Biomedical subjects
Publications and source records attributed to J Lecomte.
The chemical structure of some flavonol derivatives has been correlated with their amino-releasing activity in the rat. Mast-cell amines are liberated when a rhamno-glucose or a glucose molecule is present in--3 position.
Rats pre-treated with the quinazoline-dione derivative Ketanserin (1,25, 2,5 and 5 mg.kg-1, i.p.) (Janssen Pharmaceutica, Beerse, Belgium) present a more prolonged general anaesthesia and a more pronounced incapacity to stand up when injected with pentobarbital (30 to 120 mg.kg-1, i.p.). Cold shivering linked to Ketanserin is also suppressed.
Explore the source record for details and available documents.
A new case of left cervical aorta is described, affecting a 12-year-old girl. Haemodynamic, CT scan and radiological investigations are described. The only associated abnormality in the present case was hypoplasia of the left vertebral artery. There were two spicules on the medial surface of the descending aorta, possibly embryonic rests. A review is made of the literature and there is discussion of the contribution of different diagnostic techniques to determination of the embryological origin or the abnormality and its possible treatment.
A total of 267 passerine birds distributed among 37 species were netted during spring 1980 and summer 1981 in the Laurentian and Montreal areas. All the cloacal swabs collected at that time wer free of influenza viruses. Three and five days after oral administration of avian or human influenza A virus strains, 108 isolates were obtained from 42 of 134 passerine birds. Positive samples were recovered mainly from the respiratory and the digestive tract and also from liver. Spleen and kidneys. Viral replication is cells from trachea, lungs, gizzard and caecum was detected by indirect immunofluorescence using a monoclonal antibody to influenza A virus nucleoprotein. Viral transmission from inoculated to non inoculated birds placed in the same cages was not observed. On the other hand a similar experimental inoculation of young mallard ducks showed that extensive viral transmission occurred from inoculated to non inoculated ducklings and that infection was found exclusively in the digestive tract. Furthermore viruses were detected in samples of drinking water from all cages containing infected ducks. Passerine birds do not represent an important reservoir of influenza viruses but might contribute to the formation and spreading of recombinants potentially pathogenic for man and animals.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Immunoprecipitation of [3H]amino acid labelled virus with monoclonal or human convalescent rubella sera and subsequent analysis by electrophoresis and fluorography, revealed three structural proteins of rubella virus: VP3: 59,000; VP2: 44,800; and VP1: 33,000.
The resolution potential of reverse-phase high-performance liquid chromatography (HPLC) for peptide analysis of hydrophobic viral membranes has been investigated, using as model the membrane (M) protein of influenza virus. Proteolytic digests of 125I-labelled M protein and CNBr fragments, extracted from radioiodinated whole virus, have been separated on a uBondapak C18 column with an isopropanol or acetonitrile solvent system. Peptide mapping of trypsin digests of M protein from A/PR/8/34 (H1N1) and A/chicken/Germany/N/49 (H10N7) viruses was identical, whereas Staphylococcus aureus V8 protease digests showed minor differences in at least two peptides. The results also show that HPLC is a powerful tool for the separation of proteolytic digests of viral proteins, since the peptide maps are highly reproducible and recovery was greater than 85%.
Explore the source record for details and available documents.
In normal pentobarbital-anaesthetized rats (20 mg/kg, i.p.), Ketanserine (2.5 to 10 mg/kg, i.v.) increases the temperature of the paw skin and decreases the central one. A strong thermogenetic shivering appears when central temperature falls below 35 degrees C. This shivering is a specific characteristics of Ketanserine activity, probably related to its fixation on 5-HT2 central receptors.
When gas pockets previously formed in the dorsal subcutaneous tissue of the rat are filled with pure oxygen (F0(2) = 1) no local vascular extravasation is provoked. Blood vessels of the pocket walls do not exhibit the same reactivity as the peritoneal ones to hyperoxic gaseous mixtures.