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Biomedical subjects

J Létourneau

Publications and source records attributed to J Létourneau.

8 recordsLinked to original sources

[The daily public health nursing practice: the Montreal infectious diseases unit as an example].

The health of urban populations depends on multiple factors, among them access to health and social services. The Montreal Public Health Department (PHD) is part of such services, where a number of nurses are working within a multidisciplinary team. It is through the description of the work accomplished by the team for infectious diseases control and prevention, and the vignettes of real life events related to the follow-up of syphilis, tuberculosis and hepatitis A cases, as well as quarantined individual exposed to SARS that the authors want to illustrate the work of the urban public health nurses. The examples are preceded by a description of the socio-demographic characteristics of the city of Montreal, that influence health problems, and therefore nurses' work.

Demography↗

Characterization and expression of the mouse endonuclease G gene.

Endonuclease G (Endo G) is a nuclease of prokaryotic lineage found in the mitochondria of vertebrates that has been suggested to play a role in mitochondrial DNA (mtDNA) replication. We have isolated and sequenced the entire mouse endo G gene, determined the limits of the mRNA, and mapped the promoter region. The coding sequence of the single copy gene is interrupted by two introns and analysis of the transcripts does not support a model by which more than one Endo G isoform could be produced by alternative splicing. We have also characterized a full-length human Endo G cDNA and comparison at the protein level of the human, bovine, and murine nucleases indicates a high degree of conservation except in the respective mitochondrial targeting signals. Endo G is ubiquitously expressed and the steady-state levels of its mRNA vary by a factor greater than seven between different tissues. The relationship between the mtDNA copy number and Endo G mRNA levels is not strictly proportional but tissues richer in mtDNA have higher amounts of the mRNA and vice versa.

Alternative Splicing↗

[Elementary precautions: past the principles].

Universal precautions assure maximum protection to patients, workers, and visitors in a health care institution. While establishing universal precautions is a laudable goal, the application is onerous and difficult if the administration is not convinced of the necessity of such a project. At Sainte Justine Hospital in Montreal, the administration has rallied around this project. A vast program of consultation was initiated in January 1988. Simultaneously, a team of physicians and nurses proceeded to revise standards of sterilization and disinfectation in the hospital. In the fall of 1988, a multidisciplinary team produced a document, Universal Precautions. The term used in French--elementary precautions--was developed to demonstrate the need to have these precautions in place at all times throughout the institution. A pilot project was initiated on the perinatal unit to assess the new standards. Results are very positive. The guidelines worked and people appreciated the diverse measures of protection available to them. A full description of the pilot project is given along with the operating budget.

Cross Infection↗