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Biomedical subjects

J Libcke

Publications and source records attributed to J Libcke.

6 recordsLinked to original sources
PubMed1999

Cyclic guanine monophosphate (cGMP) an intracellular mediator of cavernosal smooth muscle relaxation following penile vascular surgery.

BACKGROUND: We describe a new surgical method for treatment of male impotence. This procedure allows preservation of erectile tissues and avoids the need for implantable devices. It was demonstrated, that after this procedure sustained and reproducible erections were feasible. The role of cyclic guanine monophosphate (cGMP) in generation of erection following this procedure was studied. METHODS: Concentration of cGMP in erectile tissues was studied in the chronic canine experimental model. cGMP measurements were obtained using enzymeimmunoassay method. RESULTS: It was detected that the process of erection was accompanied with increased concentration of cGMP in the involved erectile structures (from 39.59 +/- 8.95 and 39.39 +/- 8.54 pm/g to 78.67 +/- 24.05 and 81.62 +/- 18.80 pm/g respectively with p = 0.0001). Concentration of cGMP did not change in the control (from 41.14 +/- 5.38 to 39.26 +/- 7.59 pm/g with p = 0.4568). CONCLUSION: It can be concluded, that proposed new surgical procedure causes erection not by simple distention of erectile tissue sinuses by arterial blood. Arterial blood inflow is accompanied by activation of intracellular mechanisms responsible for smooth muscle relaxation in erectile tissues. Normal values of cGMP concentration in canine erectile tissues are also reported.

Animals↗
PubMed1997

Arteriovenous fistula combined with basal external penile compression: a new cure for male impotence.

We describe here a new procedure designed to enable reproducible sustained erection in a canine model. In 10 dogs, an end-to-side arteriovenous fistula was created between the inferior epigastric artery and deep dorsal vein. A chronic skin tunnel was created at the penile base. Blood flow, intracavernosal pressure and intraspongiosal pressure and blood gases were measured initially and 3 months later. Cuff application for 30 minutes was used daily. At 3 months, animals were sacrificed and biopsies obtained. Physiologic and histological parameters of erectile structures remained unchanged. Intracavernosal and intraspongiosal pressures increased to 33.7 +/- 6.7 mm Hg and 25.3 +/- 12.2 mm Hg (from 8.0 +/- 2.9 and 6.0 +/- 3.6 mm Hg) after cuff application. We conclude that sustained erections are feasible. This procedure appears safe for human trials.

Anastomosis, Surgical↗
PubMed1994

Hemostasis and healing of superficial splenic injuries using Nd:YAG laser and nonsuture techniques: preliminary report.

This study was designed to compare Nd:YAG laser to fibrin glue, electrocautery, and avitene in the management of superficial splenic injury. Six dogs were submitted to laparotomy. A #11 blade scalpel was used to sharply excise the splenic capsule inflicting four 1" x 1" superficial injuries on each spleen. The lesions were treated. All animals had a second laparotomy ("first relaparotomy"); 2 dogs each were reexplored on postop days 3, 7, and 14. Morphologic and histologic observations were made. A third and final relaparotomy was performed on all dogs at 21 days with repeated morphologic and histologic assessments. Hemostatic times, grades of adhesions, and microscopic changes were not significantly different among the various treatments (P > 0.25). Capsular plaque formations were significantly different at the first relaparotomy (P < 0.01) and at final relaparotomy (P < 0.05). Both adhesions and capsular plaque formation were least at fibrin glue-treated sites, whereas Nd:YAG (1.06 microns) was most effective for average hemostatic time (mean = 109.67 s). Electrocautery produced the greatest necrosis at treatment sites. We conclude that all modalities are effective in controlling hemorrhage.

Animals↗
PubMed1991

Comparison of needle aspiration cytologic diagnosis with excisional biopsy tissue diagnosis of palpable tumors of the breast in a community hospital.

Fine needle aspiration cytologic examination has not extensively been used in our hospital in the work-up evaluation of solid tumors of the breast and its reliability as a basis on which to perform definitive treatment of carcinoma of the breast was in question. One hundred and five aspiration cytologic specimens were obtained from palpable solid tumors of the breast just prior to excisional biopsy. Specimens were numbered and submitted to three different staff pathologists for diagnosis in a single blind manner. Results were then compared with tissue diagnosis of the same tumors. Of 105 specimens taken at biopsy of the breast, 28 malignant lesions were diagnosed on the final tissue report. Of those, ten were diagnosed as malignant (Class V), two were highly suspicious (Class IV) and six were insufficient specimens (Class O). Of those diagnosed as class IV and V by the results of cytologic examination, all were malignant on permanent section for a specificity of 100 per cent and a sensitivity of 36 per cent. The cytologic diagnoses of Classes I, II and III did not correlate with any histologic report, benign or malignant. Malignant growths were misdiagnosed by cytologic examination in 36 per cent and missed because of inadequate specimens in 28 per cent. We believe that a Class V cytologic finding is sufficient basis on which to perform definitive treatment of carcinoma of the breast provided rigid criteria are used. There is a learning curve associated with this diagnostic modality.

Biopsy↗