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Biomedical subjects

J Lizana

Publications and source records attributed to J Lizana.

At least 19 recordsLinked to original sources
PubMed1984

Analysis of prostatic acid phosphatase in urine voided before and after massage of the prostate in infertile men.

Prostatic acid phosphatase (PAP) - a specific marker of prostatic secretion - has been analyzed by radioimmunoassay in samples of urine voided before and after massage of the prostate. In all patients there was an increase in urine PAP concentration after massage irrespective of the occurrence of expressed prostatic secretion. The mean PAP values increased from 4.9 to 45.8 micrograms/l. No significant change in urine creatinine concentration was observed. No lactoferrin was detected in the urine which should indicate that no secretion from the seminal vesicles was expressed into the urethra during massage of the prostate. Analysis of PAP in urine after massage of the prostate could increase the value of a urine sediment and microbial analysis in the investigation of male infertility.

Acid Phosphatase↗
PubMed1984

High resolution isoelectric focusing in a narrow pH interval for the phenotyping of vitamin D-binding protein (Gc-globulin).

A routine method for the analysis of Vitamin D-binding protein (Gc-globulin) phenotypes has been developed. The assay is based on isoelectric focusing in a narrow pH interval (4.5-5.4) using Agarose IEF (1%) or polyacrylamide (T5% C3%) gels, followed by immunofixation. When focused in this pH gradient the anodal and cathodal bands of the phenotype Gc 1-2 are separately by 16-18 mm. The "fast" and "slow" bands of the phenotype Gc 1-1 can also be easily distinguished from each other. The isoelectric focusing technique which has been developed resolves the Vitamin D-binding protein phenotypes in a fast, convenient and reproducible manner. Since agarose gels are so easy to cast and process, Agarose IEF is recommended as the matrix of choice.

Humans↗
PubMed1984

Tissue polypeptide antigen immunoreactivity in human semen.

A substance with TPA immunoreactivity is present in human seminal plasma. This seminal plasma TPA shows immunological similarity with TPA isolated from a pool of carcinoma tumors. TPA is taken up by live spermatozoa and it cannot be easily washed out. Split-ejaculate studies suggest that TPA originates mainly from the prostate. TPA is not correlated with the spermatozoa count, total number of spermatozoa, percentage of normal spermatozoa, or forward motility. The mean seminal plasma concentration of TPA in morphologically normal ejaculates is 14,531 U/liter. No difference was found in the TPA levels between the normal group and various groups of patients under investigation for involuntary infertility.

Adult↗
PubMed1983

Fast chromatofocusing of human serum proteins with special reference to alpha 1-antitrypsin and Gc-globulin.

A new chromatofocusing medium, MonoP, was used for fast (60 min or less) separations of human serum proteins. Separations in the broad pH interval 6.0-3.8 were analysed by fused rocket immunoelectrophoresis to identify a number of proteins, and by gradient gel electrophoresis to determine the molecular weight distribution of the eluted material. To illustrate further the high resolving power of chromatofocusing, narrow pH intervals of about 0.5 pH units were used to study the microheterogeneity of alpha 1-antitrypsin and Gc-globulin. Due to its high resolving power and preparative capacity, chromatofocusing is attractive as the first dimension in two-dimensional techniques for the resolution of complex protein mixtures.

Carrier Proteins↗
PubMed1983

Beta2-microglobulin, carcinoembryonic antigen and prostatic acid phosphatase in split ejaculates and urine voided before and after massage of the prostate.

Analyses of split ejaculates and urine voided before and after prostatic massage have been used to evaluate the origin of beta2-microglobulin (b2m) and carcinoembryonic antigen (CEA) in human semen. CEA and b2m were present in rather high concentrations in all the fractions of the split ejaculates. The urine voided after massage of the prostate also contained increased levels of CEA and beta2-microglobulin. Split ejaculates, selected from the electrophoretic pattern obtained for each fraction, revealed that b2m in seminal plasma originates from the prostate and seminal vesicles in equal amounts. Besides the "free" b2m in seminal plasma, human semen contains a sperm-bound b2m fraction. Carcinoembryonic antigen was found in the highest concentrations in the second fraction of split ejaculates indicating that it originates mainly from the prostate.

Acid Phosphatase↗
PubMed1983

Immunonephelometry of specific proteins in human seminal plasma.

