-air interface on a dermal equivalent consisting of a collagen matrix containing fibroblasts. Using radioactively labeled RNA probes for mRNAs and monoclonal antibodies for proteins, we found that the expression of a comprehensive set of differentiation stage-specific genes was affected by the type of fibroblasts included in the matrix as well as by the age of the culture. The expression of these genes was not always coordinated and could not be predicted from the histological appearance of the stratified epithelium. Surprisingly, the mouse fibroblasts promoted epithelial differentiation much more closely resembling foreskin than did the homologous primary foreskin fibroblasts.

3T3 Cells↗
PubMed1991

Patient perceptions.

The cancer patient's perceptions about treatment, prognosis, and long-term care have emerged in the context of interaction with the changing healthcare system. A brief overview of cancer patients' perceptions regarding their disease and subsequent care is provided. The economic, organizational, and technological environment in which this care is provided and the patient's perceptions of that environment are discussed. In addition to economic pressures, the ever-increasing number of cancer patients, prolonged survival, and patients' perceptions have created changes in the healthcare system. These changes are mediated by important socioeconomic, cultural, and demographic characteristics of the cancer patient. Recommendations to address these changes are discussed.

Attitude to Health↗
PubMed1991

Course and distribution of efferent fibers in the cochlea of the mouse.

The course, distribution and termination of single efferent fibers to the cochlea has been described in only a few animals and relatively few fibers have been studied with knowledge of their ipsilateral or contralateral origin. In order to examine the efferent fibers in the mouse, the anterograde tracer Phaseolus vulgaris leucoagglutinin (PHA-L) was iontophoretically injected into one side of the brain stem near the location of known efferent nuclei. Examination of surface preparations of the cochlea revealed detailed information for both the lateral olivocochlear (LOC) and medial olivocochlear (MOC) systems. Many, but not all, fibers entered the cochlea within the intraganglionic spiral bundle (IGSB). The LOC fibers were restricted to the ipsilateral cochlea and rarely branched within the IGSB and osseous spiral lamina (OSL). In the organ of Corti, they traveled either basally or apically in the region of the inner hair cells (IHCs), spanning lengths up to 130 microns (basally) and 890 microns (apically). Terminal swellings of these fibers were ca 3.0 microns in diameter. Numerous en passant swellings were present where the fibers formed a plexus in the area of the IHCs. The MOC fibers followed a similar course in the IGSB and OSL, and within the OSL the fibers had few branches. Within the organ of Corti they traveled apically (up to 70 microns) in the nerve bundles located in the IHC area before they crossed the tunnel of Corti. In the region of the OHCs, 9% of the traceable fibers branched to innervate two to three OHCs while 91% appeared to innervate only one OHC. There was no discernible difference in the distribution of contralateral and ipsilateral MOC projections in terms of cochlear region or outer hair cell rows.

Animals↗
PubMed1991

Extent of salmonellae contamination in breeder hatcheries.

Egg fragments, paper pads from chick boxes, and fluff samples were obtained from six commercial broiler breeder hatcheries and analyzed for the presence and level of salmonellae. Overall, 42 of 380 samples (11.1%) from those hatcheries were contaminated with salmonellae. Salmonellae organisms were detected in 22 of 145 (15.2%), 5 of 100 (4.6%), and 15 of 125 (12%) samples of egg fragments, fluff, and paper pads, respectively. The percentage salmonellae-positive samples from each of the six hatcheries were 1.3, 5.0, 22.5, 11.4, 36.0, and 4.3% respectively. Of the 140 samples randomly selected for enumeration, salmonellae were found in 11 samples. Four of these 11 samples had greater than 10(3) salmonellae per sample, 3 others had greater than 10(2) but less than 10(3), and the remaining 4 had less than 10(2). Salmonella serotypes isolated were S. berta, S. california, S. give, S. hadar, S. mbandaka, S. senftenberg, and S. typhimurium, all of which have previously been isolated from poultry. The incidence and extent of salmonellae-positive samples found in the breeder hatcheries were much less than that previously found in broiler hatcheries. Many factors contribute to the lower incidence and level of salmonellae found in the breeder hatcheries; however both the breeder and broiler hatcheries present critical control points in the prevention of salmonellae contamination during commercial poultry production. The cycle of salmonellae contamination will not likely be broken until contamination at these critical points is eliminated.

Animals↗
PubMed1990

The management of intraocular foreign bodies.

This study of 69 cases of retained intraocular foreign body confirms that eyes with anterior segment foreign bodies fare better than those with posterior segment foreign bodies, that eyes with smaller foreign bodies fare better than those with larger, and that the magnet is the safest method of removal. There is no evidence to support primary vitrectomy in every case of posterior segment foreign body. Computerised tomography is the best method of localisation, but a foreign body can be missed on computerised tomography, and a plain x-ray is still the best method of detection.

Adolescent↗
PubMed1990

Localization and guided removal of soft-tissue foreign bodies with sonography.

