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Biomedical subjects

J Linz

Publications and source records attributed to J Linz.

7 recordsLinked to original sources
PubMed2000

Novel procedure for identification of compounds inhibitory to transcription of genes involved in mycotoxin biosynthesis.

A novel assay is described for the identification and isolation of compounds that inhibit the transcription of genes involved in mycotoxin biosynthesis. The thin-layer chromatography-based assay was used to screen plant extracts for compounds that would inhibit the expression of the beta-glucuronidase reporter gene under the control of an aflatoxin biosynthesis gene promoter in Aspergillus parasiticus. The assay was used to track purification of an inhibitory compound, cp2, from extracts of black pepper (Piper nigrum). Cp2 did not inhibit mycelial growth or the expression of the beta-tubulin gene but did inhibit aflatoxin biosynthesis at the transcriptional level. Applications of cp2 to the control of mycotoxins are discussed.

Aflatoxins↗
PubMed1996

DNA alterations in sporadic colorectal tumors do not correlate with tumor staging diagnosed by the TNM system.

Blood normal and tumor tissue samples of 23 patients with sporadic colorectal tumors were screened for DNA alterations in the tumor relevant genes APC, K-ras, DCC and p53. Six different microsatellite regions were analyzed for instability by a new developed non-radioactive method. Somatic DNA alterations were found in 17 tumor samples: 13 carried single or multiple changes in single genes; six carried alterations in microsatellites; two tumors showed tumor suppressor gene mutations in addition to microsatellite changes. We found no indications of correlations between current genetic models of colorectal tumor progression and the established TNM system for histopathological tumor classification.

Aged↗
PubMed1985

Analysis of outer membrane proteins which are associated with growth of Bacteroides thetaiotaomicron on chondroitin sulfate.

By analyzing outer membrane proteins of Bacteroides thetaiotaomicron on two-dimensional polyacrylamide gels, we were able to identify 10 protein spots that were associated with growth on chondroitin sulfate but not with growth on glucuronic acid or other monosaccharides. These proteins were distinct from the outer membrane polypeptides that were associated with growth on two other negatively charged polysaccharides, polygalacturonic acid and heparin. Of the 10 protein spots that were associated with growth on chondroitin sulfate, 4 could be detected on immunoblots with antiserum that had been raised against outer membranes from bacteria grown on chondroitin sulfate and then cross-adsorbed with membranes from bacteria grown on glucose. Synthesis of these four proteins appeared to be regulated coordinately with synthesis of the two enzymes that degrade chondroitin sulfate, chondroitin lyase I and II. Although one of the four proteins (Mr 110,000) was similar in molecular weight to the chondroitin lyases, the cross-adsorbed antiserum which detected this outer membrane protein did not cross-react with either of these two enzymes.

Bacterial Outer Membrane Proteins↗
PubMed1985

Home care audits.

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Centers for Medicare and Medicaid Services, U.S.↗
PubMed1979

Investigation of the relationship between adaptation measured binaurally and monaurally.

Recently it has been demonstrated that auditory adaptation can be measured with a monaural technique (Weiler and Friedman, 1973; Weiler and Gross, 1976; Feaster and Weiler, 1975). Because the magnitude of adaptation measured monaurally was nearly identical to that measured binaurally in other experiments, it was assumed that they measured the same phenomenon or phenomena. In the present study the same group of subjects were tested for magnitude of adaptation both monaurally and binaurally. The means were 11.75 dB and 12.17 dB, respectively (N=13). Depsite nearly identical means for the present conditions there was no significant correlation (r=+.27) between the magnitude of adaptation measured under the two conditions. Except for the possibility that individual differences in the ability to do monaural versus binaural tasks may have obscured individual similarities in adaptation, the only other explanation the authors can suggest is that the two techniques measure different aspects of loudness adaptation.

Adult↗