Using immunonephelometry, we measured albumin, transferrin, alpha 1-antitrypsin, orosomucoid, hemopexin, IgG, IgA, and secretory IgA in seminal plasma of men who were attending a fertility clinic. Seminal plasma, obtained by centrifugation of semen, is a turbid liquid producing high blank values that were not diminished by ultracentrifugation, filtration, or treatment with protamine, dextran sulfate, or various lipidophilic gels. Pretreatment of seminal plasma with polyethylene glycol 6000 (final concentration, 40 g/L) considerably decreased the blank values without appreciably changing the relative concentration of the eight proteins studied. Our investigations illustrate the feasibility of this application of immunonephelometry. This approach can be a valuable diagnostic tool in the laboratory evaluation of the male partner in infertile unions.

Adult↗
PubMed1982

Proteins of human amniotic fluid. II. Mapping by two-dimensional electrophoresis.

In an earlier study we used various electrophoretic techniques to investigate the proteins in amniotic fluid, including two-dimensional electrophoresis. However, the high proportion of albumin dominated the pattern and tended to distort the pH gradient. Improved methodology, based upon the removal of albumin with Blue Sepharose CL6B and the silver-staining technique, has been used in the present work. These modifications have minimized problems of distortion. We have extended our work in a attempt to complete the two-dimensional map of the proteins in amniotic fluid. Over 200 proteins were seen, ranging in molecular mass from 10 000 to 100 000, including a relatively high proportion of polypeptides of low-molecular mass. The spots seen in the map are discussed in relation to some of the proteins, peptides, and hormones that have been reported in amniotic fluid. Eventually, it is hoped that polypeptides will be found that will provide better indicators of the condition of the fetus.

Albumins↗
PubMed1981

The occurrence of immunoglobulin E in Human seminal plasma.

The presence of IgE in human seminal plasma has been explored using two different radioimmunoassay methods. Samples were obtained from 84 patients under investigation for involuntary infertility. Low levels were recorded in seminal plasma (less than 0.05-805 kIU/1) together with a wide scatter of serum values (less than 0.5-956 kIU/1). The incidence of detectable seminal plasma IgE was related to high serum IgE values (P less than 0.001; chi 2-test). Among men reporting allergy problems and who has serum IgE levels above 50 kIU/1 detectable semen IgE was seen in 15 out of 18 patients. Sperm agglutination was observed in 18 men of whom 11 had detectable seminal plasma IgE. The question of whether or not seminal plasma IgE is a plasma transudate or is locally produced as well as its possible function in seminal plasma is discussed.

Female↗
PubMed1977

Assessment of five quantitative methods for determination of total proteins in urine.

1. We assessed five quantitative methods for the determination of urinary total proteins, three of them with precipitation followed by spectrophotomollowed by spectrophotometry (Tsuchyga/Benedict, Tsuchyga/Biuret and TCA/Biuret); one with gel-filtration (Sephadex G50/Biuret) and a Turbidimetric procedure (SSA). 2. Day-to-day precision was between 3.58 %CV (Tsuchyga/Benedict) and 11.46 %CV (Sephadex/Biuret). The Tsuchyga/Benedict method showed the closest values to controls quantitated by the Kjeldahl method and the lowest detection limit )17.3 mg of total proteins per liter). Recovery studies showed that a level of 100-200 mg/l the Tsuchyga/Benedict recovered over the 96% of the protein added. 3. Comparison studies of 66 24-hour urine samples showed that the best correlation was obtained between the Tsuchyga/Benedict and the Tsuchyga/Biuret methods (r = 0.996). 4. We conclude that the Tsuchyga/Benedict is a sensitive, precise and accurate procedure for the routine quantitation of urinary total proteins.

Biuret Reaction↗
PubMed1975

Electronic timing of a high-speed immunonephelometric continuous-flow system.

We describe a high-speed immunoephelometric continuous-flow system. The mechanical timing asembly and cams of the sampler were replaced by an external electronic control unit. Improved sampling precision, speeds of 2 to 900 samples per hour, and a wide variety of sample-to-wash ratios are the most important advantages of the unit. We analyzed albumin and fibrinogen at speeds of 150, 180, and 210 samples per hour. Carry-over studies showed that, in clinical practice, it should be possible to run the system at 150 samples per hour with an interaction of less than 5% and a precision (CV) of around 4%. Comparative studies with a previously described method, run at 60 samples per hour, showed correlation coefficients of 0.968 and 0.935 for albumin and fibrinogen, respectively. The electronic control unit can be used to increase speed and improve sampling precision of any continuous-flow system. An immunonephelometric system equipped with this device provided fast and reliable results, and required less antiserum than other systems described.

Animals↗