Sonography was used to detect, localize, and guide removal of foreign bodies in the soft tissues of the extremities and neck. Twenty localization procedures were performed in 19 patients (12 children and seven adults) with 21 foreign bodies including wood, glass, stone, metal, and lead pencil. Localization was accomplished by using anatomic landmarks, ink marks on the skin, and needle and hemostat markers. Localization was facilitated by the use of small standoff pads that were cut for use on small surfaces. The foreign bodies were visualized as hyperechoic foci with acoustic shadows that were partial or complete depending on the angle of insonation and foreign body composition. Hyperechoic comet-tail artifacts (reverberation artifacts) were seen with six metallic foreign bodies and one glass fragment. Nine foreign bodies were surrounded by hypoechoic halos caused by edema, abscess, or granulation tissue. A slow meticulous scanning technique and high-frequency transducer helped in detection of small foreign bodies. Sonographically guided removal of the foreign body was successful in all four patients in whom it was attempted. Scanning with the ultrasound beam parallel to the long axis of the hemostat and the foreign body was the fastest way to guide the hemostat to the tip of a foreign body. The procedure was accomplished most easily and quickly when one physician performed sonography and hemostat removal of the foreign body simultaneously. Our experience with these cases indicates that sonography is a useful tool in the localization and removal of soft-tissue foreign bodies.

Adolescent↗
PubMed1989

Septal collateralization: demonstration of canine intramyocardial collaterals.

The anatomical distribution of intramyocardial collateral arteries that develop from the septal to the other major coronary arteries was studied in dogs following gradual Ameroid occlusion of the circumflex artery. The septal artery was cannulated and injected with Batson's plastic compound resulting in a cast of the coronary circulation. Collateral vessels radiated from the septal vascular bed to both the circumflex and anterior descending arteries. The collaterals developed from the entire base-to-apex extent of the septal artery and were found on both the right and left sides of the septum. Collateral growth appeared to be more concentrated at the apex of the heart. The anatomical details of septal collateral circulation illustrate the importance of intramyocardial collateralization in the dog, which was thought to exhibit primarily epicardial collaterals.

Animals↗
PubMed1988

Comparative electrocardiographic and myocardial blood flow effects of rapid atrial pacing and dipyridamole infusion in dogs with chronic coronary artery occlusion.

Altering regional myocardial flow by the use of potent vasodilators as well as increasing oxygen demand by exercise have been employed as diagnostic methods for detecting coronary arterial obstruction. We sought to define the relative capabilities of these two methods to produce abnormal ECG patterns and to alter myocardial blood flow after Ameroid constriction of the left circumflex coronary artery. Atrial pacing to rates of 210 beats/min was performed, followed by intravenous administration of 0.25 and 0.50 mg/kg of dipyridamole. ECGs were recorded to construct body surface isopotential distributions. Flow was measured by serial injections of radiolabelled microspheres. In 15 animals studied after 3 to 5 weeks of Ameroid constriction, pacing increased epicardial flow by 19.14 +/- 7.11% but reduced endocardial flow by 35.69 +/- 12.32%, with a significant reduction (to less than 0.67) in the endocardial/epicardial flow ratio in 12 (80%) dogs; both endocardial and epicardial flows were significantly lower in the ischemic than in the nonischemic bed. Ten dogs developed abnormal ST segment responses of subendocardial ischemia. In contrast, dipyridamole produced a dose-dependent rise in both endocardial (25.33 +/- 8.54% and 55.80 +/- 9.22% after 0.25 and 0.5 mg/kg, respectively) and epicardial (29.13 +/- 7.49% and 66.33 +/- 7.64% after 0.25 and 0.5 mg/kg, respectively) flows without a significant fall in the transmural ratio. These increases were, however, significantly less than those observed in nonischemic bed flow, resulting in a transventricular gradient of blood flow. No dog developed abnormal ECG patterns after dipyridamole infusion. Thus pacing produces both a transmural and a transventricular gradient in blood flow, whereas vasodilation causes only a transventricular flow abnormality.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1988

Effects of residual stenosis on infarct size and regional transmural myocardial blood flow after reperfusion.

This study in dogs was designed to determine the effects of residual stenosis on infarct size and on the transmural distribution of coronary flow in the central and peripheral ischemic perfusion bed. A plastic shunt containing a Doppler flow probe was inserted between the left anterior descending coronary artery and the subclavian artery. The dogs were divided into two groups. Group 1 (N = 7) underwent total shunt occlusion for 2 hours followed by reperfusion at 50% of control flow for 2 hours. Group 2 (N = 8) underwent 2 hours of total occlusion followed by 2 hours of total reperfusion. Regional blood flow was measured by radiolabeled microspheres, and infarct areas were quantitated with triphenyl tetrazolium chloride staining. Infarct sizes expressed as a percentage of the left ventricle or as a percentage of perfusion territory were significantly (p less than 0.05) smaller in animals with total reperfusion (group 2) than in dogs with partial reperfusion (group 1). Endocardial flows in the central infarct zone were significantly higher in dogs with total reperfusion than was observed with partial reperfusion; epicardial flows were not significantly different. In the peripheral region both endocardial flows and epicardial flows with total reperfusion were significantly higher than with partial reperfusion. These studies suggest that residual stenosis after thrombolysis may increase infarct size and reduce endocardial flow in the central infarct zone and transmural flow in the peripheral zone.

Animals↗