A survey of various media and growth factors used in cell cultivation.
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Biomedical subjects
Publications and source records attributed to J Litwin.
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Human embryonic diploid lung fibroblasts were exposed to various fractions of cigarette smoke condensate over their in vitro life-time. Most fractions were toxic at a concentration of 50 microgram/ml, with the exception of the strong acid and one water soluble basic fraction, which stimulated growth and increased longevity significantly at this concentration. Most fractions produced no effect on cell growth at 10 microgram/ml, with the exception of another basic fraction which inhibited growth at 1 microgram/ml. Nicotine had no apparent effect on growth and longevity at 50 microgram/ml. The neutral fraction containing the polynuclear hydrocarbon carcinogens produced normal growth and longevity at 10 microgram/ml. No cell transformations were observed.
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Using the fluorescence technique the presence of various immunoglobulin classes (IgG, IgA, IgM) was determined on the surface of peripheral blood lymphocytes obtained from healthy subjects (10 cases) and from patients with chronic lymphatic leukaemia (65 cases). In the investigations the effect of treatment and clinical course on the values of these parameters was taken into consideration. Nearly in all cases of this leukaemia the percent of lymphocytes binding immunoglobulins on their surface was raised in comparison with the lymphocytes of healthy subjects. Presence of one or more classes of immunoglobulins was demonstrated on the surface of leukaemic lymphocytes.
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