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J Lajos

Publications and source records attributed to J Lajos.

At least 19 recordsLinked to original sources
PubMed1986

Lymphocyte killer activity in individuals sensitized against antigen-D assessed by direct ADCC lysis of O,Rh-positive erythrocytes.

Lymphocytes from individuals sensitized against the Rh-D antigen were found to lyse O,Rh-positive erythrocytes. The controls consisted of individuals non-sensitized against D antigen. The lytic effect in the absence of additional in vitro added specific antibody was very high in sensitized individuals, but low in the control group. Lytic effect decreased after treating the lymphocytes of sensitized donors with trypsin, but it remained unchanged in the control group. These results suggest that the sensitized donors possessed lymphocytes with specific antibodies in vivo. This phenomenon could prove a valuable diagnostic tool in recognizing responder and non-responder donors in the early phase of immunization.

ABO Blood-Group System↗
PubMed1982

In vitro investigation of Rh antibodies in pregnant women.

Seventeen Rh negative pregnant women who had Rhesus alloantibodies were investigated serologically. The antibody-mediated cellular cytotoxicity (ADCC) against target Rhesus positive human erythrocytes was determined. The investigation was repeated in the first, second and third trimester of pregnancy and after delivery. There was no correlation between ADCC and antibody titre. The antibody titre and the cytotoxic index increased during pregnancy, but the cytotoxic index decreased after delivery. The cytotoxic index showed individual characteristics and was quite independent of the titre of anti-D. There was no correlation between the heavy chain type of anti-D and the cytotoxic index. Our data suggest that the individual variations during pregnancy of the supposed cytotoxic antibody acting together with anti-D play an important role in pregnancy and delivery.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1979

Characteristics of different rhesus alloantibodies in lymphocyte dependent (K cell) cytotoxicity to human erythrocytes.

The property of rhesus alloantibodies to elicit antibody-dependent, cell-mediated cytotoxicity (ADCC) against target erythrocytes carrying various Rh genotypes was studied. The killer activity of normal peripheral lymphocytes on human erythrocyte target cells carrying the appropriate antigens elicited by alloantisera was measured by 51Cr release at 18 h. There was no correlation between ADCC and antibodies directed to the antigens present on the surface of different genotypes of Rh-positive red blood cells. The agglutinin titre of different Rh antibodies showed no correlation with the level of ADCC although the degree of cellular cytotoxicity was different with different anti-D sera. Anti-C + D+ E antibody caused higher ADCC than anti-C + D and the lowest cytotoxicity was observed with anti-D and anti-D+ E. This raised the possibility that ADCC was elicited by antibodies directed to other specificities. K cell lysis of human red cells by human peripheral blood lymphocytes in vitro suggests that a similar mechanism may operate in vitro in the destruction of erythrocytes coated by allo or autoantibodies.

Animals↗
PubMed1975

Investigation of saliva immunoglobulins in monoclonal gammopathies.

Salivary immonoglobulins of patients with multiple myeloma, lymphoid leukaemia, agammaglobulinaemia and of normal healthy adults were examined. Higher IgG level was identified in 4 cases IgG monoclonal gammopathy. In these cases extremely large quantities of IgG monoclonal protein were transmitted from serum into salivary secretions. On the basis of the results it appears that monoclonal IgA is transferred to external secretions in patients with IgA monoclonal gammopathy. High IgM level was found in both serum and saliva of agammaglobulinaemic patients. "IgM secretory component" complex could be identified in saliva. In lymphoid leukaemic patients the salivary immunoglobulins presented normal values except of a patient whose tigA lacked in serum and also in saliva.

Agammaglobulinemia↗
PubMed1975

Determination of the molecular weight of IgM by thin-layer chromatography combined with immunodiffusion in normal and pathological conditions.

An immunodiffusion method combined with thin-layer chromathography was elaborated for the assessment of the molecular weight of IgM. Sera from 100 normal individuals as well as from 162 patients with different types of paraproteinaemia, 100 patients with uveitis and 55 venous umbilical cord sera were analysed. Presence of 75 IgM, besides the usual 19S IgM was detected in 21% of the normal sera, in 27% of the paraproteinaemic sera, in 30% of the uveitic sera and in 5.4% of venous umbilical cord sera. IgM level in the sera was determined by radial immunodiffusion. The authors didn't find correlation between the presence of 7S IgM and different levels of IgM.

Chromatography, Thin Layer↗